Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck
Abstract Background A crucial role for the immune system has been proposed in the establishment and progression of head and neck squamous cell carcinoma (HNSCC). In this study, we investigated the cytokine and regulatory profiles of T cells in tumor draining lymph nodes (TDLNs) of patients with HNSC...
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| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-022-00530-3 |
| _version_ | 1811318117368856576 |
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| author | Marzieh Norouzian Fereshteh Mehdipour Mohammad Javad Ashraf Bijan Khademi Abbas Ghaderi |
| author_facet | Marzieh Norouzian Fereshteh Mehdipour Mohammad Javad Ashraf Bijan Khademi Abbas Ghaderi |
| author_sort | Marzieh Norouzian |
| collection | DOAJ |
| description | Abstract Background A crucial role for the immune system has been proposed in the establishment and progression of head and neck squamous cell carcinoma (HNSCC). In this study, we investigated the cytokine and regulatory profiles of T cells in tumor draining lymph nodes (TDLNs) of patients with HNSCC. Results The frequencies of CD4+TNF-α+ and CD4+TNF-αhi negatively were associated with poor prognostic factors such as LN involvement (P = 0.015 and P = 0.019, respectively), stage of the disease (P = 0.032 and P = 0.010, respectively) and tumor size (P = 0.026 and P = 0.032, respectively). Frequencies of CD8+IFN-γ+ and CD8+IFN-γ+ TNF-α+ T cells showed negative relationship with tumor grade (P = 0.035 and P = 0.043, respectively). While, the frequencies of CD4+IL-4+, CD8+IL-10+, CD8+IL-4+T cells were higher in advanced stages of the disease (P = 0.042, P = 0.041 and P = 0.030, respectively) and CD4+IFN-γ+TNF-α−, CD8+IL-4+ and CD8+IFN-γ+TNF-α− T cells were higher in patients with larger tumor size (P = 0.026 and P = 0.032, respectively). Negative associations were found between the frequencies of CD4+CD25+Foxp3+ and CD4+CD25+Foxp3+CD127low/− Treg cells and cancer stage (P = 0.015 and P = 0.059). Conclusion This study shed more lights on the changes in immune profile of T cells in TDLNs of HNSCC. Larger tumor size and/or LN involvement were associated with lower frequencies of CD4+TNF-α+, CD8+IFN-γ+ and CD8+IFN-γ+TNF-α+ but higher frequency of CD4+IL-4+ T cells. Moreover, Foxp3+Tregs correlated with good prognostic indicators. |
| first_indexed | 2024-04-13T12:20:26Z |
| format | Article |
| id | doaj.art-4ddb4ea36d5b4146bb28b7833344f622 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-04-13T12:20:26Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-4ddb4ea36d5b4146bb28b7833344f6222022-12-22T02:47:14ZengBMCBMC Immunology1471-21722022-11-0123111910.1186/s12865-022-00530-3Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neckMarzieh Norouzian0Fereshteh Mehdipour1Mohammad Javad Ashraf2Bijan Khademi3Abbas Ghaderi4Department of Laboratory Sciences, School of Allied Medical Sciences, Hormozgan University of Medical SciencesShiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical SciencesDepartment of Oral Pathology, School of Medicine, Shiraz University of Medical SciencesDepartment of Otolaryngology, School of Medicine, Shiraz University of Medical SciencesShiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical SciencesAbstract Background A crucial role for the immune system has been proposed in the establishment and progression of head and neck squamous cell carcinoma (HNSCC). In this study, we investigated the cytokine and regulatory profiles of T cells in tumor draining lymph nodes (TDLNs) of patients with HNSCC. Results The frequencies of CD4+TNF-α+ and CD4+TNF-αhi negatively were associated with poor prognostic factors such as LN involvement (P = 0.015 and P = 0.019, respectively), stage of the disease (P = 0.032 and P = 0.010, respectively) and tumor size (P = 0.026 and P = 0.032, respectively). Frequencies of CD8+IFN-γ+ and CD8+IFN-γ+ TNF-α+ T cells showed negative relationship with tumor grade (P = 0.035 and P = 0.043, respectively). While, the frequencies of CD4+IL-4+, CD8+IL-10+, CD8+IL-4+T cells were higher in advanced stages of the disease (P = 0.042, P = 0.041 and P = 0.030, respectively) and CD4+IFN-γ+TNF-α−, CD8+IL-4+ and CD8+IFN-γ+TNF-α− T cells were higher in patients with larger tumor size (P = 0.026 and P = 0.032, respectively). Negative associations were found between the frequencies of CD4+CD25+Foxp3+ and CD4+CD25+Foxp3+CD127low/− Treg cells and cancer stage (P = 0.015 and P = 0.059). Conclusion This study shed more lights on the changes in immune profile of T cells in TDLNs of HNSCC. Larger tumor size and/or LN involvement were associated with lower frequencies of CD4+TNF-α+, CD8+IFN-γ+ and CD8+IFN-γ+TNF-α+ but higher frequency of CD4+IL-4+ T cells. Moreover, Foxp3+Tregs correlated with good prognostic indicators.https://doi.org/10.1186/s12865-022-00530-3Head and neck squamous cell carcinomaTumor draining lymph nodesT lymphocytesRegulatory T cellsCytokine profileImmune profile |
| spellingShingle | Marzieh Norouzian Fereshteh Mehdipour Mohammad Javad Ashraf Bijan Khademi Abbas Ghaderi Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck BMC Immunology Head and neck squamous cell carcinoma Tumor draining lymph nodes T lymphocytes Regulatory T cells Cytokine profile Immune profile |
| title | Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| title_full | Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| title_fullStr | Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| title_full_unstemmed | Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| title_short | Regulatory and effector T cell subsets in tumor-draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| title_sort | regulatory and effector t cell subsets in tumor draining lymph nodes of patients with squamous cell carcinoma of head and neck |
| topic | Head and neck squamous cell carcinoma Tumor draining lymph nodes T lymphocytes Regulatory T cells Cytokine profile Immune profile |
| url | https://doi.org/10.1186/s12865-022-00530-3 |
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