Evaluation of transplantation sites for human intestinal organoids.
Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison betwe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0237885 |
| _version_ | 1797810913707819008 |
|---|---|
| author | Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath |
| author_facet | Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath |
| author_sort | Akaljot Singh |
| collection | DOAJ |
| description | Our group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison between models has not yet been performed. Embryonic stem cells were differentiated into HIOs, as previously described. HIOs from the same batch were transplanted on the same day into either the RSS or mesentery. 10 weeks were allowed for engraftment and differentiation, at which time they were harvested and assessed. Metrics for comparison included: mortality, engraftment rate, gross size, number and grade of lumens, and expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism. Mortality was significantly increased when undergoing mesentery transplantation, however engraftment was significantly higher. Graft sizes were similar between groups. Morphometric parameters were similar between groups, however m-tHIOs presented with significantly fewer lumens than k-tHIO. Transcript and protein level expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism were similar between groups. Transplantation into both sites yields viable tissue of similar quality based on our assessments with enhanced engraftment and a dominant lumen for uniform study benefiting the mesenteric site and survival benefiting RSS. |
| first_indexed | 2024-03-13T07:15:53Z |
| format | Article |
| id | doaj.art-4df5319f91e4406ea55d08281fbc7442 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T07:15:53Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4df5319f91e4406ea55d08281fbc74422023-06-05T05:32:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023788510.1371/journal.pone.0237885Evaluation of transplantation sites for human intestinal organoids.Akaljot SinghHolly M PolingNambirajan SundaramNicole BrownJames M WellsMichael A HelmrathOur group has developed two transplantation models for the engraftment of Human Intestinal Organoids (HIOs): the renal subcapsular space (RSS) and the mesentery each with specific benefits for study. While engraftment at both sites generates laminated intestinal structures, a direct comparison between models has not yet been performed. Embryonic stem cells were differentiated into HIOs, as previously described. HIOs from the same batch were transplanted on the same day into either the RSS or mesentery. 10 weeks were allowed for engraftment and differentiation, at which time they were harvested and assessed. Metrics for comparison included: mortality, engraftment rate, gross size, number and grade of lumens, and expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism. Mortality was significantly increased when undergoing mesentery transplantation, however engraftment was significantly higher. Graft sizes were similar between groups. Morphometric parameters were similar between groups, however m-tHIOs presented with significantly fewer lumens than k-tHIO. Transcript and protein level expression of markers specific to epithelial differentiation, mesenchymal differentiation, and carbohydrate metabolism were similar between groups. Transplantation into both sites yields viable tissue of similar quality based on our assessments with enhanced engraftment and a dominant lumen for uniform study benefiting the mesenteric site and survival benefiting RSS.https://doi.org/10.1371/journal.pone.0237885 |
| spellingShingle | Akaljot Singh Holly M Poling Nambirajan Sundaram Nicole Brown James M Wells Michael A Helmrath Evaluation of transplantation sites for human intestinal organoids. PLoS ONE |
| title | Evaluation of transplantation sites for human intestinal organoids. |
| title_full | Evaluation of transplantation sites for human intestinal organoids. |
| title_fullStr | Evaluation of transplantation sites for human intestinal organoids. |
| title_full_unstemmed | Evaluation of transplantation sites for human intestinal organoids. |
| title_short | Evaluation of transplantation sites for human intestinal organoids. |
| title_sort | evaluation of transplantation sites for human intestinal organoids |
| url | https://doi.org/10.1371/journal.pone.0237885 |
| work_keys_str_mv | AT akaljotsingh evaluationoftransplantationsitesforhumanintestinalorganoids AT hollympoling evaluationoftransplantationsitesforhumanintestinalorganoids AT nambirajansundaram evaluationoftransplantationsitesforhumanintestinalorganoids AT nicolebrown evaluationoftransplantationsitesforhumanintestinalorganoids AT jamesmwells evaluationoftransplantationsitesforhumanintestinalorganoids AT michaelahelmrath evaluationoftransplantationsitesforhumanintestinalorganoids |