Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.
Circulating nucleic acids are found in free form in body fluids and may serve as minimally invasive tools for cancer diagnosis and prognosis. Only a few studies have investigated the potential application of circulating mRNAs and microRNAs (miRNAs) in prostate cancer (PCa). The Cancer Genome Atlas (...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184094&type=printable |
| _version_ | 1826579060624457728 |
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| author | Marilesia Ferreira de Souza Hellen Kuasne Mateus de Camargo Barros-Filho Heloísa Lizotti Cilião Fabio Albuquerque Marchi Paulo Emilio Fuganti Alexandre Rossi Paschoal Silvia Regina Rogatto Ilce Mara de Syllos Cólus |
| author_facet | Marilesia Ferreira de Souza Hellen Kuasne Mateus de Camargo Barros-Filho Heloísa Lizotti Cilião Fabio Albuquerque Marchi Paulo Emilio Fuganti Alexandre Rossi Paschoal Silvia Regina Rogatto Ilce Mara de Syllos Cólus |
| author_sort | Marilesia Ferreira de Souza |
| collection | DOAJ |
| description | Circulating nucleic acids are found in free form in body fluids and may serve as minimally invasive tools for cancer diagnosis and prognosis. Only a few studies have investigated the potential application of circulating mRNAs and microRNAs (miRNAs) in prostate cancer (PCa). The Cancer Genome Atlas (TCGA) database was used for an in silico analysis to identify circulating mRNA and miRNA as potential markers of PCa. A total of 2,267 genes and 49 miRNAs were differentially expressed between normal and tumor samples. The prediction analyses of target genes and integrative analysis of mRNA and miRNA expression revealed eleven genes and eight miRNAs which were validated by RT-qPCR in plasma samples from 102 untreated PCa patients and 50 cancer-free individuals. Two genes, OR51E2 and SIM2, and two miRNAs, miR-200c and miR-200b, showed significant association with PCa. Expression levels of these transcripts distinguished PCa patients from controls (67% sensitivity and 75% specificity). PCa patients and controls with prostate-specific antigen (PSA) ≤ 4.0 ng/mL were discriminated based on OR51E2 and SIM2 expression levels. The miR-200c expression showed association with Gleason score and miR-200b, with bone metastasis, bilateral tumor, and PSA > 10.0 ng/mL. The combination of circulating mRNA and miRNA was useful for the diagnosis and prognosis of PCa. |
| first_indexed | 2024-12-13T21:37:23Z |
| format | Article |
| id | doaj.art-4dcf93b7f4c64c5db1234baa7deb25d6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T14:12:16Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4dcf93b7f4c64c5db1234baa7deb25d62025-02-27T05:35:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018409410.1371/journal.pone.0184094Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.Marilesia Ferreira de SouzaHellen KuasneMateus de Camargo Barros-FilhoHeloísa Lizotti CiliãoFabio Albuquerque MarchiPaulo Emilio FugantiAlexandre Rossi PaschoalSilvia Regina RogattoIlce Mara de Syllos CólusCirculating nucleic acids are found in free form in body fluids and may serve as minimally invasive tools for cancer diagnosis and prognosis. Only a few studies have investigated the potential application of circulating mRNAs and microRNAs (miRNAs) in prostate cancer (PCa). The Cancer Genome Atlas (TCGA) database was used for an in silico analysis to identify circulating mRNA and miRNA as potential markers of PCa. A total of 2,267 genes and 49 miRNAs were differentially expressed between normal and tumor samples. The prediction analyses of target genes and integrative analysis of mRNA and miRNA expression revealed eleven genes and eight miRNAs which were validated by RT-qPCR in plasma samples from 102 untreated PCa patients and 50 cancer-free individuals. Two genes, OR51E2 and SIM2, and two miRNAs, miR-200c and miR-200b, showed significant association with PCa. Expression levels of these transcripts distinguished PCa patients from controls (67% sensitivity and 75% specificity). PCa patients and controls with prostate-specific antigen (PSA) ≤ 4.0 ng/mL were discriminated based on OR51E2 and SIM2 expression levels. The miR-200c expression showed association with Gleason score and miR-200b, with bone metastasis, bilateral tumor, and PSA > 10.0 ng/mL. The combination of circulating mRNA and miRNA was useful for the diagnosis and prognosis of PCa.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184094&type=printable |
| spellingShingle | Marilesia Ferreira de Souza Hellen Kuasne Mateus de Camargo Barros-Filho Heloísa Lizotti Cilião Fabio Albuquerque Marchi Paulo Emilio Fuganti Alexandre Rossi Paschoal Silvia Regina Rogatto Ilce Mara de Syllos Cólus Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. PLoS ONE |
| title | Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. |
| title_full | Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. |
| title_fullStr | Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. |
| title_full_unstemmed | Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. |
| title_short | Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer. |
| title_sort | circulating mrnas and mirnas as candidate markers for the diagnosis and prognosis of prostate cancer |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184094&type=printable |
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