Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease
ABSTRACT Objective To evaluate the induction of DNA damage in peripheral blood mononuclear cells of patients with sickle cell disease, SS and SC genotypes, treated with hydroxyurea. Methods The study subjects were divided into two groups: one group of 22 patients with sickle cell disease, SS and...
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400210&lng=en&tlng=en |
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| author | Emanuel Almeida Moreira de Oliveira Kenia de Assis Boy Ana Paula Pinho Santos Carla da Silva Machado Cibele Velloso-Rodrigues Pâmela Souza Almeida Silva Gerheim Leonardo Meneghin Mendonça |
| author_facet | Emanuel Almeida Moreira de Oliveira Kenia de Assis Boy Ana Paula Pinho Santos Carla da Silva Machado Cibele Velloso-Rodrigues Pâmela Souza Almeida Silva Gerheim Leonardo Meneghin Mendonça |
| author_sort | Emanuel Almeida Moreira de Oliveira |
| collection | DOAJ |
| description | ABSTRACT Objective To evaluate the induction of DNA damage in peripheral blood mononuclear cells of patients with sickle cell disease, SS and SC genotypes, treated with hydroxyurea. Methods The study subjects were divided into two groups: one group of 22 patients with sickle cell disease, SS and SC genotypes, treated with hydroxyurea, and a Control Group composed of 24 patients with sickle cell disease who were not treated with hydroxyurea. Peripheral blood samples were submitted to peripheral blood mononuclear cell isolation to assess genotoxicity by the cytokinesis-block micronucleus cytome assay, in which DNA damage biomarkers – micronuclei, nucleoplasmic bridges and nuclear buds - were counted. Results Patients with sickle cell disease treated with hydroxyurea had a mean age of 25.4 years, whereas patients with sickle cell disease not treated with hydroxyurea had a mean age of 17.6 years. The mean dose of hydroxyurea used by the patients was 12.8mg/kg/day, for a mean period of 44 months. The mean micronucleus frequency per 1,000 cells of 8.591±1.568 was observed in the Hydroxyurea Group and 10.040±1.003 in the Control Group. The mean frequency of nucleoplasmic bridges per 1,000 cells and nuclear buds per 1,000 cells for the hydroxyurea and Control Groups were 0.4545±0.1707 versus 0.5833±0.2078, and 0.8182±0.2430 versus 0.9583±0.1853, respectively. There was no statistically significant difference between groups. Conclusion In the study population, patients with sickle cell disease treated with the standard dose of hydroxyurea treatment did not show evidence of DNA damage induction. |
| first_indexed | 2024-04-13T16:22:51Z |
| format | Article |
| id | doaj.art-4ddc0f9cf0de48e59054aa5894029788 |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-04-13T16:22:51Z |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-4ddc0f9cf0de48e59054aa58940297882022-12-22T02:39:51ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-638517410.31744/einstein_journal/2019ao4742S1679-45082019000400210Evaluation of hydroxyurea genotoxicity in patients with sickle cell diseaseEmanuel Almeida Moreira de OliveiraKenia de Assis BoyAna Paula Pinho SantosCarla da Silva MachadoCibele Velloso-RodriguesPâmela Souza Almeida Silva GerheimLeonardo Meneghin MendonçaABSTRACT Objective To evaluate the induction of DNA damage in peripheral blood mononuclear cells of patients with sickle cell disease, SS and SC genotypes, treated with hydroxyurea. Methods The study subjects were divided into two groups: one group of 22 patients with sickle cell disease, SS and SC genotypes, treated with hydroxyurea, and a Control Group composed of 24 patients with sickle cell disease who were not treated with hydroxyurea. Peripheral blood samples were submitted to peripheral blood mononuclear cell isolation to assess genotoxicity by the cytokinesis-block micronucleus cytome assay, in which DNA damage biomarkers – micronuclei, nucleoplasmic bridges and nuclear buds - were counted. Results Patients with sickle cell disease treated with hydroxyurea had a mean age of 25.4 years, whereas patients with sickle cell disease not treated with hydroxyurea had a mean age of 17.6 years. The mean dose of hydroxyurea used by the patients was 12.8mg/kg/day, for a mean period of 44 months. The mean micronucleus frequency per 1,000 cells of 8.591±1.568 was observed in the Hydroxyurea Group and 10.040±1.003 in the Control Group. The mean frequency of nucleoplasmic bridges per 1,000 cells and nuclear buds per 1,000 cells for the hydroxyurea and Control Groups were 0.4545±0.1707 versus 0.5833±0.2078, and 0.8182±0.2430 versus 0.9583±0.1853, respectively. There was no statistically significant difference between groups. Conclusion In the study population, patients with sickle cell disease treated with the standard dose of hydroxyurea treatment did not show evidence of DNA damage induction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400210&lng=en&tlng=enAnemia falciformeHidroxiureiaGenotoxicidadeTestes de mutagenicidadeTestes para micronúcleos |
| spellingShingle | Emanuel Almeida Moreira de Oliveira Kenia de Assis Boy Ana Paula Pinho Santos Carla da Silva Machado Cibele Velloso-Rodrigues Pâmela Souza Almeida Silva Gerheim Leonardo Meneghin Mendonça Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease Einstein (São Paulo) Anemia falciforme Hidroxiureia Genotoxicidade Testes de mutagenicidade Testes para micronúcleos |
| title | Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| title_full | Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| title_fullStr | Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| title_full_unstemmed | Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| title_short | Evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| title_sort | evaluation of hydroxyurea genotoxicity in patients with sickle cell disease |
| topic | Anemia falciforme Hidroxiureia Genotoxicidade Testes de mutagenicidade Testes para micronúcleos |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082019000400210&lng=en&tlng=en |
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