The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104
Introduction: Rotaviruses are the main cause of gastroenteritis in children under five years old. At this moment is unknown how these viruses entry to host cell. The heat shock cognate protein HSC70 has been involved in rotavirus cell entry. However, the relationship between rotavirus proteins and H...
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Colombia
2006-01-01
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| Series: | Revista de la Facultad de Medicina |
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/revfacmed/article/view/23095 |
| _version_ | 1818259328015007744 |
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| author | Luz Stella Rodríguez Carlos Arturo Guerrero Fonseca |
| author_facet | Luz Stella Rodríguez Carlos Arturo Guerrero Fonseca |
| author_sort | Luz Stella Rodríguez |
| collection | DOAJ |
| description | Introduction: Rotaviruses are the main cause of gastroenteritis in children under five years old. At this moment is unknown how these viruses entry to host cell. The heat shock cognate protein HSC70 has been involved in rotavirus cell entry. However, the relationship between rotavirus proteins and HSC70 has not been established.
Objectives: the purpose of this work was to determine wich rotaviruses proteins interact with HSC70.
Material and methods: coimmunoprecipitation assays were done on a rotavirus infected supernatant and recombinant viral proteins. These assays were revealed by SDS-PAGE and Western blot using policlonal antibodys against rotavirus structural proteins.
Results: data show that viral protein VP6 coimmunoprecipated with HSC70 in MA104 cells during infection. Recombinant vaccinia of VP6 produced in MA104 cells also coimmunoprecipitated with HSC70.
Conclusions: these results suggest the interaction between VP6 and HSC70 during rotavirus infection. Furthermore, this union may be independent of other rotaviruses proteins. |
| first_indexed | 2024-12-12T18:13:41Z |
| format | Article |
| id | doaj.art-4decd64a63154a72b53d9125cf44a57f |
| institution | Directory Open Access Journal |
| issn | 0120-0011 2357-3848 |
| language | English |
| last_indexed | 2024-12-12T18:13:41Z |
| publishDate | 2006-01-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista de la Facultad de Medicina |
| spelling | doaj.art-4decd64a63154a72b53d9125cf44a57f2022-12-22T00:16:19ZengUniversidad Nacional de ColombiaRevista de la Facultad de Medicina0120-00112357-38482006-01-01541243120553The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104Luz Stella Rodríguez0Carlos Arturo Guerrero Fonseca1Química, Estudiante de Maestría en Bioquímica, Facultad de Medicina, Universidad Nacional de Colombia, BogotáProfesor Asociado, Laboratorio de Biología Molecular y Celular de virus, Unidad de Bioquímica. Facultad de Medicina, Universidad Nacional de Colombia, Bogotá.Introduction: Rotaviruses are the main cause of gastroenteritis in children under five years old. At this moment is unknown how these viruses entry to host cell. The heat shock cognate protein HSC70 has been involved in rotavirus cell entry. However, the relationship between rotavirus proteins and HSC70 has not been established. Objectives: the purpose of this work was to determine wich rotaviruses proteins interact with HSC70. Material and methods: coimmunoprecipitation assays were done on a rotavirus infected supernatant and recombinant viral proteins. These assays were revealed by SDS-PAGE and Western blot using policlonal antibodys against rotavirus structural proteins. Results: data show that viral protein VP6 coimmunoprecipated with HSC70 in MA104 cells during infection. Recombinant vaccinia of VP6 produced in MA104 cells also coimmunoprecipitated with HSC70. Conclusions: these results suggest the interaction between VP6 and HSC70 during rotavirus infection. Furthermore, this union may be independent of other rotaviruses proteins.https://revistas.unal.edu.co/index.php/revfacmed/article/view/23095infección por rotavirusvacciniareoviridaerhesus (macaca mulatta). |
| spellingShingle | Luz Stella Rodríguez Carlos Arturo Guerrero Fonseca The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 Revista de la Facultad de Medicina infección por rotavirus vaccinia reoviridae rhesus (macaca mulatta). |
| title | The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 |
| title_full | The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 |
| title_fullStr | The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 |
| title_full_unstemmed | The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 |
| title_short | The viral protein VP6 of rotavirus coinmunoprecipated with HSC70 in cels MA104 |
| title_sort | viral protein vp6 of rotavirus coinmunoprecipated with hsc70 in cels ma104 |
| topic | infección por rotavirus vaccinia reoviridae rhesus (macaca mulatta). |
| url | https://revistas.unal.edu.co/index.php/revfacmed/article/view/23095 |
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