Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol
The chiral separation of enantiomers is crucial for pharmacovigilance within drug discovery. Although a large number of prescribed medications are marketed as pure enantiomers, this is not always the case and many are in fact racemic mixtures. Drug scandals, such as that of Thalidomide in 1961, prov...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2019-06-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2019-0049 |
| _version_ | 1819125511399407616 |
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| author | Guerrero M.M. López Díaz A. Navas Sánchez F. García Corrall H. |
| author_facet | Guerrero M.M. López Díaz A. Navas Sánchez F. García Corrall H. |
| author_sort | Guerrero M.M. López |
| collection | DOAJ |
| description | The chiral separation of enantiomers is crucial for pharmacovigilance within drug discovery. Although a large number of prescribed medications are marketed as pure enantiomers, this is not always the case and many are in fact racemic mixtures. Drug scandals, such as that of Thalidomide in 1961, provide a clear example of the social and economic repercussions that can be caused by negligence of these chiral compounds. Two high performance liquid chromatography (HPLC) methods are presented to determine, separate and quantitate a commonly prescribed chiral beta blocker, (-)-Alprenolol. The first method utilises a chiral column to physically separate the two enantiomers of Alprenolol in 25 minutes, before quantitating with two detectors. Fluorimetry gave the better limit of detection of 0.16-0.41ng and a correlation coefficient of 0.999. The second method used an achiral column coupled with polarimetry to quantitate (-)-Alprenolol without the need for physical separation in 10 minutes. The limit of detection achieved was 27-37μg and demonstrated a correlation coefficient of -0.999. |
| first_indexed | 2024-12-22T07:41:18Z |
| format | Article |
| id | doaj.art-4dc7ad13b3c84290beafacacc5cdc102 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-22T07:41:18Z |
| publishDate | 2019-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-4dc7ad13b3c84290beafacacc5cdc1022022-12-21T18:33:44ZengDe GruyterOpen Chemistry2391-54202019-06-0117142943710.1515/chem-2019-0049chem-2019-0049Chiral and Achiral Enantiomeric Separation of (±)-AlprenololGuerrero M.M. López0Díaz A. Navas1Sánchez F. García2Corrall H.3Faculty of Sciences, University of Málaga, Av. Cervantes, 2, 29071Málaga, SpainFaculty of Sciences, University of Málaga, Av. Cervantes, 2, 29071Málaga, SpainFaculty of Sciences, University of Málaga, Av. Cervantes, 2, 29071Málaga, SpainFaculty of Sciences, University of Málaga, Av. Cervantes, 2, 29071Málaga, SpainThe chiral separation of enantiomers is crucial for pharmacovigilance within drug discovery. Although a large number of prescribed medications are marketed as pure enantiomers, this is not always the case and many are in fact racemic mixtures. Drug scandals, such as that of Thalidomide in 1961, provide a clear example of the social and economic repercussions that can be caused by negligence of these chiral compounds. Two high performance liquid chromatography (HPLC) methods are presented to determine, separate and quantitate a commonly prescribed chiral beta blocker, (-)-Alprenolol. The first method utilises a chiral column to physically separate the two enantiomers of Alprenolol in 25 minutes, before quantitating with two detectors. Fluorimetry gave the better limit of detection of 0.16-0.41ng and a correlation coefficient of 0.999. The second method used an achiral column coupled with polarimetry to quantitate (-)-Alprenolol without the need for physical separation in 10 minutes. The limit of detection achieved was 27-37μg and demonstrated a correlation coefficient of -0.999.https://doi.org/10.1515/chem-2019-0049separationenantiomerchiralachiralhplc |
| spellingShingle | Guerrero M.M. López Díaz A. Navas Sánchez F. García Corrall H. Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol Open Chemistry separation enantiomer chiral achiral hplc |
| title | Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol |
| title_full | Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol |
| title_fullStr | Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol |
| title_full_unstemmed | Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol |
| title_short | Chiral and Achiral Enantiomeric Separation of (±)-Alprenolol |
| title_sort | chiral and achiral enantiomeric separation of alprenolol |
| topic | separation enantiomer chiral achiral hplc |
| url | https://doi.org/10.1515/chem-2019-0049 |
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