The influence of platelet concentrate on the development of cattle embryos in аn in vitro system
There are many studies on the improvement of the conditions for cultivating bovine embryos in vitro. However, the issue of co-cultivation of platelets and cow embryos is understudied, although platelet activation leads to the release of a considerable amount of biologically active substances into th...
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| Format: | Article |
| Language: | English |
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Zhytomyr National Agroecological University
2022-11-01
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| Series: | Наукові горизонти |
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| Online Access: | https://sciencehorizon.com.ua/en/journals/tom-25-9-2022/vpliv-kontsentratu-trombotsitiv-na-rozvitok-embrioniv-velikoyi-rogatoyi-khudobi-u-sistemi-in-vitro |
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| author | Vitalii Kovpak Oksana Kovpak Serhii Derkach Yurii Masalovych Yelyzaveta Babiі |
| author_facet | Vitalii Kovpak Oksana Kovpak Serhii Derkach Yurii Masalovych Yelyzaveta Babiі |
| author_sort | Vitalii Kovpak |
| collection | DOAJ |
| description | There are many studies on the improvement of the conditions for cultivating bovine embryos in vitro. However, the issue of co-cultivation of platelets and cow embryos is understudied, although platelet activation leads to the release of a considerable amount of biologically active substances into the culture medium and their co-cultivation with embryos can positively affect the growth and development of the latter. Therefore, the purpose of this study was to investigate the effect of different platelet concentrations in the in vitro culture system on the development of cow embryos. Fertilized zygotes (total number 180) were divided into 5 groups: Group 1 – culture medium without platelet addition (control); Group 2 – medium for cultivation with the addition of 10×106 platelets/cm3 ; Group 3 – final concentration in the system is 20×106 platelets/cm3 ; Group 4 – 50×106 platelets/cm3 ; Group 5 – 100×106 platelets/cm3 . According to the results, it was found that co-cultivation of embryos with platelets in the in vitro system is effective. Thus, a correlation was found between improved embryo development indicators and an increase in platelet concentration. The optimal platelet concentration was 50×106 /cm3 , which allowed obtaining a 13.9% higher level of blastulation, 15.7% higher average embryo size, and 2.5% higher average number of cells in the blastocyst compared to the control. At the same time, the platelet concentration of 100×106 /cm3 led to a significant decrease in the indicators under study, compared with the group without platelets. Therefore, co-cultivation of cow embryos with platelets is advisable since it allows improving the development indicators of cow embryos. The data analysed and presented in this paper will increase the efficiency of cultivation of bovine embryos for both scientific and industrial purposes |
| first_indexed | 2024-03-13T00:53:05Z |
| format | Article |
| id | doaj.art-4deba08449ef44ec9a500dc497235a71 |
| institution | Directory Open Access Journal |
| issn | 2663-2144 |
| language | English |
| last_indexed | 2024-03-13T00:53:05Z |
| publishDate | 2022-11-01 |
| publisher | Zhytomyr National Agroecological University |
| record_format | Article |
| series | Наукові горизонти |
| spelling | doaj.art-4deba08449ef44ec9a500dc497235a712023-07-07T09:48:10ZengZhytomyr National Agroecological UniversityНаукові горизонти2663-21442022-11-0125991810.48077/scihor.25(9).2022.9-18The influence of platelet concentrate on the development of cattle embryos in аn in vitro systemVitalii Kovpak0Oksana Kovpak1Serhii Derkach2Yurii Masalovych3Yelyzaveta Babiі4National University of Life and Environmental Sciences of Ukraine 03041, 15 Heroiv Oborony Str., Kyiv, Ukraine LLC “BioTexCom” 04107, 2/6 Otto Shmidta Str., Kyiv, UkraineNational University of Life and Environmental Sciences of Ukraine 03041, 15 Heroiv Oborony Str., Kyiv, Ukraine National University of Life and Environmental Sciences of Ukraine 03041, 15 Heroiv Oborony Str., Kyiv, Ukraine National University of Life and Environmental Sciences of Ukraine 03041, 15 Heroiv Oborony Str., Kyiv, UkraineThere are many studies on the improvement of the conditions for cultivating bovine embryos in vitro. However, the issue of co-cultivation of platelets and cow embryos is understudied, although platelet activation leads to the release of a considerable amount of biologically active substances into the culture medium and their co-cultivation with embryos can positively affect the growth and development of the latter. Therefore, the purpose of this study was to investigate the effect of different platelet concentrations in the in vitro culture system on the development of cow embryos. Fertilized zygotes (total number 180) were divided into 5 groups: Group 1 – culture medium without platelet addition (control); Group 2 – medium for cultivation with the addition of 10×106 platelets/cm3 ; Group 3 – final concentration in the system is 20×106 platelets/cm3 ; Group 4 – 50×106 platelets/cm3 ; Group 5 – 100×106 platelets/cm3 . According to the results, it was found that co-cultivation of embryos with platelets in the in vitro system is effective. Thus, a correlation was found between improved embryo development indicators and an increase in platelet concentration. The optimal platelet concentration was 50×106 /cm3 , which allowed obtaining a 13.9% higher level of blastulation, 15.7% higher average embryo size, and 2.5% higher average number of cells in the blastocyst compared to the control. At the same time, the platelet concentration of 100×106 /cm3 led to a significant decrease in the indicators under study, compared with the group without platelets. Therefore, co-cultivation of cow embryos with platelets is advisable since it allows improving the development indicators of cow embryos. The data analysed and presented in this paper will increase the efficiency of cultivation of bovine embryos for both scientific and industrial purposeshttps://sciencehorizon.com.ua/en/journals/tom-25-9-2022/vpliv-kontsentratu-trombotsitiv-na-rozvitok-embrioniv-velikoyi-rogatoyi-khudobi-u-sistemi-in-vitrobiotechnology of ruminant reproductiongrowth factordivisionblastocyst |
| spellingShingle | Vitalii Kovpak Oksana Kovpak Serhii Derkach Yurii Masalovych Yelyzaveta Babiі The influence of platelet concentrate on the development of cattle embryos in аn in vitro system Наукові горизонти biotechnology of ruminant reproduction growth factor division blastocyst |
| title | The influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| title_full | The influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| title_fullStr | The influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| title_full_unstemmed | The influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| title_short | The influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| title_sort | influence of platelet concentrate on the development of cattle embryos in аn in vitro system |
| topic | biotechnology of ruminant reproduction growth factor division blastocyst |
| url | https://sciencehorizon.com.ua/en/journals/tom-25-9-2022/vpliv-kontsentratu-trombotsitiv-na-rozvitok-embrioniv-velikoyi-rogatoyi-khudobi-u-sistemi-in-vitro |
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