Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers
<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 10pt;" class="MsoNormal"><span style="font-family: Calibri;"><span style="color: #211d1e; line-height: 115%; font-size: 10pt; mso-bi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2010-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/3611 |
| _version_ | 1818171631748513792 |
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| author | Beáta ÁBRAHÁM Ildikó MIKLÓSSY Erika KOVÁCS Éva TAMÁS Ildikó MÉSZÁROS Szabolcs SZILVESZTER Aurelia BREZEANU Szabolcs LÁNYI |
| author_facet | Beáta ÁBRAHÁM Ildikó MIKLÓSSY Erika KOVÁCS Éva TAMÁS Ildikó MÉSZÁROS Szabolcs SZILVESZTER Aurelia BREZEANU Szabolcs LÁNYI |
| author_sort | Beáta ÁBRAHÁM |
| collection | DOAJ |
| description | <span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 10pt;" class="MsoNormal"><span style="font-family: Calibri;"><span style="color: #211d1e; line-height: 115%; font-size: 10pt; mso-bidi-font-family: "Garamond Premier Pro";">The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain) <span style="mso-bidi-font-style: italic;">Pinus sylvestris </span>populations using molecular markers. Two of populations (Mohos and Luci) seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA). Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. <span style="mso-bidi-font-style: italic;">Pinus sylvestris </span>experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in <span style="mso-bidi-font-style: italic;">Pinus sylvestris.</span></span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span> |
| first_indexed | 2024-12-11T18:59:47Z |
| format | Article |
| id | doaj.art-4dd9cc066d054c418bb466bcc283ee3c |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-11T18:59:47Z |
| publishDate | 2010-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-4dd9cc066d054c418bb466bcc283ee3c2022-12-22T00:54:02ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642010-03-01211291324817Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD MarkersBeáta ÁBRAHÁM0Ildikó MIKLÓSSY1Erika KOVÁCSÉva TAMÁSIldikó MÉSZÁROS2Szabolcs SZILVESZTERAurelia BREZEANU3Szabolcs LÁNYI4Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.Institute of Biology Romanian Academy, Bucuresti, Spl. Independentei 296Sapientia University, Cluj Napoca, Department of Technical Sciences, Miercurea Ciuc Campus, 1 Liberty Str.<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 10pt;" class="MsoNormal"><span style="font-family: Calibri;"><span style="color: #211d1e; line-height: 115%; font-size: 10pt; mso-bidi-font-family: "Garamond Premier Pro";">The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain) <span style="mso-bidi-font-style: italic;">Pinus sylvestris </span>populations using molecular markers. Two of populations (Mohos and Luci) seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA). Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. <span style="mso-bidi-font-style: italic;">Pinus sylvestris </span>experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in <span style="mso-bidi-font-style: italic;">Pinus sylvestris.</span></span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span>http://notulaebiologicae.ro/index.php/nsb/article/view/3611 |
| spellingShingle | Beáta ÁBRAHÁM Ildikó MIKLÓSSY Erika KOVÁCS Éva TAMÁS Ildikó MÉSZÁROS Szabolcs SZILVESZTER Aurelia BREZEANU Szabolcs LÁNYI Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers Notulae Scientia Biologicae |
| title | Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers |
| title_full | Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers |
| title_fullStr | Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers |
| title_full_unstemmed | Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers |
| title_short | Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers |
| title_sort | genetic analysis of pinus sylvestris l and pinus sylvestris forma turfosa l using rapd markers |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/3611 |
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