Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts
Skeletal muscle is an essential tissue in meat-producing animals, and meat-producing traits have been a hot topic in chicken genetic breeding research. Current research shows that <i>creatine kinase M-type-like</i> (<i>CKM</i>) is one of the most abundant proteins in skeletal...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/14/2316 |
| _version_ | 1827734228933017600 |
|---|---|
| author | Bingjie Chen Yanxing Wang Dan Hou Yushi Zhang Bochun Zhang Yufang Niu Haigang Ji Yadong Tian Xiaojun Liu Xiangtao Kang Hanfang Cai Zhuanjian Li |
| author_facet | Bingjie Chen Yanxing Wang Dan Hou Yushi Zhang Bochun Zhang Yufang Niu Haigang Ji Yadong Tian Xiaojun Liu Xiangtao Kang Hanfang Cai Zhuanjian Li |
| author_sort | Bingjie Chen |
| collection | DOAJ |
| description | Skeletal muscle is an essential tissue in meat-producing animals, and meat-producing traits have been a hot topic in chicken genetic breeding research. Current research shows that <i>creatine kinase M-type-like</i> (<i>CKM</i>) is one of the most abundant proteins in skeletal muscle and plays an important role in the growth and development of skeletal muscle, but its role in the development of chicken skeletal muscle is still unclear. Via RNA sequencing (RNA-seq), we found that <i>CKM</i> was highly expressed in chicken breast muscle tissue. In this study, the expression profile of <i>CKM</i> was examined by quantitative real-time PCR (qPCR), and overexpression and RNA interference techniques were used to explore the functions of <i>CKM</i> in the proliferation, apoptosis and differentiation of chicken primary myoblasts (CPMs). It was shown that <i>CKM</i> was specifically highly expressed in breast muscle and leg muscle and was highly expressed in stage 16 embryonic muscle, while <i>CKM</i> inhibited proliferation, promoted the apoptosis and differentiation of CPMs and was involved in regulating chicken myogenesis. Transcriptome sequencing was used to identify genes that were differentially expressed in CPMs after <i>CKM</i> disruption, and bioinformatics analysis showed that <i>CKM</i> was involved in regulating chicken myogenesis. In summary, <i>CKM</i> plays an important role in skeletal muscle development during chicken growth and development. |
| first_indexed | 2024-03-11T01:22:24Z |
| format | Article |
| id | doaj.art-4dc415edd4f342ab946a5640275393b8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:22:24Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4dc415edd4f342ab946a5640275393b82023-11-18T18:00:35ZengMDPI AGAnimals2076-26152023-07-011314231610.3390/ani13142316Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary MyoblastsBingjie Chen0Yanxing Wang1Dan Hou2Yushi Zhang3Bochun Zhang4Yufang Niu5Haigang Ji6Yadong Tian7Xiaojun Liu8Xiangtao Kang9Hanfang Cai10Zhuanjian Li11College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, ChinaSkeletal muscle is an essential tissue in meat-producing animals, and meat-producing traits have been a hot topic in chicken genetic breeding research. Current research shows that <i>creatine kinase M-type-like</i> (<i>CKM</i>) is one of the most abundant proteins in skeletal muscle and plays an important role in the growth and development of skeletal muscle, but its role in the development of chicken skeletal muscle is still unclear. Via RNA sequencing (RNA-seq), we found that <i>CKM</i> was highly expressed in chicken breast muscle tissue. In this study, the expression profile of <i>CKM</i> was examined by quantitative real-time PCR (qPCR), and overexpression and RNA interference techniques were used to explore the functions of <i>CKM</i> in the proliferation, apoptosis and differentiation of chicken primary myoblasts (CPMs). It was shown that <i>CKM</i> was specifically highly expressed in breast muscle and leg muscle and was highly expressed in stage 16 embryonic muscle, while <i>CKM</i> inhibited proliferation, promoted the apoptosis and differentiation of CPMs and was involved in regulating chicken myogenesis. Transcriptome sequencing was used to identify genes that were differentially expressed in CPMs after <i>CKM</i> disruption, and bioinformatics analysis showed that <i>CKM</i> was involved in regulating chicken myogenesis. In summary, <i>CKM</i> plays an important role in skeletal muscle development during chicken growth and development.https://www.mdpi.com/2076-2615/13/14/2316<i>CKM</i>chicken primary myoblastsproliferationapoptosisdifferentiationRNA sequencing |
| spellingShingle | Bingjie Chen Yanxing Wang Dan Hou Yushi Zhang Bochun Zhang Yufang Niu Haigang Ji Yadong Tian Xiaojun Liu Xiangtao Kang Hanfang Cai Zhuanjian Li Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts Animals <i>CKM</i> chicken primary myoblasts proliferation apoptosis differentiation RNA sequencing |
| title | Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts |
| title_full | Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts |
| title_fullStr | Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts |
| title_full_unstemmed | Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts |
| title_short | Transcriptome-Based Identification of the Muscle Tissue-Specific Expression Gene <i>CKM</i> and Its Regulation of Proliferation, Apoptosis and Differentiation in Chicken Primary Myoblasts |
| title_sort | transcriptome based identification of the muscle tissue specific expression gene i ckm i and its regulation of proliferation apoptosis and differentiation in chicken primary myoblasts |
| topic | <i>CKM</i> chicken primary myoblasts proliferation apoptosis differentiation RNA sequencing |
| url | https://www.mdpi.com/2076-2615/13/14/2316 |
| work_keys_str_mv | AT bingjiechen transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT yanxingwang transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT danhou transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT yushizhang transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT bochunzhang transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT yufangniu transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT haigangji transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT yadongtian transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT xiaojunliu transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT xiangtaokang transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT hanfangcai transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts AT zhuanjianli transcriptomebasedidentificationofthemuscletissuespecificexpressiongeneickmianditsregulationofproliferationapoptosisanddifferentiationinchickenprimarymyoblasts |