Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs
This study is aimed at the effects of low-protein diets with four amino acids balanced on serum biochemical parameters and colonic microflora of finishing pigs. Fifty-four healthy (Duroc × Landrace × Yorkshire) hybrid barrows with an average body weight of 70.12 ± 4.03 kg were randomly assigned to o...
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/9 |
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| author | Shanghang Liu Zhiyong Fan |
| author_facet | Shanghang Liu Zhiyong Fan |
| author_sort | Shanghang Liu |
| collection | DOAJ |
| description | This study is aimed at the effects of low-protein diets with four amino acids balanced on serum biochemical parameters and colonic microflora of finishing pigs. Fifty-four healthy (Duroc × Landrace × Yorkshire) hybrid barrows with an average body weight of 70.12 ± 4.03 kg were randomly assigned to one of three dietary treatments with three barrows per pen and six pens per treatment. The barrows were fed a normal protein diet (NP), a low-protein diet (LP), and a very low-protein diet (VLP). Compared with the NP diet, reduced dietary protein did not influence serum biochemical parameters (<i>p</i> > 0.05). The valeric acid was significantly increased with the VLP diet (<i>p</i> < 0.05). Compared with the NP diets, the abundance of Terrisporobacter (13.37%) Clostridium_sensu_stricto_1 (23.37%) and Turicibacter (2.57%) increased to 21.04, 33.42 and 13.68% in LP diets and 16.72, 43.71 and 14.61% in VLP diets, while the abundance of Lactobacillus (9.30%) and Streptococcus (25.26%) decreased to 3.57 and 14.50% in LP diets and 1.86 and 4.07% in VLP diets. Turicibacter and Clostridium_sensu_stricto_6 had a powerful negative correlation with the content of valeric acid (<i>p</i> < 0.01), while Peptococcus and Clostridia_UCG-014 had a very solid positive correlation (<i>p</i> < 0.01). In conclusion, reducing dietary protein level can improve colon microbiota composition, especially reducing the abundance of bacteria related to nitrogen metabolism, but has no significant effect on SCFA except valeric acid. In addition, reduction in the dietary protein level by 5.48% had more different flora than that of 2.74% reduction in dietary CP level. |
| first_indexed | 2024-03-11T10:12:41Z |
| format | Article |
| id | doaj.art-4df139faa6724a1b897244c97ef86930 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:12:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4df139faa6724a1b897244c97ef869302023-11-16T14:17:56ZengMDPI AGAnimals2076-26152022-12-01131910.3390/ani13010009Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing PigsShanghang Liu0Zhiyong Fan1Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaThis study is aimed at the effects of low-protein diets with four amino acids balanced on serum biochemical parameters and colonic microflora of finishing pigs. Fifty-four healthy (Duroc × Landrace × Yorkshire) hybrid barrows with an average body weight of 70.12 ± 4.03 kg were randomly assigned to one of three dietary treatments with three barrows per pen and six pens per treatment. The barrows were fed a normal protein diet (NP), a low-protein diet (LP), and a very low-protein diet (VLP). Compared with the NP diet, reduced dietary protein did not influence serum biochemical parameters (<i>p</i> > 0.05). The valeric acid was significantly increased with the VLP diet (<i>p</i> < 0.05). Compared with the NP diets, the abundance of Terrisporobacter (13.37%) Clostridium_sensu_stricto_1 (23.37%) and Turicibacter (2.57%) increased to 21.04, 33.42 and 13.68% in LP diets and 16.72, 43.71 and 14.61% in VLP diets, while the abundance of Lactobacillus (9.30%) and Streptococcus (25.26%) decreased to 3.57 and 14.50% in LP diets and 1.86 and 4.07% in VLP diets. Turicibacter and Clostridium_sensu_stricto_6 had a powerful negative correlation with the content of valeric acid (<i>p</i> < 0.01), while Peptococcus and Clostridia_UCG-014 had a very solid positive correlation (<i>p</i> < 0.01). In conclusion, reducing dietary protein level can improve colon microbiota composition, especially reducing the abundance of bacteria related to nitrogen metabolism, but has no significant effect on SCFA except valeric acid. In addition, reduction in the dietary protein level by 5.48% had more different flora than that of 2.74% reduction in dietary CP level.https://www.mdpi.com/2076-2615/13/1/9finishing pigsgut microbiotalow-protein dietscolonic microflora |
| spellingShingle | Shanghang Liu Zhiyong Fan Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs Animals finishing pigs gut microbiota low-protein diets colonic microflora |
| title | Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs |
| title_full | Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs |
| title_fullStr | Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs |
| title_full_unstemmed | Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs |
| title_short | Effects of Dietary Protein Restriction on Colonic Microbiota of Finishing Pigs |
| title_sort | effects of dietary protein restriction on colonic microbiota of finishing pigs |
| topic | finishing pigs gut microbiota low-protein diets colonic microflora |
| url | https://www.mdpi.com/2076-2615/13/1/9 |
| work_keys_str_mv | AT shanghangliu effectsofdietaryproteinrestrictiononcolonicmicrobiotaoffinishingpigs AT zhiyongfan effectsofdietaryproteinrestrictiononcolonicmicrobiotaoffinishingpigs |