Apple juice evaluation: Qualitative analysis and microsatellite traceability

Qualitative and DNA analysis can be performed by taking a multidisciplinary approach to evaluate apple juices, the relevant values of which are a function of the origin, processing method and cultivar used. In detail, the aims of this study were to characterize apple juices through physiochemical an...

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Main Authors: Daniela Torello Marinoni, Paola Ruffa, Vera Pavese, Nicole Roberta Giuggioli
Format: Article
Language:English
Published: AIMS Press 2022-10-01
Series:AIMS Agriculture and Food
Subjects:
Online Access:https://www.aimspress.com/article/doi/10.3934/agrfood.2022050?viewType=HTML
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author Daniela Torello Marinoni
Paola Ruffa
Vera Pavese
Nicole Roberta Giuggioli
author_facet Daniela Torello Marinoni
Paola Ruffa
Vera Pavese
Nicole Roberta Giuggioli
author_sort Daniela Torello Marinoni
collection DOAJ
description Qualitative and DNA analysis can be performed by taking a multidisciplinary approach to evaluate apple juices, the relevant values of which are a function of the origin, processing method and cultivar used. In detail, the aims of this study were to characterize apple juices through physiochemical analysis, sensory analysis and DNA analysis to evaluate the efficiency of simple sequence repeat (SSR) markers for cultivar identification. Six apple juices made with cv Golden Delicious, cv Granny Smith and a mix of these cultivars from an e-commerce platform (Samples A and B), DISAFA (Samples C and D) and a local farm (Piedmont, Italy) (Samples E and F) were considered. Apple juices A, B, E and F (clarified and pasteurized) can be considered as being of high quality, while Samples C and D were unclarified, unpasteurized and made with apples purchased from a local store. Considering the qualitative analysis, it was observed that the cultivar of apple affected the parameters assessed. In the case of total phenolic compounds, the highest values were observed for juices made only with cv Granny Smith, suggesting how this cultivar contributes to maintaining these nutraceutical compounds more than cv Golden Delicious. Regarding DNA analysis, a limited number of markers, i.e., 4 and 3, respectively, for the apple juices originating from e-commerce and a local farm could successfully produce reproducible amplified fragments. These results can be related to the different procedures used in processing apple juices of different origins.
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spelling doaj.art-4dd6da715a214b7daf8937cf6fdf37ff2023-01-05T06:40:50ZengAIMS PressAIMS Agriculture and Food2471-20862022-10-017481983010.3934/agrfood.2022050Apple juice evaluation: Qualitative analysis and microsatellite traceabilityDaniela Torello Marinoni0Paola Ruffa 1Vera Pavese2Nicole Roberta Giuggioli3Department of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2,10095 Grugliasco, Torino, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2,10095 Grugliasco, Torino, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2,10095 Grugliasco, Torino, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2,10095 Grugliasco, Torino, ItalyQualitative and DNA analysis can be performed by taking a multidisciplinary approach to evaluate apple juices, the relevant values of which are a function of the origin, processing method and cultivar used. In detail, the aims of this study were to characterize apple juices through physiochemical analysis, sensory analysis and DNA analysis to evaluate the efficiency of simple sequence repeat (SSR) markers for cultivar identification. Six apple juices made with cv Golden Delicious, cv Granny Smith and a mix of these cultivars from an e-commerce platform (Samples A and B), DISAFA (Samples C and D) and a local farm (Piedmont, Italy) (Samples E and F) were considered. Apple juices A, B, E and F (clarified and pasteurized) can be considered as being of high quality, while Samples C and D were unclarified, unpasteurized and made with apples purchased from a local store. Considering the qualitative analysis, it was observed that the cultivar of apple affected the parameters assessed. In the case of total phenolic compounds, the highest values were observed for juices made only with cv Granny Smith, suggesting how this cultivar contributes to maintaining these nutraceutical compounds more than cv Golden Delicious. Regarding DNA analysis, a limited number of markers, i.e., 4 and 3, respectively, for the apple juices originating from e-commerce and a local farm could successfully produce reproducible amplified fragments. These results can be related to the different procedures used in processing apple juices of different origins.https://www.aimspress.com/article/doi/10.3934/agrfood.2022050?viewType=HTMLorigindnacultivarpolyphenolspanelist
spellingShingle Daniela Torello Marinoni
Paola Ruffa
Vera Pavese
Nicole Roberta Giuggioli
Apple juice evaluation: Qualitative analysis and microsatellite traceability
AIMS Agriculture and Food
origin
dna
cultivar
polyphenols
panelist
title Apple juice evaluation: Qualitative analysis and microsatellite traceability
title_full Apple juice evaluation: Qualitative analysis and microsatellite traceability
title_fullStr Apple juice evaluation: Qualitative analysis and microsatellite traceability
title_full_unstemmed Apple juice evaluation: Qualitative analysis and microsatellite traceability
title_short Apple juice evaluation: Qualitative analysis and microsatellite traceability
title_sort apple juice evaluation qualitative analysis and microsatellite traceability
topic origin
dna
cultivar
polyphenols
panelist
url https://www.aimspress.com/article/doi/10.3934/agrfood.2022050?viewType=HTML
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