Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification
Over the years, bioinformatics tools have been used to identify functional genes. In the present study, bioinformatics analyses were conducted to explore the underlying molecular mechanisms of angiogenic factors in calcific aortic valve disease (CAVD). The raw gene expression profiles were from data...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1061077/full |
| _version_ | 1811170406892044288 |
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| author | XiangJin Kong XiangJin Kong LingWei Meng LingWei Meng KaiMing Wei KaiMing Wei Xin Lv Xin Lv ChuanZhen Liu ChuanZhen Liu FuShun Lin FuShun Lin XingHua Gu XingHua Gu |
| author_facet | XiangJin Kong XiangJin Kong LingWei Meng LingWei Meng KaiMing Wei KaiMing Wei Xin Lv Xin Lv ChuanZhen Liu ChuanZhen Liu FuShun Lin FuShun Lin XingHua Gu XingHua Gu |
| author_sort | XiangJin Kong |
| collection | DOAJ |
| description | Over the years, bioinformatics tools have been used to identify functional genes. In the present study, bioinformatics analyses were conducted to explore the underlying molecular mechanisms of angiogenic factors in calcific aortic valve disease (CAVD). The raw gene expression profiles were from datasets GSE153555, GSE83453, and GSE51472, and the angiogenesis-related gene set was from the Gene Set Enrichment Analysis database (GSEA). In this study, R was used to screen for differentially expressed genes (DEGs) and co-expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) Pathway enrichment analysis were performed on DEGs and validated in clinical samples. DEGs in CAVD were significantly enriched in numerous immune response pathways, inflammatory response pathways and angiogenesis-related pathways. Nine highly expressed angiogenesis-related genes were identified, of which secretogranin II (SCG2) was the most critical gene. MiRNA and transcription factors (TFs) networks were established centered on five DEGs, and zinc finger E-box binding homeobox 1 (ZEB1) was the most important transcription factor, verified by PCR, immunohistochemical staining and western blotting experiments. Overall, this study identified key genes and TFs that may be involved in the pathogenesis of CAVD and may have promising applications in the treatment of CAVD. |
| first_indexed | 2024-04-10T16:56:15Z |
| format | Article |
| id | doaj.art-4deb3f70e53940a5a376520c27551db4 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-10T16:56:15Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-4deb3f70e53940a5a376520c27551db42023-02-07T06:09:34ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-02-011010.3389/fcvm.2023.10610771061077Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcificationXiangJin Kong0XiangJin Kong1LingWei Meng2LingWei Meng3KaiMing Wei4KaiMing Wei5Xin Lv6Xin Lv7ChuanZhen Liu8ChuanZhen Liu9FuShun Lin10FuShun Lin11XingHua Gu12XingHua Gu13Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaQilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, ChinaDepartment of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaOver the years, bioinformatics tools have been used to identify functional genes. In the present study, bioinformatics analyses were conducted to explore the underlying molecular mechanisms of angiogenic factors in calcific aortic valve disease (CAVD). The raw gene expression profiles were from datasets GSE153555, GSE83453, and GSE51472, and the angiogenesis-related gene set was from the Gene Set Enrichment Analysis database (GSEA). In this study, R was used to screen for differentially expressed genes (DEGs) and co-expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) Pathway enrichment analysis were performed on DEGs and validated in clinical samples. DEGs in CAVD were significantly enriched in numerous immune response pathways, inflammatory response pathways and angiogenesis-related pathways. Nine highly expressed angiogenesis-related genes were identified, of which secretogranin II (SCG2) was the most critical gene. MiRNA and transcription factors (TFs) networks were established centered on five DEGs, and zinc finger E-box binding homeobox 1 (ZEB1) was the most important transcription factor, verified by PCR, immunohistochemical staining and western blotting experiments. Overall, this study identified key genes and TFs that may be involved in the pathogenesis of CAVD and may have promising applications in the treatment of CAVD.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1061077/fullcalcific aortic valve disease (CAVD)angiogenesis-related factorssecretogranin II (SCG2)zinc finger E-box binding homeobox 1 (ZEB1)miRNAtranscription factors (TFs) |
| spellingShingle | XiangJin Kong XiangJin Kong LingWei Meng LingWei Meng KaiMing Wei KaiMing Wei Xin Lv Xin Lv ChuanZhen Liu ChuanZhen Liu FuShun Lin FuShun Lin XingHua Gu XingHua Gu Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification Frontiers in Cardiovascular Medicine calcific aortic valve disease (CAVD) angiogenesis-related factors secretogranin II (SCG2) zinc finger E-box binding homeobox 1 (ZEB1) miRNA transcription factors (TFs) |
| title | Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification |
| title_full | Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification |
| title_fullStr | Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification |
| title_full_unstemmed | Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification |
| title_short | Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification |
| title_sort | exploration and validation of the influence of angiogenesis related factors in aortic valve calcification |
| topic | calcific aortic valve disease (CAVD) angiogenesis-related factors secretogranin II (SCG2) zinc finger E-box binding homeobox 1 (ZEB1) miRNA transcription factors (TFs) |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1061077/full |
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