First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution
Background: Guidelines recommend ABO-identical platelet (PLT) transfusions. Hemolytic reactions after a minor ABO-incompatible PLT transfusion have escalated due to single-donor platelets (SDP) containing ABO-incompatible plasma. Avoiding such events by examining titers or performing plasma reductio...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=10;epage=16;aulast=Jain |
| _version_ | 1818552118463692800 |
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| author | Puneet Jain Anita Tendulkar Abhaykumar Gupta |
| author_facet | Puneet Jain Anita Tendulkar Abhaykumar Gupta |
| author_sort | Puneet Jain |
| collection | DOAJ |
| description | Background: Guidelines recommend ABO-identical platelet (PLT) transfusions. Hemolytic reactions after a minor ABO-incompatible PLT transfusion have escalated due to single-donor platelets (SDP) containing ABO-incompatible plasma. Avoiding such events by examining titers or performing plasma reduction is cumbersome. The introduction of platelet additive solutions (PAS) has enabled to reduce these reactions by avoiding passive transfer of isoagglutinin. Our aim was to study antibody titers (anti-A, anti-B) in “O” SDP by adding PAS at source and the quality parameters with reference to viability, morphology, and metabolism.
Materials And Methods: Group “O” SDP (n = 50) were prepared on a standard cell separator. PAS in a ratio of 70:30 (PAS: plasma) was added at source under sterile conditions (study arm). The units were studied on day of collection (day 0) and day 4 and compared with SDP containing 100% plasma (control arm). A titer study was performed after PAS addition.
Results: In the study group, the median antibody titers (anti-A, anti-B) reduced from 128 to16, post-PAS addition (P < 0.001). Morphology scores were superior in PAS platelet concentrates (P < 0.001). Metabolic parameters pO2and pCO2were similar in the two arms signifying good unit storage and stable oxygen consumption (P > 0.05). Lactate levels, glucose consumption rate, and lactate production rates were significantly low in study arm showing the advantage of PAS.
Conclusion: O group SDPs can be prepared with PAS and the beneficial effects were significant with respect to antibody titers. Quality parameters were well maintained. Availability of PAS units has benefitted patients. |
| first_indexed | 2024-12-12T09:09:01Z |
| format | Article |
| id | doaj.art-4df066864c2b41ad821c9f900764c4d7 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-12-12T09:09:01Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-4df066864c2b41ad821c9f900764c4d72022-12-22T00:29:34ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652018-01-01121101610.4103/ajts.AJTS_2_17First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solutionPuneet JainAnita TendulkarAbhaykumar GuptaBackground: Guidelines recommend ABO-identical platelet (PLT) transfusions. Hemolytic reactions after a minor ABO-incompatible PLT transfusion have escalated due to single-donor platelets (SDP) containing ABO-incompatible plasma. Avoiding such events by examining titers or performing plasma reduction is cumbersome. The introduction of platelet additive solutions (PAS) has enabled to reduce these reactions by avoiding passive transfer of isoagglutinin. Our aim was to study antibody titers (anti-A, anti-B) in “O” SDP by adding PAS at source and the quality parameters with reference to viability, morphology, and metabolism. Materials And Methods: Group “O” SDP (n = 50) were prepared on a standard cell separator. PAS in a ratio of 70:30 (PAS: plasma) was added at source under sterile conditions (study arm). The units were studied on day of collection (day 0) and day 4 and compared with SDP containing 100% plasma (control arm). A titer study was performed after PAS addition. Results: In the study group, the median antibody titers (anti-A, anti-B) reduced from 128 to16, post-PAS addition (P < 0.001). Morphology scores were superior in PAS platelet concentrates (P < 0.001). Metabolic parameters pO2and pCO2were similar in the two arms signifying good unit storage and stable oxygen consumption (P > 0.05). Lactate levels, glucose consumption rate, and lactate production rates were significantly low in study arm showing the advantage of PAS. Conclusion: O group SDPs can be prepared with PAS and the beneficial effects were significant with respect to antibody titers. Quality parameters were well maintained. Availability of PAS units has benefitted patients.http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=10;epage=16;aulast=JainGroup O plateletslow titerplatelet additive solutionsquality parameterssingle-donor platelets |
| spellingShingle | Puneet Jain Anita Tendulkar Abhaykumar Gupta First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution Asian Journal of Transfusion Science Group O platelets low titer platelet additive solutions quality parameters single-donor platelets |
| title | First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution |
| title_full | First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution |
| title_fullStr | First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution |
| title_full_unstemmed | First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution |
| title_short | First Indian initiative for preparation of low-titer group “O” single-donor platelets with platelet additive solution |
| title_sort | first indian initiative for preparation of low titer group o single donor platelets with platelet additive solution |
| topic | Group O platelets low titer platelet additive solutions quality parameters single-donor platelets |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2018;volume=12;issue=1;spage=10;epage=16;aulast=Jain |
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