Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods
The emergence of antibiotic-resistant Escherichia coli, especially extended–spectrum β-lactamase (ESBL)–producing strains, in the intestinal tract of broilers could be a threat to poultry and human. We investigated changes of antimicrobial resistance patterns and frequency of ESBL genes among faecal...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-07-01
|
| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2017.1415703 |
| _version_ | 1823926009139822592 |
|---|---|
| author | Fatemeh Doregiraee Masoud Alebouyeh Bahar Nayeri Fasaei Saeed Charkhkar Elahe Tajeddin Mohammad Reza Zali |
| author_facet | Fatemeh Doregiraee Masoud Alebouyeh Bahar Nayeri Fasaei Saeed Charkhkar Elahe Tajeddin Mohammad Reza Zali |
| author_sort | Fatemeh Doregiraee |
| collection | DOAJ |
| description | The emergence of antibiotic-resistant Escherichia coli, especially extended–spectrum β-lactamase (ESBL)–producing strains, in the intestinal tract of broilers could be a threat to poultry and human. We investigated changes of antimicrobial resistance patterns and frequency of ESBL genes among faecal E. coli isolates of broilers and workers in five different farms during two rearing periods in Iran. In this regard, E. coli was isolated from rectal swabs of the workers and cloacal swabs of the broilers. After detection of antibiotic resistance patterns, phenotypic and genotypic characterisation of ESBL phenotype in these strains, carriage of the resistance genes on their crude plasmid extracts and diversity of plasmid profiles were analysed. Accordingly, multidrug-resistant (MDR) patterns were detected in a high percentage of E. coli strains from the workers (72.7%) and poultry (92.3%). ESBL-producing E. coli strains were identified in these farms throughout the two periods of rearing (6.3%). blaCTX-M-1, blaCTX-M-61, blaTEM-116 and blaTEM-1 were characterised in 6 (Period I/II: 4/2), 1 (Period I), 2 (Period II) and 9 (Period I/II: 5/4) strains, respectively. The first isolation of E. coli strains harbouring the blaTEM116 gene in chicken is reported in this study. In conclusion, results of this study showed that chickens could serve as a reservoir for ESBL-producing E. coli strains. These strains could carry clinically important ESBL or new emerging β-lactamases genes. Early colonisation and selection of the resistant strains during rearing periods proposed illegal use of antimicrobials as the cause of change in resistance patterns in the studied farms. |
| first_indexed | 2024-12-16T20:17:44Z |
| format | Article |
| id | doaj.art-4df1b6ae957d41bb9a631e018efe76b3 |
| institution | Directory Open Access Journal |
| issn | 1828-051X |
| language | English |
| last_indexed | 2024-12-16T20:17:44Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-4df1b6ae957d41bb9a631e018efe76b32022-12-21T22:17:53ZengTaylor & Francis GroupItalian Journal of Animal Science1828-051X2018-07-0117381582410.1080/1828051X.2017.14157031415703Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periodsFatemeh Doregiraee0Masoud Alebouyeh1Bahar Nayeri Fasaei2Saeed Charkhkar3Elahe Tajeddin4Mohammad Reza Zali5University of TehranShahid Beheshti University of Medical SciencesUniversity of TehranIslamic Azad University, Science and Research BranchShahid Beheshti University of Medical SciencesShahid Beheshti University of Medical SciencesThe emergence of antibiotic-resistant Escherichia coli, especially extended–spectrum β-lactamase (ESBL)–producing strains, in the intestinal tract of broilers could be a threat to poultry and human. We investigated changes of antimicrobial resistance patterns and frequency of ESBL genes among faecal E. coli isolates of broilers and workers in five different farms during two rearing periods in Iran. In this regard, E. coli was isolated from rectal swabs of the workers and cloacal swabs of the broilers. After detection of antibiotic resistance patterns, phenotypic and genotypic characterisation of ESBL phenotype in these strains, carriage of the resistance genes on their crude plasmid extracts and diversity of plasmid profiles were analysed. Accordingly, multidrug-resistant (MDR) patterns were detected in a high percentage of E. coli strains from the workers (72.7%) and poultry (92.3%). ESBL-producing E. coli strains were identified in these farms throughout the two periods of rearing (6.3%). blaCTX-M-1, blaCTX-M-61, blaTEM-116 and blaTEM-1 were characterised in 6 (Period I/II: 4/2), 1 (Period I), 2 (Period II) and 9 (Period I/II: 5/4) strains, respectively. The first isolation of E. coli strains harbouring the blaTEM116 gene in chicken is reported in this study. In conclusion, results of this study showed that chickens could serve as a reservoir for ESBL-producing E. coli strains. These strains could carry clinically important ESBL or new emerging β-lactamases genes. Early colonisation and selection of the resistant strains during rearing periods proposed illegal use of antimicrobials as the cause of change in resistance patterns in the studied farms.http://dx.doi.org/10.1080/1828051X.2017.1415703ESBLEscherichia coliMDRpoultryhuman |
| spellingShingle | Fatemeh Doregiraee Masoud Alebouyeh Bahar Nayeri Fasaei Saeed Charkhkar Elahe Tajeddin Mohammad Reza Zali Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods Italian Journal of Animal Science ESBL Escherichia coli MDR poultry human |
| title | Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods |
| title_full | Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods |
| title_fullStr | Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods |
| title_full_unstemmed | Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods |
| title_short | Changes in antimicrobial resistance patterns and dominance of extended spectrum β-lactamase genes among faecal Escherichia coli isolates from broilers and workers during two rearing periods |
| title_sort | changes in antimicrobial resistance patterns and dominance of extended spectrum β lactamase genes among faecal escherichia coli isolates from broilers and workers during two rearing periods |
| topic | ESBL Escherichia coli MDR poultry human |
| url | http://dx.doi.org/10.1080/1828051X.2017.1415703 |
| work_keys_str_mv | AT fatemehdoregiraee changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods AT masoudalebouyeh changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods AT baharnayerifasaei changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods AT saeedcharkhkar changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods AT elahetajeddin changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods AT mohammadrezazali changesinantimicrobialresistancepatternsanddominanceofextendedspectrumblactamasegenesamongfaecalescherichiacoliisolatesfrombroilersandworkersduringtworearingperiods |