Plasma as alternatively sample to quantify tetanus antitoxin
Tetanus antitoxin is quantified in Cuba at blood banks, from the serum of immunized donors, to produce aspecific human gamma globulin. A heterogeneous indirect immunoenzymatic assay is used, using the serum as analytical sample. The possible use of plasma obtained from plasmapheresis as alternative...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Finlay Ediciones
2015-08-01
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| Series: | VacciMonitor |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100007&lng=es&nrm=iso |
| _version_ | 1818527119240593408 |
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| author | Ariel Menéndez-Barrios Pedro Sánchez-Frenes María de Jesús Sánchez-Bouza Andrés Alemañi-Co Sandra Gómez-Fonseca Rodney Kidman Nieves-Armas |
| author_facet | Ariel Menéndez-Barrios Pedro Sánchez-Frenes María de Jesús Sánchez-Bouza Andrés Alemañi-Co Sandra Gómez-Fonseca Rodney Kidman Nieves-Armas |
| author_sort | Ariel Menéndez-Barrios |
| collection | DOAJ |
| description | Tetanus antitoxin is quantified in Cuba at blood banks, from the serum of immunized donors, to produce aspecific human gamma globulin. A heterogeneous indirect immunoenzymatic assay is used, using the serum as analytical sample. The possible use of plasma obtained from plasmapheresis as alternative sample was evaluated in this research, to minimize the volume of total blood extracted to the donors. One hundred plasma donors who came to donate between October and November 2013 were selected by simple random sampling.
Serum sample was obtained for extraction of 5 mL of blood, deposited in dry glass tube. While the other sample took 1.5 mL of plasma in a plastic tube with cover, at the end of the donation directly of the unit of plasma collected. Comparison of the difference between the means of both groups was done using SPSS for Windows. It was found that the values obtained in serum were bigger than those obtained in plasma. Difference between the means of both groups was statistically significant (p 0.00). It is not advisable to use the obtained plasma of the plasmapheresis as analytic sample in this assay. |
| first_indexed | 2024-12-11T06:31:58Z |
| format | Article |
| id | doaj.art-4dca7a5983564fb6a4d385ad4a1a549a |
| institution | Directory Open Access Journal |
| issn | 1025-0298 |
| language | English |
| last_indexed | 2024-12-11T06:31:58Z |
| publishDate | 2015-08-01 |
| publisher | Finlay Ediciones |
| record_format | Article |
| series | VacciMonitor |
| spelling | doaj.art-4dca7a5983564fb6a4d385ad4a1a549a2022-12-22T01:17:28ZengFinlay EdicionesVacciMonitor1025-02982015-08-012415256Plasma as alternatively sample to quantify tetanus antitoxinAriel Menéndez-Barrios0Pedro Sánchez-Frenes1María de Jesús Sánchez-Bouza2Andrés Alemañi-Co3Sandra Gómez-Fonseca4Rodney Kidman Nieves-Armas51Banco de Sangre Provincial de Cienfuegos. Cienfuegos, Cuba1Banco de Sangre Provincial de Cienfuegos. Cienfuegos, Cuba2Universidad Médica de Cienfuegos “Raúl Dorticós Torrado”. Cienfuegos, CubaUniversidad Médica de Cienfuegos “Raúl Dorticós TorradoHospital General Universitario “Gustavo Alderegía Lima”. Cienfuegos, Cuba2Universidad Médica de Cienfuegos “Raúl Dorticós Torrado”. Cienfuegos, CubaTetanus antitoxin is quantified in Cuba at blood banks, from the serum of immunized donors, to produce aspecific human gamma globulin. A heterogeneous indirect immunoenzymatic assay is used, using the serum as analytical sample. The possible use of plasma obtained from plasmapheresis as alternative sample was evaluated in this research, to minimize the volume of total blood extracted to the donors. One hundred plasma donors who came to donate between October and November 2013 were selected by simple random sampling. Serum sample was obtained for extraction of 5 mL of blood, deposited in dry glass tube. While the other sample took 1.5 mL of plasma in a plastic tube with cover, at the end of the donation directly of the unit of plasma collected. Comparison of the difference between the means of both groups was done using SPSS for Windows. It was found that the values obtained in serum were bigger than those obtained in plasma. Difference between the means of both groups was statistically significant (p 0.00). It is not advisable to use the obtained plasma of the plasmapheresis as analytic sample in this assay.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100007&lng=es&nrm=isoimmunoassaysblood banksUMELISAtetanus antitoxin |
| spellingShingle | Ariel Menéndez-Barrios Pedro Sánchez-Frenes María de Jesús Sánchez-Bouza Andrés Alemañi-Co Sandra Gómez-Fonseca Rodney Kidman Nieves-Armas Plasma as alternatively sample to quantify tetanus antitoxin VacciMonitor immunoassays blood banks UMELISA tetanus antitoxin |
| title | Plasma as alternatively sample to quantify tetanus antitoxin |
| title_full | Plasma as alternatively sample to quantify tetanus antitoxin |
| title_fullStr | Plasma as alternatively sample to quantify tetanus antitoxin |
| title_full_unstemmed | Plasma as alternatively sample to quantify tetanus antitoxin |
| title_short | Plasma as alternatively sample to quantify tetanus antitoxin |
| title_sort | plasma as alternatively sample to quantify tetanus antitoxin |
| topic | immunoassays blood banks UMELISA tetanus antitoxin |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2015000100007&lng=es&nrm=iso |
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