Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains
Abstract Objective Staphylococcus aureus and S. epidermidis as opportunistic pathogens, notable for their frequency and severity of infections are recognized as the most usual reasons for medical device-associated infections that strike hospitalized patients and also immunocompromised individuals. I...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4736-8 |
| _version_ | 1819277492972683264 |
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| author | Sanaz Amir Gholami Hamid Reza Goli Mohammad Reza Haghshenas Bahman Mirzaei |
| author_facet | Sanaz Amir Gholami Hamid Reza Goli Mohammad Reza Haghshenas Bahman Mirzaei |
| author_sort | Sanaz Amir Gholami |
| collection | DOAJ |
| description | Abstract Objective Staphylococcus aureus and S. epidermidis as opportunistic pathogens, notable for their frequency and severity of infections are recognized as the most usual reasons for medical device-associated infections that strike hospitalized patients and also immunocompromised individuals. In this study, the polysaccharide intercellular adhesion (PIA) and Glycerol teichoic acid) Gly-TA) as two major macromolecules in the biofilm formation process were purified under the native condition and their structure was analyzed by using colorimetric assays and Fourier Transform Infrared spectroscopy (FTIR). Afterward, the immune response of macromolecules and the mixture of them were assessed by measuring total IgG titers. Subsequently, biofilm inhibitory effects of raising antibodies to biofilm former S. aureus and S. epidermidis were evaluated. Results Obtained data were shown a significant rise in levels of antibodies in immunized mice with mentioned antibodies in comparison with the control group. According to the obtained findings, mentioned antibodies could eliminate S. aureus and S. epidermidis biofilm formation in vitro assays. This survey confirms the proposal that immunization of mice with a mixture of Gly-TA and PIA vaccine could be secure and protected against S. epidermidis and S. aureus infection. |
| first_indexed | 2024-12-23T23:56:59Z |
| format | Article |
| id | doaj.art-4de3b77425734269828d841478b2a0c6 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-23T23:56:59Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-4de3b77425734269828d841478b2a0c62022-12-21T17:25:14ZengBMCBMC Research Notes1756-05002019-10-011211610.1186/s13104-019-4736-8Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strainsSanaz Amir Gholami0Hamid Reza Goli1Mohammad Reza Haghshenas2Bahman Mirzaei3Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical ScienceDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical ScienceDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical ScienceDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical ScienceAbstract Objective Staphylococcus aureus and S. epidermidis as opportunistic pathogens, notable for their frequency and severity of infections are recognized as the most usual reasons for medical device-associated infections that strike hospitalized patients and also immunocompromised individuals. In this study, the polysaccharide intercellular adhesion (PIA) and Glycerol teichoic acid) Gly-TA) as two major macromolecules in the biofilm formation process were purified under the native condition and their structure was analyzed by using colorimetric assays and Fourier Transform Infrared spectroscopy (FTIR). Afterward, the immune response of macromolecules and the mixture of them were assessed by measuring total IgG titers. Subsequently, biofilm inhibitory effects of raising antibodies to biofilm former S. aureus and S. epidermidis were evaluated. Results Obtained data were shown a significant rise in levels of antibodies in immunized mice with mentioned antibodies in comparison with the control group. According to the obtained findings, mentioned antibodies could eliminate S. aureus and S. epidermidis biofilm formation in vitro assays. This survey confirms the proposal that immunization of mice with a mixture of Gly-TA and PIA vaccine could be secure and protected against S. epidermidis and S. aureus infection.http://link.springer.com/article/10.1186/s13104-019-4736-8PIA/Gly-TAMixtureBiofilm formationStaphylococcus aureusStaphylococcus epidermidis |
| spellingShingle | Sanaz Amir Gholami Hamid Reza Goli Mohammad Reza Haghshenas Bahman Mirzaei Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains BMC Research Notes PIA/Gly-TA Mixture Biofilm formation Staphylococcus aureus Staphylococcus epidermidis |
| title | Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains |
| title_full | Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains |
| title_fullStr | Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains |
| title_full_unstemmed | Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains |
| title_short | Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains |
| title_sort | evaluation of polysaccharide intercellular adhesion pia and glycerol teichoic acid gly ta arisen antibodies to prevention of biofilm formation in staphylococcus aureus and staphylococcus epidermidis strains |
| topic | PIA/Gly-TA Mixture Biofilm formation Staphylococcus aureus Staphylococcus epidermidis |
| url | http://link.springer.com/article/10.1186/s13104-019-4736-8 |
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