Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes
Stems are more important to forage quality than leaves in alfalfa. To understand lignin formation at different stages in alfalfa, lignin distribution, anatomical characteristics and transcriptome profile were employed using two alfalfa cultivars. The results showed that the in vitro true digestibili...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/19/2601 |
| _version_ | 1797477380827119616 |
|---|---|
| author | Jierui Wu Xiaoyu Wang Ruxue Zhang Qingwen Fu Fang Tang Fengling Shi Buhe Temuer Zhiqiang Zhang |
| author_facet | Jierui Wu Xiaoyu Wang Ruxue Zhang Qingwen Fu Fang Tang Fengling Shi Buhe Temuer Zhiqiang Zhang |
| author_sort | Jierui Wu |
| collection | DOAJ |
| description | Stems are more important to forage quality than leaves in alfalfa. To understand lignin formation at different stages in alfalfa, lignin distribution, anatomical characteristics and transcriptome profile were employed using two alfalfa cultivars. The results showed that the in vitro true digestibility (IVTD) of stems in WL168 was significantly higher than that of Zhungeer, along with the significantly lower neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin contents. In addition, Zhungeer exhibited increased staining of the xylem areas in the stems of different developmental stages compared to WL168. Interestingly, the stems of WL168 appeared intracellular space from the stage 3, while Zhungeer did not. The comparative transcriptome analysis showed that a total of 1993 genes were differentially expressed in the stem between the cultivars, with a higher number of expressed genes in the stage 4. Of the differentially expressed genes, starch and sucrose metabolism as well as phenylpropanoid biosynthesis pathways were the most significantly enriched pathways. Furthermore, expression of genes involved in lignin biosynthesis such as <i>PAL</i>, <i>4CL</i>, <i>HCT</i>, <i>CAD</i>, <i>COMT</i> and <i>POD</i> coincides with the anatomic characteristics and lignin accumulation. These results may help elucidate the regulatory mechanisms of lignin biosynthesis and improve forage quality in alfalfa. |
| first_indexed | 2024-03-09T21:17:45Z |
| format | Article |
| id | doaj.art-4dde430f22df4f1ea14c687af0e186e4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:17:45Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4dde430f22df4f1ea14c687af0e186e42023-11-23T21:30:13ZengMDPI AGPlants2223-77472022-10-011119260110.3390/plants11192601Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa GenotypesJierui Wu0Xiaoyu Wang1Ruxue Zhang2Qingwen Fu3Fang Tang4Fengling Shi5Buhe Temuer6Zhiqiang Zhang7Key Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaKey Laboratory of Grassland Resources of the Ministry of Education, Technology Engineering Center of Drought and Cold-Resistant Grass Breeding in the North of the National Forestry and Grassland Administration, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010010, ChinaStems are more important to forage quality than leaves in alfalfa. To understand lignin formation at different stages in alfalfa, lignin distribution, anatomical characteristics and transcriptome profile were employed using two alfalfa cultivars. The results showed that the in vitro true digestibility (IVTD) of stems in WL168 was significantly higher than that of Zhungeer, along with the significantly lower neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin contents. In addition, Zhungeer exhibited increased staining of the xylem areas in the stems of different developmental stages compared to WL168. Interestingly, the stems of WL168 appeared intracellular space from the stage 3, while Zhungeer did not. The comparative transcriptome analysis showed that a total of 1993 genes were differentially expressed in the stem between the cultivars, with a higher number of expressed genes in the stage 4. Of the differentially expressed genes, starch and sucrose metabolism as well as phenylpropanoid biosynthesis pathways were the most significantly enriched pathways. Furthermore, expression of genes involved in lignin biosynthesis such as <i>PAL</i>, <i>4CL</i>, <i>HCT</i>, <i>CAD</i>, <i>COMT</i> and <i>POD</i> coincides with the anatomic characteristics and lignin accumulation. These results may help elucidate the regulatory mechanisms of lignin biosynthesis and improve forage quality in alfalfa.https://www.mdpi.com/2223-7747/11/19/2601<i>Medicago sativa</i> L.stemslignintranscriptomeanatomic |
| spellingShingle | Jierui Wu Xiaoyu Wang Ruxue Zhang Qingwen Fu Fang Tang Fengling Shi Buhe Temuer Zhiqiang Zhang Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes Plants <i>Medicago sativa</i> L. stems lignin transcriptome anatomic |
| title | Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes |
| title_full | Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes |
| title_fullStr | Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes |
| title_full_unstemmed | Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes |
| title_short | Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes |
| title_sort | comparative transcriptome and anatomic characteristics of stems in two alfalfa genotypes |
| topic | <i>Medicago sativa</i> L. stems lignin transcriptome anatomic |
| url | https://www.mdpi.com/2223-7747/11/19/2601 |
| work_keys_str_mv | AT jieruiwu comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT xiaoyuwang comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT ruxuezhang comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT qingwenfu comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT fangtang comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT fenglingshi comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT buhetemuer comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes AT zhiqiangzhang comparativetranscriptomeandanatomiccharacteristicsofstemsintwoalfalfagenotypes |