3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration
The resection of malignant osteosarcoma often results in large segmental bone defects, and the residual cells can facilitate recurrence. Consequently, the treatment of osteosarcoma is a major challenge in clinical practice. The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly,...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-01-01
|
| Series: | Bioactive Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23002098 |
| _version_ | 1797752938570973184 |
|---|---|
| author | Hao Huang Lei Qiang Minjie Fan Yihao Liu Anchun Yang Dongbiao Chang Jinsheng Li Tong Sun Yiwei Wang Ruoyi Guo Hanjie Zhuang Xiangyu Li Tailin Guo Jinwu Wang Huan Tan Pengfei Zheng Jie Weng |
| author_facet | Hao Huang Lei Qiang Minjie Fan Yihao Liu Anchun Yang Dongbiao Chang Jinsheng Li Tong Sun Yiwei Wang Ruoyi Guo Hanjie Zhuang Xiangyu Li Tailin Guo Jinwu Wang Huan Tan Pengfei Zheng Jie Weng |
| author_sort | Hao Huang |
| collection | DOAJ |
| description | The resection of malignant osteosarcoma often results in large segmental bone defects, and the residual cells can facilitate recurrence. Consequently, the treatment of osteosarcoma is a major challenge in clinical practice. The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly, and repair the resultant bone defects as well as avoid bacterial infections. Herein, we fabricated a selenium/strontium/zinc-doped hydroxyapatite (Se/Sr/Zn-HA) powder by hydrothermal method, and then employed it with polycaprolactone (PCL) as ink to construct composite scaffolds through 3D printing, and finally introduced them in bone defect repair induced by malignant osteosarcoma. The resultant composite scaffolds integrated multiple functions involving anti-tumor, osteogenic, and antibacterial potentials, mainly attributed to the anti-tumor effects of SeO32−, osteogenic effects of Sr2+ and Zn2+, and antibacterial effects of SeO32− and Zn2+. In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells, differentiation of MSCs, and proliferation of MC3T3-E1 while showing excellent antibacterial properties. In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection, and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation. Summarily, the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment, bone defect repair, and post-operative anti-infection, which provided a great potential to be a facile therapeutic material for osteosarcoma resection. |
| first_indexed | 2024-03-12T17:11:10Z |
| format | Article |
| id | doaj.art-4de7a5a527764875938db3d43d7a1ef1 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-12T17:11:10Z |
| publishDate | 2024-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-4de7a5a527764875938db3d43d7a1ef12023-08-06T04:37:59ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-01-013118373D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regenerationHao Huang0Lei Qiang1Minjie Fan2Yihao Liu3Anchun Yang4Dongbiao Chang5Jinsheng Li6Tong Sun7Yiwei Wang8Ruoyi Guo9Hanjie Zhuang10Xiangyu Li11Tailin Guo12Jinwu Wang13Huan Tan14Pengfei Zheng15Jie Weng16Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR ChinaDepartment of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR ChinaDepartment of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaDepartment of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR ChinaDepartment of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR ChinaDepartment of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR China; School of Mechanical and Electrical Engineering, Henan University of Technology, Zhengzhou 450001, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR ChinaShanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, PR ChinaKey Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Corresponding author.Department of Orthopaedic Surgery, Children's Hospital of Nanjing Medical University, Nanjing 210008, PR China; Corresponding author.Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, College of Medicine, Southwest Jiaotong University, Chengdu, 610031, PR China; Corresponding author.The resection of malignant osteosarcoma often results in large segmental bone defects, and the residual cells can facilitate recurrence. Consequently, the treatment of osteosarcoma is a major challenge in clinical practice. The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly, and repair the resultant bone defects as well as avoid bacterial infections. Herein, we fabricated a selenium/strontium/zinc-doped hydroxyapatite (Se/Sr/Zn-HA) powder by hydrothermal method, and then employed it with polycaprolactone (PCL) as ink to construct composite scaffolds through 3D printing, and finally introduced them in bone defect repair induced by malignant osteosarcoma. The resultant composite scaffolds integrated multiple functions involving anti-tumor, osteogenic, and antibacterial potentials, mainly attributed to the anti-tumor effects of SeO32−, osteogenic effects of Sr2+ and Zn2+, and antibacterial effects of SeO32− and Zn2+. In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells, differentiation of MSCs, and proliferation of MC3T3-E1 while showing excellent antibacterial properties. In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection, and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation. Summarily, the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment, bone defect repair, and post-operative anti-infection, which provided a great potential to be a facile therapeutic material for osteosarcoma resection.http://www.sciencedirect.com/science/article/pii/S2452199X23002098Element-doped HA-PCL scaffolds3D printingOsteogenicOsteosarcomaAntibacterial |
| spellingShingle | Hao Huang Lei Qiang Minjie Fan Yihao Liu Anchun Yang Dongbiao Chang Jinsheng Li Tong Sun Yiwei Wang Ruoyi Guo Hanjie Zhuang Xiangyu Li Tailin Guo Jinwu Wang Huan Tan Pengfei Zheng Jie Weng 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration Bioactive Materials Element-doped HA-PCL scaffolds 3D printing Osteogenic Osteosarcoma Antibacterial |
| title | 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| title_full | 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| title_fullStr | 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| title_full_unstemmed | 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| title_short | 3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| title_sort | 3d printed tri element doped hydroxyapatite polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration |
| topic | Element-doped HA-PCL scaffolds 3D printing Osteogenic Osteosarcoma Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23002098 |
| work_keys_str_mv | AT haohuang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT leiqiang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT minjiefan 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT yihaoliu 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT anchunyang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT dongbiaochang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT jinshengli 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT tongsun 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT yiweiwang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT ruoyiguo 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT hanjiezhuang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT xiangyuli 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT tailinguo 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT jinwuwang 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT huantan 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT pengfeizheng 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration AT jieweng 3dprintedtrielementdopedhydroxyapatitepolycaprolactonecompositescaffoldswithantibacterialpotentialforosteosarcomatherapyandboneregeneration |