Evaluation of the level of alloantibodies and selected cytokines in kidney recipients
Introduction and objective Anti-HLA antibodies, especially anti-DSA, are believed to be potentially involved in acute and chronic rejection. New techniques, e.g. flow fluorimetry, can be an excellent supplement to the lymphocytotoxic tests currently used in pre-transplantation practice, and become a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2022-12-01
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| Series: | Journal of Pre-Clinical and Clinical Research |
| Subjects: | |
| Online Access: | https://www.jpccr.eu/Evaluation-of-the-level-of-alloantibodies-and-selected-cytokines-in-kidney-recipients,157283,0,2.html |
| _version_ | 1827989758971740160 |
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| author | Iwona Wojciechowska-Koszko Monika Nowosiad-Magda Barbara Krasnodębska-Szponder Paulina Roszkowska Michał Sławiński Paweł Kwiatkowski |
| author_facet | Iwona Wojciechowska-Koszko Monika Nowosiad-Magda Barbara Krasnodębska-Szponder Paulina Roszkowska Michał Sławiński Paweł Kwiatkowski |
| author_sort | Iwona Wojciechowska-Koszko |
| collection | DOAJ |
| description | Introduction and objective
Anti-HLA antibodies, especially anti-DSA, are believed to be potentially involved in acute
and chronic rejection. New techniques, e.g. flow fluorimetry, can be an excellent supplement to the lymphocytotoxic tests currently used in pre-transplantation practice, and become a tool for monitoring post-transplant immunisation. The aim of the study was to assess the level of cytokines and alloimmunisation in kidney recipients, with the histopathological evaluation of the transplanted kidney biopsy.
Material and methods
The study included 62 graft recipients six months after transplant. The level of anti-HLA antibodies
was assessed using the x-Map Luminex technique, and the level of cytokines (IFN-γ, IL-4, IL-10, IL-17) was determined using the ELISA test.
Results
Histopathological analysis showed that more than 45.2% of patients had changes in the biopsy material. Analysis of the level of alloimmunisation showed that in over 75.8%, the presence was detected of anti-HLA IgM class I antibodies, while anti-HLA IgM class II antibodies were found less often –17.8%. The level of anti-HLA IgG antibodies, depending on the type of the assessed class, respectively, was: class I – 43.5% and class II – 50%. More than half of the subjects also had anti-MICA antibodies. The level of analysed cytokines was low.
Conclusions
The results indicate significant alloimmunisation of kidney recipients, although they do not answer the
question whether these are antibodies that appeared de novo after transplantation, and whether anti-DSA antibodies were present among them. For this purpose, the diagnostics should be expanded to include anti-HLA monitoring in the pre- and post-transplant period, using screening tests and tests to identify their specificity. The protocol biopsy and examination of level cytokine can also be a helpful tool in post-transplant diagnosis. |
| first_indexed | 2024-04-10T00:25:12Z |
| format | Article |
| id | doaj.art-4ddf0bf7572843d29d67d685a0ab407a |
| institution | Directory Open Access Journal |
| issn | 1898-2395 1898-7516 |
| language | English |
| last_indexed | 2024-04-10T00:25:12Z |
| publishDate | 2022-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Journal of Pre-Clinical and Clinical Research |
| spelling | doaj.art-4ddf0bf7572843d29d67d685a0ab407a2023-03-15T10:41:34ZengInstitute of Rural HealthJournal of Pre-Clinical and Clinical Research1898-23951898-75162022-12-0116413113610.26444/jpccr/157283157283Evaluation of the level of alloantibodies and selected cytokines in kidney recipientsIwona Wojciechowska-Koszko0https://orcid.org/0000-0003-4887-4113Monika Nowosiad-Magda1https://orcid.org/0000-0002-9324-3532Barbara Krasnodębska-Szponder2https://orcid.org/0000-0003-2727-6667Paulina Roszkowska3https://orcid.org/0000-0002-2538-3808Michał Sławiński4https://orcid.org/0000-0001-8892-2015Paweł Kwiatkowski5https://orcid.org/0000-0003-0016-0541Department of Diagnostic Immunology, Pomeranian Medical University, Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University, Szczecin, PolandImmunology Laboratory Independent, Public Clinical Hospital No. 2, Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University, Szczecin, PolandDepartment of Laboratory Diagnostics, Public Clinical Hospital No. 2, Szczecin, PolandDepartment of Diagnostic Immunology, Pomeranian Medical University, Szczecin, PolandIntroduction and objective Anti-HLA antibodies, especially anti-DSA, are believed to be potentially involved in acute and chronic rejection. New techniques, e.g. flow fluorimetry, can be an excellent supplement to the lymphocytotoxic tests currently used in pre-transplantation practice, and become a tool for monitoring post-transplant immunisation. The aim of the study was to assess the level of cytokines and alloimmunisation in kidney recipients, with the histopathological evaluation of the transplanted kidney biopsy. Material and methods The study included 62 graft recipients six months after transplant. The level of anti-HLA antibodies was assessed using the x-Map Luminex technique, and the level of cytokines (IFN-γ, IL-4, IL-10, IL-17) was determined using the ELISA test. Results Histopathological analysis showed that more than 45.2% of patients had changes in the biopsy material. Analysis of the level of alloimmunisation showed that in over 75.8%, the presence was detected of anti-HLA IgM class I antibodies, while anti-HLA IgM class II antibodies were found less often –17.8%. The level of anti-HLA IgG antibodies, depending on the type of the assessed class, respectively, was: class I – 43.5% and class II – 50%. More than half of the subjects also had anti-MICA antibodies. The level of analysed cytokines was low. Conclusions The results indicate significant alloimmunisation of kidney recipients, although they do not answer the question whether these are antibodies that appeared de novo after transplantation, and whether anti-DSA antibodies were present among them. For this purpose, the diagnostics should be expanded to include anti-HLA monitoring in the pre- and post-transplant period, using screening tests and tests to identify their specificity. The protocol biopsy and examination of level cytokine can also be a helpful tool in post-transplant diagnosis.https://www.jpccr.eu/Evaluation-of-the-level-of-alloantibodies-and-selected-cytokines-in-kidney-recipients,157283,0,2.htmlcytokinesgraftanti-hlaanti-micabioptats |
| spellingShingle | Iwona Wojciechowska-Koszko Monika Nowosiad-Magda Barbara Krasnodębska-Szponder Paulina Roszkowska Michał Sławiński Paweł Kwiatkowski Evaluation of the level of alloantibodies and selected cytokines in kidney recipients Journal of Pre-Clinical and Clinical Research cytokines graft anti-hla anti-mica bioptats |
| title | Evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| title_full | Evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| title_fullStr | Evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| title_full_unstemmed | Evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| title_short | Evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| title_sort | evaluation of the level of alloantibodies and selected cytokines in kidney recipients |
| topic | cytokines graft anti-hla anti-mica bioptats |
| url | https://www.jpccr.eu/Evaluation-of-the-level-of-alloantibodies-and-selected-cytokines-in-kidney-recipients,157283,0,2.html |
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