Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtain...
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MDPI AG
2022-04-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/12/4/648 |
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| author | Cristina Blanco-Elices Jesús Chato-Astrain Alberto González-González David Sánchez-Porras Víctor Carriel Ricardo Fernández-Valadés María del Carmen Sánchez-Quevedo Miguel Alaminos Ingrid Garzón |
| author_facet | Cristina Blanco-Elices Jesús Chato-Astrain Alberto González-González David Sánchez-Porras Víctor Carriel Ricardo Fernández-Valadés María del Carmen Sánchez-Quevedo Miguel Alaminos Ingrid Garzón |
| author_sort | Cristina Blanco-Elices |
| collection | DOAJ |
| description | The embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtained and histologically prepared for in the situ analysis of four hUC regions (intervascular—IV, perivascular—PV, subaminoblastic—SAM, and Wharton’s jelly—WH), and primary cell cultures of mesenchymal stem cells (hUC-MSC) isolated from each region were obtained. The results confirmed the heterogeneity of the hUC, with the IV and PV zones tending to show the higher in situ expression of several components of the extracellular matrix (collagens, proteoglycans, and glycosaminoglycans), vimentin, and MSC markers (especially CD73), although isolation and ex vivo culture resulted in a homogeneous cell profile. Three vascular markers were positive in situ, especially vWF, followed by CD34 and CD31, and isolation and culture revealed that the region associated with the highest expression of vascular markers was IV, followed by PV. These results confirm the heterogeneity of the hUC and the need for selecting cells from specific regions of the hUC for particular applications in tissue engineering. |
| first_indexed | 2024-03-09T13:26:11Z |
| format | Article |
| id | doaj.art-4e092bd65f2449478b48bc89b5c06b60 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-09T13:26:11Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-4e092bd65f2449478b48bc89b5c06b602023-11-30T21:23:18ZengMDPI AGJournal of Personalized Medicine2075-44262022-04-0112464810.3390/jpm12040648Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue EngineeringCristina Blanco-Elices0Jesús Chato-Astrain1Alberto González-González2David Sánchez-Porras3Víctor Carriel4Ricardo Fernández-Valadés5María del Carmen Sánchez-Quevedo6Miguel Alaminos7Ingrid Garzón8Doctoral Programme in Biomedicine, University of Granada, E18071 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainDepartment of Molecular Biology (IDIVAL), Faculty of Medicine, University of Cantabria, E39011 Santander, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, E18016 Granada, SpainThe embryonic development of the human umbilical cord (hUC) is complex, and different regions can be identified in this structure. The aim of this work is to characterize the hUC at in situ and ex vivo levels to stablish their potential use in vascular regeneration. Human umbilical cords were obtained and histologically prepared for in the situ analysis of four hUC regions (intervascular—IV, perivascular—PV, subaminoblastic—SAM, and Wharton’s jelly—WH), and primary cell cultures of mesenchymal stem cells (hUC-MSC) isolated from each region were obtained. The results confirmed the heterogeneity of the hUC, with the IV and PV zones tending to show the higher in situ expression of several components of the extracellular matrix (collagens, proteoglycans, and glycosaminoglycans), vimentin, and MSC markers (especially CD73), although isolation and ex vivo culture resulted in a homogeneous cell profile. Three vascular markers were positive in situ, especially vWF, followed by CD34 and CD31, and isolation and culture revealed that the region associated with the highest expression of vascular markers was IV, followed by PV. These results confirm the heterogeneity of the hUC and the need for selecting cells from specific regions of the hUC for particular applications in tissue engineering.https://www.mdpi.com/2075-4426/12/4/648umbilical cordMSCvascular differentiation |
| spellingShingle | Cristina Blanco-Elices Jesús Chato-Astrain Alberto González-González David Sánchez-Porras Víctor Carriel Ricardo Fernández-Valadés María del Carmen Sánchez-Quevedo Miguel Alaminos Ingrid Garzón Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering Journal of Personalized Medicine umbilical cord MSC vascular differentiation |
| title | Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering |
| title_full | Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering |
| title_fullStr | Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering |
| title_full_unstemmed | Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering |
| title_short | Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering |
| title_sort | histological profiling of the human umbilical cord a potential alternative cell source in tissue engineering |
| topic | umbilical cord MSC vascular differentiation |
| url | https://www.mdpi.com/2075-4426/12/4/648 |
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