<i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato
Most flowering plant species contain at least two copies of the <i>DEFECTIVE EMBRYO AND MERISTEMS</i> (<i>DEM</i>) gene with the encoded DEM proteins lacking homology to proteins of known biochemical function. In tomato (<i>Sl</i>; <i>Solanum lycopersicum<...
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/19/2545 |
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| author | Louisa Matthew Melquiades E. C. Reyes Christopher W. G. Mann Alasdair W. McDowall Andrew L. Eamens Bernard J. Carroll |
| author_facet | Louisa Matthew Melquiades E. C. Reyes Christopher W. G. Mann Alasdair W. McDowall Andrew L. Eamens Bernard J. Carroll |
| author_sort | Louisa Matthew |
| collection | DOAJ |
| description | Most flowering plant species contain at least two copies of the <i>DEFECTIVE EMBRYO AND MERISTEMS</i> (<i>DEM</i>) gene with the encoded DEM proteins lacking homology to proteins of known biochemical function. In tomato (<i>Sl</i>; <i>Solanum lycopersicum</i>), stable mutations in the <i>SlDEM1</i> locus result in shoot and root meristem defects with the <i>dem1</i> mutant failing to progress past the cotyledon stage of seedling development. Generation of a <i>Somatic Mutagenesis of DEM1</i> (<i>SMD</i>) transformant line in tomato allowed for the characterization of <i>SlDEM1</i> gene function past the seedling stage of vegetative development with <i>SMD</i> plants displaying a range of leaf development abnormalities. Further, the sectored or stable <i>in planta</i> expression of specific regions of the <i>SlDEM1</i> coding sequence also resulted in the generation of tomato transformants that displayed a range of vegetative development defects, which when considered together with the <i>dem1</i> mutant seedling and <i>SMD</i> transformant line phenotypic data, allowed for the assignment of <i>SlDEM1</i> gene function to early embryo development, adaxial epidermis cell development, lateral leaf blade expansion, and mesophyll cell proliferation and differentiation. |
| first_indexed | 2024-03-09T21:17:46Z |
| format | Article |
| id | doaj.art-4e036ebffb73402c8f03ba3e61c9bf21 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:17:46Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4e036ebffb73402c8f03ba3e61c9bf212023-11-23T21:29:14ZengMDPI AGPlants2223-77472022-09-011119254510.3390/plants11192545<i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in TomatoLouisa Matthew0Melquiades E. C. Reyes1Christopher W. G. Mann2Alasdair W. McDowall3Andrew L. Eamens4Bernard J. Carroll5School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, AustraliaCentre for Microscopy and Microanalysis, The University of Queensland, St. Lucia, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, AustraliaSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, AustraliaMost flowering plant species contain at least two copies of the <i>DEFECTIVE EMBRYO AND MERISTEMS</i> (<i>DEM</i>) gene with the encoded DEM proteins lacking homology to proteins of known biochemical function. In tomato (<i>Sl</i>; <i>Solanum lycopersicum</i>), stable mutations in the <i>SlDEM1</i> locus result in shoot and root meristem defects with the <i>dem1</i> mutant failing to progress past the cotyledon stage of seedling development. Generation of a <i>Somatic Mutagenesis of DEM1</i> (<i>SMD</i>) transformant line in tomato allowed for the characterization of <i>SlDEM1</i> gene function past the seedling stage of vegetative development with <i>SMD</i> plants displaying a range of leaf development abnormalities. Further, the sectored or stable <i>in planta</i> expression of specific regions of the <i>SlDEM1</i> coding sequence also resulted in the generation of tomato transformants that displayed a range of vegetative development defects, which when considered together with the <i>dem1</i> mutant seedling and <i>SMD</i> transformant line phenotypic data, allowed for the assignment of <i>SlDEM1</i> gene function to early embryo development, adaxial epidermis cell development, lateral leaf blade expansion, and mesophyll cell proliferation and differentiation.https://www.mdpi.com/2223-7747/11/19/2545tomato (<i>Solanum lycopersicum</i>)<i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) genecell proliferationcell differentiationvegetative developmentleaf development |
| spellingShingle | Louisa Matthew Melquiades E. C. Reyes Christopher W. G. Mann Alasdair W. McDowall Andrew L. Eamens Bernard J. Carroll <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato Plants tomato (<i>Solanum lycopersicum</i>) <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) gene cell proliferation cell differentiation vegetative development leaf development |
| title | <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato |
| title_full | <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato |
| title_fullStr | <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato |
| title_full_unstemmed | <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato |
| title_short | <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) Is Essential for Cell Proliferation and Cell Differentiation in Tomato |
| title_sort | i defective embryo and meristems1 i i dem1 i is essential for cell proliferation and cell differentiation in tomato |
| topic | tomato (<i>Solanum lycopersicum</i>) <i>DEFECTIVE EMBRYO AND MERISTEMS1</i> (<i>DEM1</i>) gene cell proliferation cell differentiation vegetative development leaf development |
| url | https://www.mdpi.com/2223-7747/11/19/2545 |
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