Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China
Abstract Background Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody...
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| Format: | Article |
| Language: | English |
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BMC
2014-03-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1756-3305-7-138 |
| _version_ | 1797811677057515520 |
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| author | Yu-Chun Cai Jun-Fang Xu Peter Steinmann Shao-Hong Chen Yan-Hong Chu Li-Guang Tian Mu-Xin Chen Hao Li Yan Lu Ling-Ling Zhang Yang Zhou Jia-Xu Chen |
| author_facet | Yu-Chun Cai Jun-Fang Xu Peter Steinmann Shao-Hong Chen Yan-Hong Chu Li-Guang Tian Mu-Xin Chen Hao Li Yan Lu Ling-Ling Zhang Yang Zhou Jia-Xu Chen |
| author_sort | Yu-Chun Cai |
| collection | DOAJ |
| description | Abstract Background Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody and circulating antigen assays. Methods A total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in Hubei province were screened by serum examination with an indirect hemagglutination assay (IHA). The positive individuals (titer ≥20 in IHA) were reconfirmed by stool examination with the Kato-Katz method (three slides from a single stool specimen). Samples of good serum quality and a volume above 0.5 ml were selected for further testing with two immunodiagnostic antibody (DDIA and ELISA) and two antigen (ELISA) assays. Results The average antibody positive rate in the five villages was 12.7%, while the average parasitological prevalence was 1.50%; 25 of the 28 egg-positive samples were also circulating antigen-positive. Significant differences were observed between the prevalence according to the Kato-Katz method and all three immunodiagnostic antibody assays (P-value <0.0001). Similar differences were observed between the Kato-Katz method and the two immunodiagnostic antigen assays (P-value <0.0001) and between the antigen and antibody assays (P-value <0.0001). Conclusion Both circulating antibody and circulating antigen assays had acceptable performance characteristics. Immunodiagnostic techniques to detect circulating antigens have potential to be deployed for schistosomiasis japonica screening in the endemic areas. |
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| id | doaj.art-4dd85a46a4ec4d8095a493dd7b57a6c8 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:15Z |
| publishDate | 2014-03-01 |
| publisher | BMC |
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| series | Parasites & Vectors |
| spelling | doaj.art-4dd85a46a4ec4d8095a493dd7b57a6c82023-06-04T11:18:13ZengBMCParasites & Vectors1756-33052014-03-01711810.1186/1756-3305-7-138Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of ChinaYu-Chun Cai0Jun-Fang Xu1Peter Steinmann2Shao-Hong Chen3Yan-Hong Chu4Li-Guang Tian5Mu-Xin Chen6Hao Li7Yan Lu8Ling-Ling Zhang9Yang Zhou10Jia-Xu Chen11National Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionSchool of Medicine, Hubei University for NationalitiesDepartment of Epidemiology and Public Health, Swiss Tropical and Public Health InstituteNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionNational Institute of Parasitic Diseases, Chinese Center for Disease Control and PreventionAbstract Background Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody and circulating antigen assays. Methods A total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in Hubei province were screened by serum examination with an indirect hemagglutination assay (IHA). The positive individuals (titer ≥20 in IHA) were reconfirmed by stool examination with the Kato-Katz method (three slides from a single stool specimen). Samples of good serum quality and a volume above 0.5 ml were selected for further testing with two immunodiagnostic antibody (DDIA and ELISA) and two antigen (ELISA) assays. Results The average antibody positive rate in the five villages was 12.7%, while the average parasitological prevalence was 1.50%; 25 of the 28 egg-positive samples were also circulating antigen-positive. Significant differences were observed between the prevalence according to the Kato-Katz method and all three immunodiagnostic antibody assays (P-value <0.0001). Similar differences were observed between the Kato-Katz method and the two immunodiagnostic antigen assays (P-value <0.0001) and between the antigen and antibody assays (P-value <0.0001). Conclusion Both circulating antibody and circulating antigen assays had acceptable performance characteristics. Immunodiagnostic techniques to detect circulating antigens have potential to be deployed for schistosomiasis japonica screening in the endemic areas.https://doi.org/10.1186/1756-3305-7-138Schistosoma japonicumCirculating antibodyCirculating antigenChina |
| spellingShingle | Yu-Chun Cai Jun-Fang Xu Peter Steinmann Shao-Hong Chen Yan-Hong Chu Li-Guang Tian Mu-Xin Chen Hao Li Yan Lu Ling-Ling Zhang Yang Zhou Jia-Xu Chen Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China Parasites & Vectors Schistosoma japonicum Circulating antibody Circulating antigen China |
| title | Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China |
| title_full | Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China |
| title_fullStr | Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China |
| title_full_unstemmed | Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China |
| title_short | Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China |
| title_sort | field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of china |
| topic | Schistosoma japonicum Circulating antibody Circulating antigen China |
| url | https://doi.org/10.1186/1756-3305-7-138 |
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