Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia
Background: <i>FLT1</i> is one of the significantly overexpressed genes found in a pre-eclamptic placenta and is involved with the etiology of this disease. Methods: We conducted genome-wide expression profiling by RNA-seq of placentas from women with pre-eclampsia and those with normote...
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MDPI AG
2022-08-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/15/4603 |
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| author | Hikari Yoshizawa Haruki Nishizawa Hidehito Inagaki Keisuke Hitachi Akiko Ohwaki Yoshiko Sakabe Mayuko Ito Kunihiro Tsuchida Takao Sekiya Takuma Fujii Hiroki Kurahashi |
| author_facet | Hikari Yoshizawa Haruki Nishizawa Hidehito Inagaki Keisuke Hitachi Akiko Ohwaki Yoshiko Sakabe Mayuko Ito Kunihiro Tsuchida Takao Sekiya Takuma Fujii Hiroki Kurahashi |
| author_sort | Hikari Yoshizawa |
| collection | DOAJ |
| description | Background: <i>FLT1</i> is one of the significantly overexpressed genes found in a pre-eclamptic placenta and is involved with the etiology of this disease. Methods: We conducted genome-wide expression profiling by RNA-seq of placentas from women with pre-eclampsia and those with normotensive pregnancy. Results: We identified a lncRNA gene, <i>MG828507</i>, located ~80 kb upstream of the <i>FLT1</i> gene in a head-to-head orientation, which was overexpressed in the pre-eclamptic placenta. <i>MG828507</i> and <i>FLT1</i> are located within the same topologically associated domain in the genome. The <i>MG828507</i> mRNA level correlated with that of the <i>FLT1</i> in placentas from pre-eclamptic women as well as in samples from uncomplicated pregnancies. However, neither the overexpression nor knockdown of <i>MG828507</i> affected the expression of <i>FLT1</i>. Analysis of pre-eclampsia-linking genetic variants at this locus suggested that the placental genotype of one variant was associated with the expression of <i>MG828507</i>. The <i>MG828507</i> transcript level was not found to be associated with maternal blood pressure, but showed a relationship with birth and placental weights, suggesting that this lncRNA might be one of the pivotal placental factors in pre-eclampsia. Conclusion: Further characterization of the <i>MG828507</i> gene may elucidate the etiological roles of the <i>MG828507</i> and <i>FLT1</i> genes in pre-eclampsia in a genomic context. |
| first_indexed | 2024-03-09T10:07:19Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T10:07:19Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-4dc5129c9295438eb9b80c2330cb4bb72023-12-01T23:00:19ZengMDPI AGJournal of Clinical Medicine2077-03832022-08-011115460310.3390/jcm11154603Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-EclampsiaHikari Yoshizawa0Haruki Nishizawa1Hidehito Inagaki2Keisuke Hitachi3Akiko Ohwaki4Yoshiko Sakabe5Mayuko Ito6Kunihiro Tsuchida7Takao Sekiya8Takuma Fujii9Hiroki Kurahashi10Department of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDivision for Therapies against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Fujita Health University, Toyoake 470-1192, JapanDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanBackground: <i>FLT1</i> is one of the significantly overexpressed genes found in a pre-eclamptic placenta and is involved with the etiology of this disease. Methods: We conducted genome-wide expression profiling by RNA-seq of placentas from women with pre-eclampsia and those with normotensive pregnancy. Results: We identified a lncRNA gene, <i>MG828507</i>, located ~80 kb upstream of the <i>FLT1</i> gene in a head-to-head orientation, which was overexpressed in the pre-eclamptic placenta. <i>MG828507</i> and <i>FLT1</i> are located within the same topologically associated domain in the genome. The <i>MG828507</i> mRNA level correlated with that of the <i>FLT1</i> in placentas from pre-eclamptic women as well as in samples from uncomplicated pregnancies. However, neither the overexpression nor knockdown of <i>MG828507</i> affected the expression of <i>FLT1</i>. Analysis of pre-eclampsia-linking genetic variants at this locus suggested that the placental genotype of one variant was associated with the expression of <i>MG828507</i>. The <i>MG828507</i> transcript level was not found to be associated with maternal blood pressure, but showed a relationship with birth and placental weights, suggesting that this lncRNA might be one of the pivotal placental factors in pre-eclampsia. Conclusion: Further characterization of the <i>MG828507</i> gene may elucidate the etiological roles of the <i>MG828507</i> and <i>FLT1</i> genes in pre-eclampsia in a genomic context.https://www.mdpi.com/2077-0383/11/15/4603lncRNAFLT1placentapre-eclampsia |
| spellingShingle | Hikari Yoshizawa Haruki Nishizawa Hidehito Inagaki Keisuke Hitachi Akiko Ohwaki Yoshiko Sakabe Mayuko Ito Kunihiro Tsuchida Takao Sekiya Takuma Fujii Hiroki Kurahashi Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia Journal of Clinical Medicine lncRNA FLT1 placenta pre-eclampsia |
| title | Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia |
| title_full | Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia |
| title_fullStr | Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia |
| title_full_unstemmed | Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia |
| title_short | Characterization of the <i>MG828507</i> lncRNA Located Upstream of the <i>FLT1</i> Gene as an Etiology for Pre-Eclampsia |
| title_sort | characterization of the i mg828507 i lncrna located upstream of the i flt1 i gene as an etiology for pre eclampsia |
| topic | lncRNA FLT1 placenta pre-eclampsia |
| url | https://www.mdpi.com/2077-0383/11/15/4603 |
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