Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia
Biofertilizer as an amendment has growing awareness. Little attention has been paid to bioremediation potential of indigenous heavy-metal-resistant microbes, especially when isolated from long-term polluted soil, as a bioinoculant in biofertilizers. Biofertilizers are a type of versatile nutrient pr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/20/3651 |
| _version_ | 1797572456488108032 |
|---|---|
| author | Ladda Seang-On Weeradej Meeinkuirt Preeyaporn Koedrith |
| author_facet | Ladda Seang-On Weeradej Meeinkuirt Preeyaporn Koedrith |
| author_sort | Ladda Seang-On |
| collection | DOAJ |
| description | Biofertilizer as an amendment has growing awareness. Little attention has been paid to bioremediation potential of indigenous heavy-metal-resistant microbes, especially when isolated from long-term polluted soil, as a bioinoculant in biofertilizers. Biofertilizers are a type of versatile nutrient provider and soil conditioner that is cost-competitive and highly efficient with nondisruptive detoxifying capability. Herein, we investigated the effect of biofertilizers containing indigenous cadmium (Cd)-resistant microbial consortia on rice growth and physiological response. The Thai rice cultivar PSL2 (<i>Oryza sativa</i> L.) was grown in Cd-enriched soils amended with 3% biofertilizer. The composition of the biofertilizers’ bacterial community at different taxonomic levels was explored using 16S rRNA gene Illumina MiSeq sequencing. Upon Cd stress, the test biofertilizer had maximum mitigating effects as shown by modulating photosynthetic pigment, MDA and proline content and enzymatic antioxidants, thereby allowing increased shoot and root biomass (46% and 53%, respectively) and reduced grain Cd content, as compared to the control. These phenomena might be attributed to increased soil pH and organic matter, as well as enriched beneficial detoxifiers, i.e., <i>Bacteroidetes, Firmicutes</i> and <i>Proteobacteria</i>, in the biofertilizers. The test biofertilizer was effective in alleviating Cd stress by improving soil biophysicochemical traits to limit Cd bioavailability, along with adjusting physiological traits such as antioxidative defense. This study first demonstrated that incorporating biofertilizer derived from indigenous Cd-resistant microbes could restrict Cd contents and consequently enhance plant growth and tolerance in polluted soil. |
| first_indexed | 2024-03-10T20:56:33Z |
| format | Article |
| id | doaj.art-4e06a93227064e0b9f8083f05e8f457c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:56:33Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4e06a93227064e0b9f8083f05e8f457c2023-11-19T17:50:02ZengMDPI AGPlants2223-77472023-10-011220365110.3390/plants12203651Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial ConsortiaLadda Seang-On0Weeradej Meeinkuirt1Preeyaporn Koedrith2Faculty of Environment and Resource Studies, Mahidol University, 999 Phuttamonthon District, Nakhon Pathom 73170, ThailandWater and Soil Environmental Research Unit, Nakhonsawan Campus, Mahidol University, Nakhonsawan 60130, ThailandFaculty of Environment and Resource Studies, Mahidol University, 999 Phuttamonthon District, Nakhon Pathom 73170, ThailandBiofertilizer as an amendment has growing awareness. Little attention has been paid to bioremediation potential of indigenous heavy-metal-resistant microbes, especially when isolated from long-term polluted soil, as a bioinoculant in biofertilizers. Biofertilizers are a type of versatile nutrient provider and soil conditioner that is cost-competitive and highly efficient with nondisruptive detoxifying capability. Herein, we investigated the effect of biofertilizers containing indigenous cadmium (Cd)-resistant microbial consortia on rice growth and physiological response. The Thai rice cultivar PSL2 (<i>Oryza sativa</i> L.) was grown in Cd-enriched soils amended with 3% biofertilizer. The composition of the biofertilizers’ bacterial community at different taxonomic levels was explored using 16S rRNA gene Illumina MiSeq sequencing. Upon Cd stress, the test biofertilizer had maximum mitigating effects as shown by modulating photosynthetic pigment, MDA and proline content and enzymatic antioxidants, thereby allowing increased shoot and root biomass (46% and 53%, respectively) and reduced grain Cd content, as compared to the control. These phenomena might be attributed to increased soil pH and organic matter, as well as enriched beneficial detoxifiers, i.e., <i>Bacteroidetes, Firmicutes</i> and <i>Proteobacteria</i>, in the biofertilizers. The test biofertilizer was effective in alleviating Cd stress by improving soil biophysicochemical traits to limit Cd bioavailability, along with adjusting physiological traits such as antioxidative defense. This study first demonstrated that incorporating biofertilizer derived from indigenous Cd-resistant microbes could restrict Cd contents and consequently enhance plant growth and tolerance in polluted soil.https://www.mdpi.com/2223-7747/12/20/3651bioremediationbiofertilizerCd-resistant microbial consortiaphytotoxicityindigenous soil microorganisms |
| spellingShingle | Ladda Seang-On Weeradej Meeinkuirt Preeyaporn Koedrith Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia Plants bioremediation biofertilizer Cd-resistant microbial consortia phytotoxicity indigenous soil microorganisms |
| title | Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia |
| title_full | Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia |
| title_fullStr | Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia |
| title_full_unstemmed | Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia |
| title_short | Alleviation of Cadmium Toxicity in Thai Rice Cultivar (PSL2) Using Biofertilizer Containing Indigenous Cadmium-Resistant Microbial Consortia |
| title_sort | alleviation of cadmium toxicity in thai rice cultivar psl2 using biofertilizer containing indigenous cadmium resistant microbial consortia |
| topic | bioremediation biofertilizer Cd-resistant microbial consortia phytotoxicity indigenous soil microorganisms |
| url | https://www.mdpi.com/2223-7747/12/20/3651 |
| work_keys_str_mv | AT laddaseangon alleviationofcadmiumtoxicityinthairicecultivarpsl2usingbiofertilizercontainingindigenouscadmiumresistantmicrobialconsortia AT weeradejmeeinkuirt alleviationofcadmiumtoxicityinthairicecultivarpsl2usingbiofertilizercontainingindigenouscadmiumresistantmicrobialconsortia AT preeyapornkoedrith alleviationofcadmiumtoxicityinthairicecultivarpsl2usingbiofertilizercontainingindigenouscadmiumresistantmicrobialconsortia |