Surface Analysis of Native Spider Draglines by FE-SEM and XPS
Although the physical and biological functions of the skin layer of spider dragline have been studied and partially clarified, the morphology and elemental contents of the skin layer of silk fibers have not been investigated in detail to date. Here, the surface of Nephila clavata spider dragline was...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00231/full |
| _version_ | 1811288734126047232 |
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| author | Hiromitsu Sogawa Kyohei Nakano Ayaka Tateishi Keisuke Tajima Keiji Numata |
| author_facet | Hiromitsu Sogawa Kyohei Nakano Ayaka Tateishi Keisuke Tajima Keiji Numata |
| author_sort | Hiromitsu Sogawa |
| collection | DOAJ |
| description | Although the physical and biological functions of the skin layer of spider dragline have been studied and partially clarified, the morphology and elemental contents of the skin layer of silk fibers have not been investigated in detail to date. Here, the surface of Nephila clavata spider dragline was evaluated by field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) to obtain clear surface morphological and molecular information. The FE-SEM images of the spider dragline indicate that the spider dragline forms a bundle of microfibrils. This hierarchical structure might induce faint fibrilar and network-like patterns on the surface of the dragline. XPS analysis revealed the presence of Na, P, and S, which are reasonably explained by considering the biological components of the major ampullate gland of spiders. The results obtained here are preliminary but will be important to consider the molecular transition of silk proteins to form excellent hierarchical structures during the spider dragline spinning process. |
| first_indexed | 2024-04-13T03:42:24Z |
| format | Article |
| id | doaj.art-4df241c43d7d49f3ab7869ca0923e491 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-13T03:42:24Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4df241c43d7d49f3ab7869ca0923e4912022-12-22T03:04:07ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-03-01810.3389/fbioe.2020.00231498095Surface Analysis of Native Spider Draglines by FE-SEM and XPSHiromitsu Sogawa0Kyohei Nakano1Ayaka Tateishi2Keisuke Tajima3Keiji Numata4Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama, JapanEmergent Functional Polymers Research Team, RIKEN Center for Emergent Matter Science, Saitama, JapanBiomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama, JapanEmergent Functional Polymers Research Team, RIKEN Center for Emergent Matter Science, Saitama, JapanBiomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Saitama, JapanAlthough the physical and biological functions of the skin layer of spider dragline have been studied and partially clarified, the morphology and elemental contents of the skin layer of silk fibers have not been investigated in detail to date. Here, the surface of Nephila clavata spider dragline was evaluated by field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) to obtain clear surface morphological and molecular information. The FE-SEM images of the spider dragline indicate that the spider dragline forms a bundle of microfibrils. This hierarchical structure might induce faint fibrilar and network-like patterns on the surface of the dragline. XPS analysis revealed the presence of Na, P, and S, which are reasonably explained by considering the biological components of the major ampullate gland of spiders. The results obtained here are preliminary but will be important to consider the molecular transition of silk proteins to form excellent hierarchical structures during the spider dragline spinning process.https://www.frontiersin.org/article/10.3389/fbioe.2020.00231/fullspider draglinesilkwormsilk fiberFE-SEMXPSsurface analysis |
| spellingShingle | Hiromitsu Sogawa Kyohei Nakano Ayaka Tateishi Keisuke Tajima Keiji Numata Surface Analysis of Native Spider Draglines by FE-SEM and XPS Frontiers in Bioengineering and Biotechnology spider dragline silkworm silk fiber FE-SEM XPS surface analysis |
| title | Surface Analysis of Native Spider Draglines by FE-SEM and XPS |
| title_full | Surface Analysis of Native Spider Draglines by FE-SEM and XPS |
| title_fullStr | Surface Analysis of Native Spider Draglines by FE-SEM and XPS |
| title_full_unstemmed | Surface Analysis of Native Spider Draglines by FE-SEM and XPS |
| title_short | Surface Analysis of Native Spider Draglines by FE-SEM and XPS |
| title_sort | surface analysis of native spider draglines by fe sem and xps |
| topic | spider dragline silkworm silk fiber FE-SEM XPS surface analysis |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00231/full |
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