Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity
The Pacific white shrimp (Litopenaeus vannamei), a euryhaline penaeid species, can tolerate a wide range of salinities, but little is known on its strategies to cope with low salinity fluctuations from the aspect of lipidomics. Thus, in this study, L. vannamei were grown in two different salinities...
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| Format: | Article |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.01087/full |
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| author | Maoxian Huang Maoxian Huang Yangfan Dong Yan Zhang Qinsheng Chen Jia Xie Chang Xu Qun Zhao Erchao Li Erchao Li |
| author_facet | Maoxian Huang Maoxian Huang Yangfan Dong Yan Zhang Qinsheng Chen Jia Xie Chang Xu Qun Zhao Erchao Li Erchao Li |
| author_sort | Maoxian Huang |
| collection | DOAJ |
| description | The Pacific white shrimp (Litopenaeus vannamei), a euryhaline penaeid species, can tolerate a wide range of salinities, but little is known on its strategies to cope with low salinity fluctuations from the aspect of lipidomics. Thus, in this study, L. vannamei were grown in two different salinities [3 and 30‰ (control)] for 8 weeks, and then an liquid chromatography (LC)–mass spectrometry (MS)-based lipidomics analysis was performed to reveal the lipid profile differences in gill and muscle. L. vannamei under low salinity had lower weight gain and condition factor than the control shrimp at 30‰, but no differences were found in survival and hepatopancreas index. A higher number of differential lipid metabolites were identified in gill than in muscle in L. vannamei at salinity 3‰ relative to the control shrimp at salinity of 30‰ (159 versus 37), which belonged to 11 and 6 lipids classes, respectively. Of these lipids, phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylethanolamine (PE), and triglyceride (TG) were the main lipids in both shrimp gill and muscle, regardless of salinities. Compared with the control shrimp at salinity 30‰, the percentage of PC significantly reduced, but TG and PA significantly increased in gill of shrimp at salinity 3‰. Moreover, the relative fatty acid abundances showed significant changes in L. vannamei between the two salinity groups, but the patterns of the changes were complex and were fatty acid dependent. Neither lipid nor fatty acid composition in muscle was affected by salinity. Further pathway analysis showed that these metabolites were closely related to lipid and fatty acid metabolic pathways. All the findings in this study reveal that the lipid variations are closely related to bio-membrane structure, mitochondrial function, energy supply, or organic osmolyte contents in hemolymph for improving osmoregulatory capacity of L. vannamei under low salinity. |
| first_indexed | 2024-12-10T13:26:41Z |
| format | Article |
| id | doaj.art-4dc5148153204d4baa900128258c5847 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T13:26:41Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-4dc5148153204d4baa900128258c58472022-12-22T01:47:07ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-08-011010.3389/fphys.2019.01087468362Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low SalinityMaoxian Huang0Maoxian Huang1Yangfan Dong2Yan Zhang3Qinsheng Chen4Jia Xie5Chang Xu6Qun Zhao7Erchao Li8Erchao Li9Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, ChinaDepartment of Aquaculture, College of Marine Sciences, Hainan University, Haikou, ChinaThe Pacific white shrimp (Litopenaeus vannamei), a euryhaline penaeid species, can tolerate a wide range of salinities, but little is known on its strategies to cope with low salinity fluctuations from the aspect of lipidomics. Thus, in this study, L. vannamei were grown in two different salinities [3 and 30‰ (control)] for 8 weeks, and then an liquid chromatography (LC)–mass spectrometry (MS)-based lipidomics analysis was performed to reveal the lipid profile differences in gill and muscle. L. vannamei under low salinity had lower weight gain and condition factor than the control shrimp at 30‰, but no differences were found in survival and hepatopancreas index. A higher number of differential lipid metabolites were identified in gill than in muscle in L. vannamei at salinity 3‰ relative to the control shrimp at salinity of 30‰ (159 versus 37), which belonged to 11 and 6 lipids classes, respectively. Of these lipids, phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylethanolamine (PE), and triglyceride (TG) were the main lipids in both shrimp gill and muscle, regardless of salinities. Compared with the control shrimp at salinity 30‰, the percentage of PC significantly reduced, but TG and PA significantly increased in gill of shrimp at salinity 3‰. Moreover, the relative fatty acid abundances showed significant changes in L. vannamei between the two salinity groups, but the patterns of the changes were complex and were fatty acid dependent. Neither lipid nor fatty acid composition in muscle was affected by salinity. Further pathway analysis showed that these metabolites were closely related to lipid and fatty acid metabolic pathways. All the findings in this study reveal that the lipid variations are closely related to bio-membrane structure, mitochondrial function, energy supply, or organic osmolyte contents in hemolymph for improving osmoregulatory capacity of L. vannamei under low salinity.https://www.frontiersin.org/article/10.3389/fphys.2019.01087/fullsalinityLitopenaeus vannameilipidomicsgrowthgillmuscle |
| spellingShingle | Maoxian Huang Maoxian Huang Yangfan Dong Yan Zhang Qinsheng Chen Jia Xie Chang Xu Qun Zhao Erchao Li Erchao Li Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity Frontiers in Physiology salinity Litopenaeus vannamei lipidomics growth gill muscle |
| title | Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity |
| title_full | Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity |
| title_fullStr | Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity |
| title_full_unstemmed | Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity |
| title_short | Growth and Lipidomic Responses of Juvenile Pacific White Shrimp Litopenaeus vannamei to Low Salinity |
| title_sort | growth and lipidomic responses of juvenile pacific white shrimp litopenaeus vannamei to low salinity |
| topic | salinity Litopenaeus vannamei lipidomics growth gill muscle |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.01087/full |
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