DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms
Abstract Epigenetic influence plays a role in the association between exposure to air pollution and attention deficit hyperactivity disorder (ADHD); however, research regarding sulfur dioxide (SO2) is scarce. Herein, we investigate the associations between prenatal SO2 exposure and ADHD rating scale...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29843-y |
| _version_ | 1797864827526316032 |
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| author | Yoon-Jung Choi Jinwoo Cho Yun-Chul Hong Dong-wook Lee Sungji Moon Soo Jin Park Kyung-shin Lee Choong Ho Shin Young Ah Lee Bung-Nyun Kim Zachary Kaminsky Johanna Inhyang Kim Youn-Hee Lim |
| author_facet | Yoon-Jung Choi Jinwoo Cho Yun-Chul Hong Dong-wook Lee Sungji Moon Soo Jin Park Kyung-shin Lee Choong Ho Shin Young Ah Lee Bung-Nyun Kim Zachary Kaminsky Johanna Inhyang Kim Youn-Hee Lim |
| author_sort | Yoon-Jung Choi |
| collection | DOAJ |
| description | Abstract Epigenetic influence plays a role in the association between exposure to air pollution and attention deficit hyperactivity disorder (ADHD); however, research regarding sulfur dioxide (SO2) is scarce. Herein, we investigate the associations between prenatal SO2 exposure and ADHD rating scale (ARS) at ages 4, 6 and 8 years repeatedly in a mother–child cohort (n = 329). Whole blood samples were obtained at ages 2 and 6 years, and genome-wide DNA methylation (DNAm) was analyzed for 51 children using the Illumina Infinium HumanMethylation BeadChip. We analyzed the associations between prenatal SO2 exposure and DNAm levels at ages 2 and 6, and further investigated the association between the DNAm and ARS at ages 4, 6 and 8. Prenatal SO2 exposure was associated with ADHD symptoms. From candidate gene analysis, DNAm levels at the 6 CpGs at age 2 were associated with prenatal SO2 exposure levels. Of the 6 CpGs, cg07583420 (INS-IGF2) was persistently linked with ARS at ages 4, 6 and 8. Epigenome-wide analysis showed that DNAm at 6733 CpG sites were associated with prenatal SO2 exposure, of which 58 CpGs involved in Notch signalling pathway were further associated with ARS at age 4, 6 and 8 years, persistently. DNAm at age 6 was not associated with prenatal SO2 exposure. Changes in DNAm levels associated with prenatal SO2 exposure during early childhood are associated with increases in ARS in later childhood. |
| first_indexed | 2024-04-09T22:58:16Z |
| format | Article |
| id | doaj.art-4de3852e74a843c28261c883f7901470 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:58:16Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4de3852e74a843c28261c883f79014702023-03-22T11:08:07ZengNature PortfolioScientific Reports2045-23222023-03-0113111310.1038/s41598-023-29843-yDNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptomsYoon-Jung Choi0Jinwoo Cho1Yun-Chul Hong2Dong-wook Lee3Sungji Moon4Soo Jin Park5Kyung-shin Lee6Choong Ho Shin7Young Ah Lee8Bung-Nyun Kim9Zachary Kaminsky10Johanna Inhyang Kim11Youn-Hee Lim12National Cancer Center Graduate School of Cancer Science and PolicyDepartment of Statistics, University of PittsburghDepartment of Preventive Medicine, Seoul National University College of MedicineDepartment of Preventive Medicine, Seoul National University College of MedicineDepartment of Preventive Medicine, Seoul National University College of MedicineDepartment of Surgery, Wonkwang University Sanbon HospitalDepartment of Preventive Medicine, Seoul National University College of MedicineDepartment of Pediatrics, Seoul National University College of Medicine, Seoul National University Children’s HospitalDepartment of Pediatrics, Seoul National University College of Medicine, Seoul National University Children’s HospitalDivision of Children and Adolescent Psychiatry, Department of Psychiatry, Seoul National University HospitalInstitute of Mental Health Research, University of OttawaDepartment of Psychiatry, Hanyang University Medical CenterDepartment of Preventive Medicine, Seoul National University College of MedicineAbstract Epigenetic influence plays a role in the association between exposure to air pollution and attention deficit hyperactivity disorder (ADHD); however, research regarding sulfur dioxide (SO2) is scarce. Herein, we investigate the associations between prenatal SO2 exposure and ADHD rating scale (ARS) at ages 4, 6 and 8 years repeatedly in a mother–child cohort (n = 329). Whole blood samples were obtained at ages 2 and 6 years, and genome-wide DNA methylation (DNAm) was analyzed for 51 children using the Illumina Infinium HumanMethylation BeadChip. We analyzed the associations between prenatal SO2 exposure and DNAm levels at ages 2 and 6, and further investigated the association between the DNAm and ARS at ages 4, 6 and 8. Prenatal SO2 exposure was associated with ADHD symptoms. From candidate gene analysis, DNAm levels at the 6 CpGs at age 2 were associated with prenatal SO2 exposure levels. Of the 6 CpGs, cg07583420 (INS-IGF2) was persistently linked with ARS at ages 4, 6 and 8. Epigenome-wide analysis showed that DNAm at 6733 CpG sites were associated with prenatal SO2 exposure, of which 58 CpGs involved in Notch signalling pathway were further associated with ARS at age 4, 6 and 8 years, persistently. DNAm at age 6 was not associated with prenatal SO2 exposure. Changes in DNAm levels associated with prenatal SO2 exposure during early childhood are associated with increases in ARS in later childhood.https://doi.org/10.1038/s41598-023-29843-y |
| spellingShingle | Yoon-Jung Choi Jinwoo Cho Yun-Chul Hong Dong-wook Lee Sungji Moon Soo Jin Park Kyung-shin Lee Choong Ho Shin Young Ah Lee Bung-Nyun Kim Zachary Kaminsky Johanna Inhyang Kim Youn-Hee Lim DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms Scientific Reports |
| title | DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms |
| title_full | DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms |
| title_fullStr | DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms |
| title_full_unstemmed | DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms |
| title_short | DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms |
| title_sort | dna methylation is associated with prenatal exposure to sulfur dioxide and childhood attention deficit hyperactivity disorder symptoms |
| url | https://doi.org/10.1038/s41598-023-29843-y |
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