Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus
A soluble agglutinin was purified by affinity chromatography of the celomic fluid of the marine worm Sipunculus nudus. This agglutinin requires metal cations for its activity and is specific for derivatives of D-galactose. It resulted lightly thermostable, with a pH optimum around 7.5. On SDSPAGE, i...
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2010-10-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/224 |
| _version_ | 1819236711807320064 |
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| author | L Ballarin M Del Favero |
| author_facet | L Ballarin M Del Favero |
| author_sort | L Ballarin |
| collection | DOAJ |
| description | A soluble agglutinin was purified by affinity chromatography of the celomic fluid of the marine worm Sipunculus nudus. This agglutinin requires metal cations for its activity and is specific for derivatives of D-galactose. It resulted lightly thermostable, with a pH optimum around 7.5. On SDSPAGE, it was resolved in two bands, of 33 and 31 kDa in reducing conditions and 29 and 26 kDa in non-reducing conditions. This behavior is probably due to the presence of disulfide bridges between cysteine residues, which are required for the correct functioning of the hemagglutinin, as β- mercaptoethanol completely abolish the agglutinating activity of cell-free celomic fluid. The purified lectin can influence in vitro phagocytosis of yeast by celomic leukocytes: in the presence of the molecule, ingestion of foreign particles results significantly decreased and yeast cells agglutinate and forms rosettes around the celomocytes. This suggests a role of the molecule in immunosurveillance. |
| first_indexed | 2024-12-23T13:08:47Z |
| format | Article |
| id | doaj.art-4df142df9314425d8b678472a0cad090 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-23T13:08:47Z |
| publishDate | 2010-10-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-4df142df9314425d8b678472a0cad0902022-12-21T17:45:48ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2010-10-0172Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudusL Ballarin0M Del Favero1Department of Biology, University of Padua, Padua, ItalyDepartment of Biology, University of Padua, Padua, ItalyA soluble agglutinin was purified by affinity chromatography of the celomic fluid of the marine worm Sipunculus nudus. This agglutinin requires metal cations for its activity and is specific for derivatives of D-galactose. It resulted lightly thermostable, with a pH optimum around 7.5. On SDSPAGE, it was resolved in two bands, of 33 and 31 kDa in reducing conditions and 29 and 26 kDa in non-reducing conditions. This behavior is probably due to the presence of disulfide bridges between cysteine residues, which are required for the correct functioning of the hemagglutinin, as β- mercaptoethanol completely abolish the agglutinating activity of cell-free celomic fluid. The purified lectin can influence in vitro phagocytosis of yeast by celomic leukocytes: in the presence of the molecule, ingestion of foreign particles results significantly decreased and yeast cells agglutinate and forms rosettes around the celomocytes. This suggests a role of the molecule in immunosurveillance.https://isj02.unimore.it/index.php/ISJ/article/view/224Sipunculusinvertebratescelomic fluidhemagglutininCa2 -dependent lectin |
| spellingShingle | L Ballarin M Del Favero Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus Invertebrate Survival Journal Sipunculus invertebrates celomic fluid hemagglutinin Ca2 -dependent lectin |
| title | Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus |
| title_full | Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus |
| title_fullStr | Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus |
| title_full_unstemmed | Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus |
| title_short | Identification and preliminary characterization of a Ca2+-dependent hemagglutinin in the celomic fluid of Sipunculus nudus |
| title_sort | identification and preliminary characterization of a ca2 dependent hemagglutinin in the celomic fluid of sipunculus nudus |
| topic | Sipunculus invertebrates celomic fluid hemagglutinin Ca2 -dependent lectin |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/224 |
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