Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India
Aim: The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). Materials and Methods: A total of 2444 sera samples (675 sheep and 1769 goat) from unorg...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2018-08-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.11/August-2018/2.pdf |
| _version_ | 1818591804067414016 |
|---|---|
| author | A. Kanani S. Dabhi Y. Patel V. Chandra O. R. Vinodh Kumar R. Shome |
| author_facet | A. Kanani S. Dabhi Y. Patel V. Chandra O. R. Vinodh Kumar R. Shome |
| author_sort | A. Kanani |
| collection | DOAJ |
| description | Aim: The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA).
Materials and Methods: A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening.
Results: In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01).
Conclusion: The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures. |
| first_indexed | 2024-12-16T10:18:15Z |
| format | Article |
| id | doaj.art-4dfb5d30edb24a349c92281398fe9d4f |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T10:18:15Z |
| publishDate | 2018-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-4dfb5d30edb24a349c92281398fe9d4f2022-12-21T22:35:23ZengVeterinary WorldVeterinary World0972-89882231-09162018-08-011181030103610.14202/vetworld.2018.1030-1036Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, IndiaA. Kanani0S. Dabhi1Y. Patel2V. Chandra3O. R. Vinodh Kumar4R. Shome5Office of Deputy Director of Animal Husbandry, F.M.D. Typing Scheme, Polytechnic Campus, Ambawadi, Ahmedabad - 380 015, Gujarat, India.Office of Deputy Director of Animal Husbandry, F.M.D. Typing Scheme, Polytechnic Campus, Ambawadi, Ahmedabad - 380 015, Gujarat, India.Department of Biomedical Technology, University School of Science, Gujarat University, Ahmedabad, Gujarat.Office of Deputy Director of Animal Husbandry, F.M.D. Typing Scheme, Polytechnic Campus, Ambawadi, Ahmedabad - 380 015, Gujarat, India.ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Yelahanka, Bengaluru - 560 064, Karnataka, India.Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.Aim: The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). Materials and Methods: A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening. Results: In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01). Conclusion: The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures.http://www.veterinaryworld.org/Vol.11/August-2018/2.pdfbrucellosisGujaratindirect enzyme-linked immunosorbent assayRose Bengal Plate testseroprevalencesmall ruminants |
| spellingShingle | A. Kanani S. Dabhi Y. Patel V. Chandra O. R. Vinodh Kumar R. Shome Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India Veterinary World brucellosis Gujarat indirect enzyme-linked immunosorbent assay Rose Bengal Plate test seroprevalence small ruminants |
| title | Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India |
| title_full | Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India |
| title_fullStr | Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India |
| title_full_unstemmed | Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India |
| title_short | Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India |
| title_sort | seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of gujarat state india |
| topic | brucellosis Gujarat indirect enzyme-linked immunosorbent assay Rose Bengal Plate test seroprevalence small ruminants |
| url | http://www.veterinaryworld.org/Vol.11/August-2018/2.pdf |
| work_keys_str_mv | AT akanani seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia AT sdabhi seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia AT ypatel seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia AT vchandra seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia AT orvinodhkumar seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia AT rshome seroprevalenceofbrucellosisinsmallruminantsinorganizedandunorganizedsectorsofgujaratstateindia |