Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton
Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbN...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-12-01
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| Series: | G3: Genes, Genomes, Genetics |
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| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.034512 |
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| author | Weina Wang Youlu Yuan Can Yang Shuaipeng Geng Quan Sun Lu Long Chaowei Cai Zongyan Chu Xin Liu Guanghao Wang Xiongming Du Chen Miao Xiao Zhang Yingfan Cai |
| author_facet | Weina Wang Youlu Yuan Can Yang Shuaipeng Geng Quan Sun Lu Long Chaowei Cai Zongyan Chu Xin Liu Guanghao Wang Xiongming Du Chen Miao Xiao Zhang Yingfan Cai |
| author_sort | Weina Wang |
| collection | DOAJ |
| description | Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants. |
| first_indexed | 2024-12-14T18:03:04Z |
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| id | doaj.art-4dcba525ede844f1929d47142a33a4b4 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-14T18:03:04Z |
| publishDate | 2016-12-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-4dcba525ede844f1929d47142a33a4b42022-12-21T22:52:25ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-12-016123951396110.1534/g3.116.03451216Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in CottonWeina WangYoulu YuanCan YangShuaipeng GengQuan SunLu LongChaowei CaiZongyan ChuXin LiuGuanghao WangXiongming DuChen MiaoXiao ZhangYingfan CaiElucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.http://g3journal.org/lookup/doi/10.1534/g3.116.034512NAC transcription factorVerticillium wiltVIGSbiotic and abiotic stresscotton |
| spellingShingle | Weina Wang Youlu Yuan Can Yang Shuaipeng Geng Quan Sun Lu Long Chaowei Cai Zongyan Chu Xin Liu Guanghao Wang Xiongming Du Chen Miao Xiao Zhang Yingfan Cai Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton G3: Genes, Genomes, Genetics NAC transcription factor Verticillium wilt VIGS biotic and abiotic stress cotton |
| title | Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton |
| title_full | Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton |
| title_fullStr | Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton |
| title_full_unstemmed | Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton |
| title_short | Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton |
| title_sort | characterization expression and functional analysis of a novel nac gene associated with resistance to verticillium wilt and abiotic stress in cotton |
| topic | NAC transcription factor Verticillium wilt VIGS biotic and abiotic stress cotton |
| url | http://g3journal.org/lookup/doi/10.1534/g3.116.034512 |
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