Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism
BackgroundIncreasing evidence has shown that elevated Thyroid stimulating hormone (TSH) levels are positively correlated with atherosclerosis (ATH) in patients with subclinical hypothyroidism (SCH). Some researchers found that the dysfunction of Endothelial Cells (ECs) in SCH plays an important role...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.981622/full |
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| author | Xuelin Yao Ying Wang Li Wang Mingfeng Cao Aifang Chen Xinhuan Zhang |
| author_facet | Xuelin Yao Ying Wang Li Wang Mingfeng Cao Aifang Chen Xinhuan Zhang |
| author_sort | Xuelin Yao |
| collection | DOAJ |
| description | BackgroundIncreasing evidence has shown that elevated Thyroid stimulating hormone (TSH) levels are positively correlated with atherosclerosis (ATH) in patients with subclinical hypothyroidism (SCH). Some researchers found that the dysfunction of Endothelial Cells (ECs) in SCH plays an important role in the pathogenesis of ATH in SCH, but the association remains controversial.ObjectivesTo determine the expression profiles of serum microRNAs critical to the function of Endothelial cells (ECs) may help reanalyze the possible mechanism underlying ATH in SCH and the association between ATH and SCH.MethodsWe used qRT-PCR to perform microRNA profiling and analysis in normal control subjects (NC), patients with SCH alone (SCH), patients with SCH and ATH (SCH+ATH), and patients with ATH without SCH (ATH).ResultsBoth miR-221-3p and miR-222-3p showed a decreasing expression trend between the SCH and SCH+ATH groups. In addition, miR-126-3p and miR-150-5p showed a stepwise decrease from the NC to SCH groups and then to the SCH+ATH or ATH group. miR-21-5p was unregulated in the SCH, SCH+ATH, and ATH groups. Furthermore, elevated levels of miR-21-5p in SCH+ATH group were higher than SCH and ATH group. No differences were found in the levels of miR-150, miR-126, miR-221 and miR-222 between the ATH and the SCH+ATH subjects.ConclusionsmiR-21-5p may be involved in the atherosclerosis process in patients with SCH (SCH and SCH+ATH groups). miR-150-5p may be sensitive risk markers for predicting endothelial dysfunction in patients with ATH (ATH and SCH+ATH groups). |
| first_indexed | 2024-04-12T05:05:56Z |
| format | Article |
| id | doaj.art-4dd4598de16b4342bdc7798f20dadf6e |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-12T05:05:56Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-4dd4598de16b4342bdc7798f20dadf6e2022-12-22T03:46:53ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-09-011310.3389/fendo.2022.981622981622Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidismXuelin Yao0Ying Wang1Li Wang2Mingfeng Cao3Aifang Chen4Xinhuan Zhang5Shandong Academy of Medical Sciences, Shandong First Medical University, Taian, ChinaDepartment of Endocrinology, the Second Affiliated Hospital of Shandong First Medical University, Taian, ChinaDepartment of Pharmacy, the Second Affiliated Hospital of Shandong First Medical University, Taian, ChinaDepartment of Endocrinology, the Second Affiliated Hospital of Shandong First Medical University, Taian, ChinaUltrasound Department, the Second Affiliated Hospital of Shandong First Medical University, Taian, ChinaDepartment of Endocrinology, the Second Affiliated Hospital of Shandong First Medical University, Taian, ChinaBackgroundIncreasing evidence has shown that elevated Thyroid stimulating hormone (TSH) levels are positively correlated with atherosclerosis (ATH) in patients with subclinical hypothyroidism (SCH). Some researchers found that the dysfunction of Endothelial Cells (ECs) in SCH plays an important role in the pathogenesis of ATH in SCH, but the association remains controversial.ObjectivesTo determine the expression profiles of serum microRNAs critical to the function of Endothelial cells (ECs) may help reanalyze the possible mechanism underlying ATH in SCH and the association between ATH and SCH.MethodsWe used qRT-PCR to perform microRNA profiling and analysis in normal control subjects (NC), patients with SCH alone (SCH), patients with SCH and ATH (SCH+ATH), and patients with ATH without SCH (ATH).ResultsBoth miR-221-3p and miR-222-3p showed a decreasing expression trend between the SCH and SCH+ATH groups. In addition, miR-126-3p and miR-150-5p showed a stepwise decrease from the NC to SCH groups and then to the SCH+ATH or ATH group. miR-21-5p was unregulated in the SCH, SCH+ATH, and ATH groups. Furthermore, elevated levels of miR-21-5p in SCH+ATH group were higher than SCH and ATH group. No differences were found in the levels of miR-150, miR-126, miR-221 and miR-222 between the ATH and the SCH+ATH subjects.ConclusionsmiR-21-5p may be involved in the atherosclerosis process in patients with SCH (SCH and SCH+ATH groups). miR-150-5p may be sensitive risk markers for predicting endothelial dysfunction in patients with ATH (ATH and SCH+ATH groups).https://www.frontiersin.org/articles/10.3389/fendo.2022.981622/fullsubclinical hypothyroidismatherosclerosisendothelial dysfunctioncirculating microRNAsdifferentially expressed miRNAs |
| spellingShingle | Xuelin Yao Ying Wang Li Wang Mingfeng Cao Aifang Chen Xinhuan Zhang Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism Frontiers in Endocrinology subclinical hypothyroidism atherosclerosis endothelial dysfunction circulating microRNAs differentially expressed miRNAs |
| title | Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism |
| title_full | Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism |
| title_fullStr | Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism |
| title_full_unstemmed | Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism |
| title_short | Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism |
| title_sort | expression patterns of serum micrornas related to endothelial dysfunction in patients with subclinical hypothyroidism |
| topic | subclinical hypothyroidism atherosclerosis endothelial dysfunction circulating microRNAs differentially expressed miRNAs |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.981622/full |
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