Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq
It has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastro...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2022-06-01
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| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | https://archrazi.areeo.ac.ir/article_126762_2be1c27fc48e35f686e5a48b52148847.pdf |
| _version_ | 1811318912357236736 |
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| author | F Najd Obaid K. A Al-Dabbagh Y Salam Karim O Abdallah Mahdi A Mahdi Rheima O. K. A. Alkadir D. A Hamad E Mahmood Shihab N Hussin Alwan E. R Alwaily |
| author_facet | F Najd Obaid K. A Al-Dabbagh Y Salam Karim O Abdallah Mahdi A Mahdi Rheima O. K. A. Alkadir D. A Hamad E Mahmood Shihab N Hussin Alwan E. R Alwaily |
| author_sort | F Najd Obaid |
| collection | DOAJ |
| description | It has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of E. coli O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC). The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were E. coli, while the recorded data obtained from m-PCR assay revealed that none of the isolated E. coli was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: O157, H7, stx1, stx2 genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of E. coli O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with E. coli O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region. |
| first_indexed | 2024-04-13T12:33:29Z |
| format | Article |
| id | doaj.art-4dc62c2c3f684a37a711d43a3ee7e187 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T12:33:29Z |
| publishDate | 2022-06-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-4dc62c2c3f684a37a711d43a3ee7e1872022-12-22T02:46:43ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722022-06-017731261126510.22092/ari.2022.358832.2317126762Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, IraqF Najd Obaid0K. A Al-Dabbagh1Y Salam Karim2O Abdallah Mahdi3A Mahdi Rheima4O. K. A. Alkadir5D. A Hamad6E Mahmood Shihab7N Hussin Alwan8E. R Alwaily9Altoosi University College, Najaf, IraqDepartment of Dentistry, Al-Hadba University College, Mosul, IraqDepartment of Pharmacy, Al-Manara College For Medical Sciences, Maysan, IraqAnesthesia Techniques Department, Al-Mustaqbal University College, Babylon, IraqCollege of Technical Engineering, The Islamic University, Najaf, IraqAl-Nisour University College, Baghdad, IraqNursing Department, Hilla University College, Babylon, IraqAl-Esraa University College, Baghdad, IraqDepartment of Nursing, Al-Zahrawi University College, Karbala, IraqMicrobiology Research Group , College of Pharmacy, Al-Ayen University, Thi-Qar, IraqIt has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of E. coli O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC). The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were E. coli, while the recorded data obtained from m-PCR assay revealed that none of the isolated E. coli was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: O157, H7, stx1, stx2 genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of E. coli O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with E. coli O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region.https://archrazi.areeo.ac.ir/article_126762_2be1c27fc48e35f686e5a48b52148847.pdfmilkescherichia coli o157:h7polymerase chain reaction |
| spellingShingle | F Najd Obaid K. A Al-Dabbagh Y Salam Karim O Abdallah Mahdi A Mahdi Rheima O. K. A. Alkadir D. A Hamad E Mahmood Shihab N Hussin Alwan E. R Alwaily Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq Archives of Razi Institute milk escherichia coli o157:h7 polymerase chain reaction |
| title | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
| title_full | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
| title_fullStr | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
| title_full_unstemmed | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
| title_short | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
| title_sort | presence of escherichia coli o157 h7 in dairy farms located in najaf baghdad kirkuk and erbil iraq |
| topic | milk escherichia coli o157:h7 polymerase chain reaction |
| url | https://archrazi.areeo.ac.ir/article_126762_2be1c27fc48e35f686e5a48b52148847.pdf |
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