Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice
Abstract Peri-implantitis may result in the loss of dental implants. Cold atmospheric pressure plasma (CAP) was suggested to promote re-osseointegration, decrease antimicrobial burden, and support wound healing. However, the long-term risk assessment of CAP treatment in the oral cavity has not been...
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| Format: | Article |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99924-3 |
| _version_ | 1818838008451825664 |
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| author | K. Evert T. Kocher A. Schindler M. Müller K. Müller C. Pink B. Holtfreter A. Schmidt F. Dombrowski A. Schubert T. von Woedtke S. Rupf D. F. Calvisi S. Bekeschus L. Jablonowski |
| author_facet | K. Evert T. Kocher A. Schindler M. Müller K. Müller C. Pink B. Holtfreter A. Schmidt F. Dombrowski A. Schubert T. von Woedtke S. Rupf D. F. Calvisi S. Bekeschus L. Jablonowski |
| author_sort | K. Evert |
| collection | DOAJ |
| description | Abstract Peri-implantitis may result in the loss of dental implants. Cold atmospheric pressure plasma (CAP) was suggested to promote re-osseointegration, decrease antimicrobial burden, and support wound healing. However, the long-term risk assessment of CAP treatment in the oral cavity has not been addressed. Treatment with two different CAP devices was compared against UV radiation, carcinogen administration, and untreated conditions over 12 months. Histological analysis of 406 animals revealed that repeated CAP exposure did not foster non-invasive lesions or squamous cell carcinoma (SCCs). Carcinogen administration promoted non-invasive lesions and SCCs. Molecular analysis by a qPCR screening of 144 transcripts revealed distinct inflammatory profiles associated with each treatment regimen. Interestingly, CAP treatment of carcinogen-challenged mucosa did not promote but instead left unchanged or reduced the proportion of non-invasive lesions and SCC formation. In conclusion, repeated CAP exposure of murine oral mucosa was well tolerated, and carcinogenic effects did not occur, motivating CAP applications in patients for dental and implant treatments in the future. |
| first_indexed | 2024-12-19T03:31:34Z |
| format | Article |
| id | doaj.art-4dc9c4c21f244e01a7d84ba812f70ff9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T03:31:34Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4dc9c4c21f244e01a7d84ba812f70ff92022-12-21T20:37:29ZengNature PortfolioScientific Reports2045-23222021-10-0111111310.1038/s41598-021-99924-3Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in miceK. Evert0T. Kocher1A. Schindler2M. Müller3K. Müller4C. Pink5B. Holtfreter6A. Schmidt7F. Dombrowski8A. Schubert9T. von Woedtke10S. Rupf11D. F. Calvisi12S. Bekeschus13L. Jablonowski14Institute of Pathology, University of RegensburgDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldLeibniz Institute of Surface Modification (IOM Leipzig)Institute of Pathology, University of RegensburgCenter for Clinical Studies, University Hospital RegensburgDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP)Institute of Pathology, University Medicine GreifswaldDepartment of Immunology, Fraunhofer Institute for Cell Therapy and Immunology (IZI)ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP)Clinic of Operative Dentistry, Saarland UniversityInstitute of Pathology, University of RegensburgZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP)Department of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldAbstract Peri-implantitis may result in the loss of dental implants. Cold atmospheric pressure plasma (CAP) was suggested to promote re-osseointegration, decrease antimicrobial burden, and support wound healing. However, the long-term risk assessment of CAP treatment in the oral cavity has not been addressed. Treatment with two different CAP devices was compared against UV radiation, carcinogen administration, and untreated conditions over 12 months. Histological analysis of 406 animals revealed that repeated CAP exposure did not foster non-invasive lesions or squamous cell carcinoma (SCCs). Carcinogen administration promoted non-invasive lesions and SCCs. Molecular analysis by a qPCR screening of 144 transcripts revealed distinct inflammatory profiles associated with each treatment regimen. Interestingly, CAP treatment of carcinogen-challenged mucosa did not promote but instead left unchanged or reduced the proportion of non-invasive lesions and SCC formation. In conclusion, repeated CAP exposure of murine oral mucosa was well tolerated, and carcinogenic effects did not occur, motivating CAP applications in patients for dental and implant treatments in the future.https://doi.org/10.1038/s41598-021-99924-3 |
| spellingShingle | K. Evert T. Kocher A. Schindler M. Müller K. Müller C. Pink B. Holtfreter A. Schmidt F. Dombrowski A. Schubert T. von Woedtke S. Rupf D. F. Calvisi S. Bekeschus L. Jablonowski Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice Scientific Reports |
| title | Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| title_full | Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| title_fullStr | Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| title_full_unstemmed | Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| title_short | Repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| title_sort | repeated exposure of the oral mucosa over 12 months with cold plasma is not carcinogenic in mice |
| url | https://doi.org/10.1038/s41598-021-99924-3 |
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