The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites.
Parasites causing malaria need to migrate in order to penetrate tissue barriers and enter host cells. Here we show that the actin filament-binding protein coronin regulates gliding motility in Plasmodium berghei sporozoites, the highly motile forms of a rodent malaria-causing parasite transmitted by...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-07-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4943629?pdf=render |
| _version_ | 1818512662035693568 |
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| author | Kartik S Bane Simone Lepper Jessica Kehrer Julia M Sattler Mirko Singer Miriam Reinig Dennis Klug Kirsten Heiss Jake Baum Ann-Kristin Mueller Friedrich Frischknecht |
| author_facet | Kartik S Bane Simone Lepper Jessica Kehrer Julia M Sattler Mirko Singer Miriam Reinig Dennis Klug Kirsten Heiss Jake Baum Ann-Kristin Mueller Friedrich Frischknecht |
| author_sort | Kartik S Bane |
| collection | DOAJ |
| description | Parasites causing malaria need to migrate in order to penetrate tissue barriers and enter host cells. Here we show that the actin filament-binding protein coronin regulates gliding motility in Plasmodium berghei sporozoites, the highly motile forms of a rodent malaria-causing parasite transmitted by mosquitoes. Parasites lacking coronin show motility defects that impair colonization of the mosquito salivary glands but not migration in the skin, yet result in decreased transmission efficiency. In non-motile sporozoites low calcium concentrations mediate actin-independent coronin localization to the periphery. Engagement of extracellular ligands triggers an intracellular calcium release followed by the actin-dependent relocalization of coronin to the rear and initiation of motility. Mutational analysis and imaging suggest that coronin organizes actin filaments for productive motility. Using coronin-mCherry as a marker for the presence of actin filaments we found that protein kinase A contributes to actin filament disassembly. We finally speculate that calcium and cAMP-mediated signaling regulate a switch from rapid parasite motility to host cell invasion by differentially influencing actin dynamics. |
| first_indexed | 2024-12-10T23:50:00Z |
| format | Article |
| id | doaj.art-4dedf6d7420c4c4fafc10a887f9ad6ff |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-10T23:50:00Z |
| publishDate | 2016-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-4dedf6d7420c4c4fafc10a887f9ad6ff2022-12-22T01:28:47ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-07-01127e100571010.1371/journal.ppat.1005710The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites.Kartik S BaneSimone LepperJessica KehrerJulia M SattlerMirko SingerMiriam ReinigDennis KlugKirsten HeissJake BaumAnn-Kristin MuellerFriedrich FrischknechtParasites causing malaria need to migrate in order to penetrate tissue barriers and enter host cells. Here we show that the actin filament-binding protein coronin regulates gliding motility in Plasmodium berghei sporozoites, the highly motile forms of a rodent malaria-causing parasite transmitted by mosquitoes. Parasites lacking coronin show motility defects that impair colonization of the mosquito salivary glands but not migration in the skin, yet result in decreased transmission efficiency. In non-motile sporozoites low calcium concentrations mediate actin-independent coronin localization to the periphery. Engagement of extracellular ligands triggers an intracellular calcium release followed by the actin-dependent relocalization of coronin to the rear and initiation of motility. Mutational analysis and imaging suggest that coronin organizes actin filaments for productive motility. Using coronin-mCherry as a marker for the presence of actin filaments we found that protein kinase A contributes to actin filament disassembly. We finally speculate that calcium and cAMP-mediated signaling regulate a switch from rapid parasite motility to host cell invasion by differentially influencing actin dynamics.http://europepmc.org/articles/PMC4943629?pdf=render |
| spellingShingle | Kartik S Bane Simone Lepper Jessica Kehrer Julia M Sattler Mirko Singer Miriam Reinig Dennis Klug Kirsten Heiss Jake Baum Ann-Kristin Mueller Friedrich Frischknecht The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. PLoS Pathogens |
| title | The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. |
| title_full | The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. |
| title_fullStr | The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. |
| title_full_unstemmed | The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. |
| title_short | The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites. |
| title_sort | actin filament binding protein coronin regulates motility in plasmodium sporozoites |
| url | http://europepmc.org/articles/PMC4943629?pdf=render |
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