Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1273994/full |
| _version_ | 1797659506024382464 |
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| author | Mirunalini Ravichandran Danilo Maddalo |
| author_facet | Mirunalini Ravichandran Danilo Maddalo |
| author_sort | Mirunalini Ravichandran |
| collection | DOAJ |
| description | The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery. |
| first_indexed | 2024-03-11T18:16:04Z |
| format | Article |
| id | doaj.art-4dcbaf1613c149f480e17c26414af005 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-11T18:16:04Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-4dcbaf1613c149f480e17c26414af0052023-10-16T07:17:55ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-10-011410.3389/fgene.2023.12739941273994Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modelingMirunalini RavichandranDanilo MaddaloThe clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen to become the preferred gene-editing system due to its high efficiency, simplicity, and programmability at low costs. Furthermore, in the recent years, the CRISPR toolkit has been rapidly expanding, and the emerging advancements have shown tremendous potential in uncovering molecular mechanisms and new therapeutic strategies for human diseases. In this review, we provide our perspectives on the recent advancements in CRISPR technology and its impact on precision medicine, ranging from target identification, disease modeling, and diagnostics. We also discuss the impact of novel approaches such as epigenome, base, and prime editing on preclinical cancer drug discovery.https://www.frontiersin.org/articles/10.3389/fgene.2023.1273994/fullCRISPR/Cas9CRISPRa/iprecision oncologyepigenome editingbase editingprime editing |
| spellingShingle | Mirunalini Ravichandran Danilo Maddalo Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling Frontiers in Genetics CRISPR/Cas9 CRISPRa/i precision oncology epigenome editing base editing prime editing |
| title | Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling |
| title_full | Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling |
| title_fullStr | Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling |
| title_full_unstemmed | Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling |
| title_short | Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling |
| title_sort | applications of crispr cas9 for advancing precision medicine in oncology from target discovery to disease modeling |
| topic | CRISPR/Cas9 CRISPRa/i precision oncology epigenome editing base editing prime editing |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1273994/full |
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