Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest)
Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage &...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2009-09-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015 |
| _version_ | 1828257053778378752 |
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| author | Juliana Brandstetter Vilar Laryssa Silva de Andrade Kaio Ramos Leite Heleno Dias Ferreira Lee Chen Chen |
| author_facet | Juliana Brandstetter Vilar Laryssa Silva de Andrade Kaio Ramos Leite Heleno Dias Ferreira Lee Chen Chen |
| author_sort | Juliana Brandstetter Vilar |
| collection | DOAJ |
| description | Curatella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013), LB(1/2)(malt/amp), seeded into plates with the matches, and incubated for 24 h at 37 °C. After this period, the total number of colonies and the number of plaques were counted to evaluate C. americana cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of "lixeira" did not modify the survival of bacteria (p > 0.05), it caused a significant increase in prophage λ induction, especially at the higher concentrations (p<0.05). Therefore, we conclude that the ethanolic extract of C. americana stem bark did not present cytotoxic effect, but some genotoxic potential was observed.<br>Curatella americana L., comumente conhecida como "lixeira" no Brasil, é utilizada em medicina popular para tratamento de úlceras e inflamações. O presente trabalho teve como objetivo avaliar o potencial citotóxico e genotóxico do extrato etanólico das cascas de C. americana utilizando o Induteste SOS. Para avaliar a citotoxicidade da planta, depois de tratadas com diferentes concentrações do extrato, culturas de E. coli WP2s(λ) foram diluνdas em tampão M9 e semeadas em placas LB. Para avaliar a genotoxicidade da planta, a cepa lisogênica WP2s(λ) de E. coli foi tratada com diferentes concentrações do extrato. Em seguida, esta foi adicionada à cepa indicadora (RJF013) e ambas foram semeadas em placas em meio LB(1/2)(malt)(amp). Todas as culturas foram incubadas por 24 h a 37 °C. Posteriormente, o número total de colônias e o número de centros infecciosos foram computados para a avaliação da citotoxidade e da genotoxicidade desta planta, respectivamente. Os resultados mostraram que embora o extrato de C. americana não tenha modificado a sobrevivência bacteriana (p > 0,05), provocou aumento significativo (p < 0,05) na indução do profago λ, especialmente nas concentrações mais altas. Assim, concluiu-se que o extrato etanólico das cascas de C. americana não apresentou atividade citotóxica, mas foi observada ação genotóxica direta. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1984-8250 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T02:36:37Z |
| publishDate | 2009-09-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-4dd954556f5d4703bf3a45437f4e892e2022-12-22T03:06:22ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences1984-82502175-97902009-09-0145349149610.1590/S1984-82502009000300015Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest)Juliana Brandstetter VilarLaryssa Silva de AndradeKaio Ramos LeiteHeleno Dias FerreiraLee Chen ChenCuratella americana L., commonly known as "lixeira" in Brazil, has been used in folk medicine to treat ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain (RJF013), LB(1/2)(malt/amp), seeded into plates with the matches, and incubated for 24 h at 37 °C. After this period, the total number of colonies and the number of plaques were counted to evaluate C. americana cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of "lixeira" did not modify the survival of bacteria (p > 0.05), it caused a significant increase in prophage λ induction, especially at the higher concentrations (p<0.05). Therefore, we conclude that the ethanolic extract of C. americana stem bark did not present cytotoxic effect, but some genotoxic potential was observed.<br>Curatella americana L., comumente conhecida como "lixeira" no Brasil, é utilizada em medicina popular para tratamento de úlceras e inflamações. O presente trabalho teve como objetivo avaliar o potencial citotóxico e genotóxico do extrato etanólico das cascas de C. americana utilizando o Induteste SOS. Para avaliar a citotoxicidade da planta, depois de tratadas com diferentes concentrações do extrato, culturas de E. coli WP2s(λ) foram diluνdas em tampão M9 e semeadas em placas LB. Para avaliar a genotoxicidade da planta, a cepa lisogênica WP2s(λ) de E. coli foi tratada com diferentes concentrações do extrato. Em seguida, esta foi adicionada à cepa indicadora (RJF013) e ambas foram semeadas em placas em meio LB(1/2)(malt)(amp). Todas as culturas foram incubadas por 24 h a 37 °C. Posteriormente, o número total de colônias e o número de centros infecciosos foram computados para a avaliação da citotoxidade e da genotoxicidade desta planta, respectivamente. Os resultados mostraram que embora o extrato de C. americana não tenha modificado a sobrevivência bacteriana (p > 0,05), provocou aumento significativo (p < 0,05) na indução do profago λ, especialmente nas concentrações mais altas. Assim, concluiu-se que o extrato etanólico das cascas de C. americana não apresentou atividade citotóxica, mas foi observada ação genotóxica direta.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015Curatella americanaLixeiraCitotoxicidadeGenotoxicidadeIndução lisogênicaCuratella americanaLixeiraCytotoxicityGenotoxicityLysogenic induction |
| spellingShingle | Juliana Brandstetter Vilar Laryssa Silva de Andrade Kaio Ramos Leite Heleno Dias Ferreira Lee Chen Chen Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) Brazilian Journal of Pharmaceutical Sciences Curatella americana Lixeira Citotoxicidade Genotoxicidade Indução lisogênica Curatella americana Lixeira Cytotoxicity Genotoxicity Lysogenic induction |
| title | Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) |
| title_full | Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) |
| title_fullStr | Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) |
| title_full_unstemmed | Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) |
| title_short | Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americanaL.) λ using the prophage induction test (SOS inductest) |
| title_sort | assessment of genotoxicity and cytotoxicity of lixeira curatella americanal 955 using the prophage induction test sos inductest |
| topic | Curatella americana Lixeira Citotoxicidade Genotoxicidade Indução lisogênica Curatella americana Lixeira Cytotoxicity Genotoxicity Lysogenic induction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300015 |
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