Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride
This study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride and to investigate the skin penetration pathways of these microemulsions by colocalization techniques using confocal laser scanning microscopy (CLSM). The prepared finasterid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2784 |
| _version_ | 1797455712602816512 |
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| author | Thirapit Subongkot Natthan Charernsriwilaiwat Rattathammanoon Chanasongkram Kantawat Rittem Tanasait Ngawhirunpat Praneet Opanasopit |
| author_facet | Thirapit Subongkot Natthan Charernsriwilaiwat Rattathammanoon Chanasongkram Kantawat Rittem Tanasait Ngawhirunpat Praneet Opanasopit |
| author_sort | Thirapit Subongkot |
| collection | DOAJ |
| description | This study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride and to investigate the skin penetration pathways of these microemulsions by colocalization techniques using confocal laser scanning microscopy (CLSM). The prepared finasteride-loaded microemulsions had average particle sizes ranging from 80.09 to 136.97 nm with particle size distributions within acceptable ranges and exhibited negative surface charges. The obtained microemulsions could significantly increase the skin penetration of finasteride compared to a finasteride solution. According to the skin penetration pathway evaluation conducted with CLSM, the microemulsions were hair follicle-targeted formulations due to penetration via the transfollicular pathway as a major skin penetration pathway. Additionally, this study found that the microemulsions also penetrated via the intercluster pathway more than via the intercellular pathway and transcellular pathway. The intercluster pathway, intercellular pathway, and transcellular pathway were considered only minor pathways. |
| first_indexed | 2024-03-09T15:58:16Z |
| format | Article |
| id | doaj.art-4df3da13f6ac45b7bee9e1208500e773 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:16Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-4df3da13f6ac45b7bee9e1208500e7732023-11-24T17:21:58ZengMDPI AGPharmaceutics1999-49232022-12-011412278410.3390/pharmaceutics14122784Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of FinasterideThirapit Subongkot0Natthan Charernsriwilaiwat1Rattathammanoon Chanasongkram2Kantawat Rittem3Tanasait Ngawhirunpat4Praneet Opanasopit5Research Unit of Pharmaceutical Innovations of Natural Products Unit (PhInNat), Faculty of Pharmaceutical Sciences, Burapha University, Saen Suk, Mueang, Chonburi 20131, ThailandResearch Unit of Pharmaceutical Innovations of Natural Products Unit (PhInNat), Faculty of Pharmaceutical Sciences, Burapha University, Saen Suk, Mueang, Chonburi 20131, ThailandYainthai Company Limited, Nonthaburi 11150, ThailandDepartment of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Burapha University, Saen Suk, Mueang, Chonburi 20131, ThailandDepartment of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandThis study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride and to investigate the skin penetration pathways of these microemulsions by colocalization techniques using confocal laser scanning microscopy (CLSM). The prepared finasteride-loaded microemulsions had average particle sizes ranging from 80.09 to 136.97 nm with particle size distributions within acceptable ranges and exhibited negative surface charges. The obtained microemulsions could significantly increase the skin penetration of finasteride compared to a finasteride solution. According to the skin penetration pathway evaluation conducted with CLSM, the microemulsions were hair follicle-targeted formulations due to penetration via the transfollicular pathway as a major skin penetration pathway. Additionally, this study found that the microemulsions also penetrated via the intercluster pathway more than via the intercellular pathway and transcellular pathway. The intercluster pathway, intercellular pathway, and transcellular pathway were considered only minor pathways.https://www.mdpi.com/1999-4923/14/12/2784microemulsionsfinasteridepoloxamer 124skin penetration pathwayconfocal laser scanning microscopy |
| spellingShingle | Thirapit Subongkot Natthan Charernsriwilaiwat Rattathammanoon Chanasongkram Kantawat Rittem Tanasait Ngawhirunpat Praneet Opanasopit Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride Pharmaceutics microemulsions finasteride poloxamer 124 skin penetration pathway confocal laser scanning microscopy |
| title | Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride |
| title_full | Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride |
| title_fullStr | Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride |
| title_full_unstemmed | Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride |
| title_short | Development and Skin Penetration Pathway Evaluation Using Confocal Laser Scanning Microscopy of Microemulsions for Dermal Delivery Enhancement of Finasteride |
| title_sort | development and skin penetration pathway evaluation using confocal laser scanning microscopy of microemulsions for dermal delivery enhancement of finasteride |
| topic | microemulsions finasteride poloxamer 124 skin penetration pathway confocal laser scanning microscopy |
| url | https://www.mdpi.com/1999-4923/14/12/2784 |
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