Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats
The effect of unmodified and functionalized <i>Spirulina platensis</i> biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirme...
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MDPI AG
2021-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/11/2992 |
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| author | Ludmila Rudi Inga Zinicovscaia Liliana Cepoi Tatiana Chiriac Alexandra Peshkova Anastasia Cepoi Dmitrii Grozdov |
| author_facet | Ludmila Rudi Inga Zinicovscaia Liliana Cepoi Tatiana Chiriac Alexandra Peshkova Anastasia Cepoi Dmitrii Grozdov |
| author_sort | Ludmila Rudi |
| collection | DOAJ |
| description | The effect of unmodified and functionalized <i>Spirulina platensis</i> biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process. |
| first_indexed | 2024-03-10T05:12:45Z |
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| id | doaj.art-4de6413363e0489e9e020f5361d85aa6 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T05:12:45Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4de6413363e0489e9e020f5361d85aa62023-11-23T00:41:33ZengMDPI AGNanomaterials2079-49912021-11-011111299210.3390/nano11112992Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on RatsLudmila Rudi0Inga Zinicovscaia1Liliana Cepoi2Tatiana Chiriac3Alexandra Peshkova4Anastasia Cepoi5Dmitrii Grozdov6Institute of Microbiology and Biotechnology, 1 Academiei Str., 2028 Chisinau, MoldovaJoint Institute for Nuclear Research, 6 Joliot-Curie Str., 141980 Dubna, RussiaInstitute of Microbiology and Biotechnology, 1 Academiei Str., 2028 Chisinau, MoldovaInstitute of Microbiology and Biotechnology, 1 Academiei Str., 2028 Chisinau, MoldovaJoint Institute for Nuclear Research, 6 Joliot-Curie Str., 141980 Dubna, RussiaInstitute of Microbiology and Biotechnology, 1 Academiei Str., 2028 Chisinau, MoldovaJoint Institute for Nuclear Research, 6 Joliot-Curie Str., 141980 Dubna, RussiaThe effect of unmodified and functionalized <i>Spirulina platensis</i> biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process.https://www.mdpi.com/2079-4991/11/11/2992<i>Spirulina platensis</i>silver nanoparticleshematological indicesbiochemical parametersneutron activation analysis |
| spellingShingle | Ludmila Rudi Inga Zinicovscaia Liliana Cepoi Tatiana Chiriac Alexandra Peshkova Anastasia Cepoi Dmitrii Grozdov Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats Nanomaterials <i>Spirulina platensis</i> silver nanoparticles hematological indices biochemical parameters neutron activation analysis |
| title | Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats |
| title_full | Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats |
| title_fullStr | Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats |
| title_full_unstemmed | Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats |
| title_short | Accumulation and Effect of Silver Nanoparticles Functionalized with <em>Spirulina platensis</em> on Rats |
| title_sort | accumulation and effect of silver nanoparticles functionalized with em spirulina platensis em on rats |
| topic | <i>Spirulina platensis</i> silver nanoparticles hematological indices biochemical parameters neutron activation analysis |
| url | https://www.mdpi.com/2079-4991/11/11/2992 |
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