Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication
Decellularization is a process to harvest the decellularized extra cellular matrix (dECM) that helps develop 3D scaffolds which mimic the native tissue composition. The decellularized tissues retain the structural and functional properties of the extracellular matrix (ECM) by an efficient decellular...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2024.2319893 |
| _version_ | 1826947730211078144 |
|---|---|
| author | Meghana Kasturi Kirthanashri S. Vasanthan |
| author_facet | Meghana Kasturi Kirthanashri S. Vasanthan |
| author_sort | Meghana Kasturi |
| collection | DOAJ |
| description | Decellularization is a process to harvest the decellularized extra cellular matrix (dECM) that helps develop 3D scaffolds which mimic the native tissue composition. The decellularized tissues retain the structural and functional properties of the extracellular matrix (ECM) by an efficient decellularization process that retains tissue-specific biochemical and biophysical cues for tissue regeneration. In this study, we report an injection-based decellularization method, without perfusion setup. This study also compares the efficiency of the proposed protocol in the two animal models viz rat and mice. This method harvests rat and mice liver dECM using ethylenediamine tetra acetic acid (EDTA) and sodium dodecyl sulphate (SDS) within 08 h and 02 h respectively and preserved significant amount of ECM proteins. We reported that the harvested mice decellularized extracellular matrix (mdECM) and rat decellularized extracellular matrix (rdECM) had significant reduction in their DNA content (∼97%) and retained structural architecture resembling their native tissue counterparts. The total protein content retained in mdECM was ∼39% while that retained in rdECM was ∼65%. It was also found that the sGAG (sulphated glycosaminoglycan) content showed a no List of Figures. |
| first_indexed | 2024-03-07T19:11:12Z |
| format | Article |
| id | doaj.art-4de12a9f937f4b269f9cfcb46e76185c |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2025-02-17T21:36:32Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-4de12a9f937f4b269f9cfcb46e76185c2024-12-06T10:48:00ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152117518510.1080/21691401.2024.2319893Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabricationMeghana Kasturi0Kirthanashri S. Vasanthan1Department of Mechanical Engineering, University of MI, Dearborn, USAManipal Centre for Biotherapeutics Research, Manipal Academy of Higher Education, Karnataka, IndiaDecellularization is a process to harvest the decellularized extra cellular matrix (dECM) that helps develop 3D scaffolds which mimic the native tissue composition. The decellularized tissues retain the structural and functional properties of the extracellular matrix (ECM) by an efficient decellularization process that retains tissue-specific biochemical and biophysical cues for tissue regeneration. In this study, we report an injection-based decellularization method, without perfusion setup. This study also compares the efficiency of the proposed protocol in the two animal models viz rat and mice. This method harvests rat and mice liver dECM using ethylenediamine tetra acetic acid (EDTA) and sodium dodecyl sulphate (SDS) within 08 h and 02 h respectively and preserved significant amount of ECM proteins. We reported that the harvested mice decellularized extracellular matrix (mdECM) and rat decellularized extracellular matrix (rdECM) had significant reduction in their DNA content (∼97%) and retained structural architecture resembling their native tissue counterparts. The total protein content retained in mdECM was ∼39% while that retained in rdECM was ∼65%. It was also found that the sGAG (sulphated glycosaminoglycan) content showed a no List of Figures.https://www.tandfonline.com/doi/10.1080/21691401.2024.2319893Decellularizationdecellularized extracellular matrix3D scaffoldsliver tissue engineering |
| spellingShingle | Meghana Kasturi Kirthanashri S. Vasanthan Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication Artificial Cells, Nanomedicine, and Biotechnology Decellularization decellularized extracellular matrix 3D scaffolds liver tissue engineering |
| title | Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication |
| title_full | Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication |
| title_fullStr | Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication |
| title_full_unstemmed | Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication |
| title_short | Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication |
| title_sort | harvesting decellularized liver extracellular matrix from rodents for 3d scaffold fabrication |
| topic | Decellularization decellularized extracellular matrix 3D scaffolds liver tissue engineering |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2024.2319893 |
| work_keys_str_mv | AT meghanakasturi harvestingdecellularizedliverextracellularmatrixfromrodentsfor3dscaffoldfabrication AT kirthanashrisvasanthan harvestingdecellularizedliverextracellularmatrixfromrodentsfor3dscaffoldfabrication |