The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran
Introduction: An increase in the consumption of antibiotics has raised significant concerns over the treatment of Klebsiella pneumonia-infected patients. In this study, the resistance pattern of K. pneumoniae to antibiotics such as imipenem, meropenem, and ertapenem, as well as the frequency of Meta...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2020-01-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | http://jommid.pasteur.ac.ir/article-1-211-en.html |
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| author | Marzieh Saremi Leila Saremi Fatemeh Feizy Somayeh Vafaei Ali Lashkari Zohreh Saltanatpour Razieh Nazari Nazari |
| author_facet | Marzieh Saremi Leila Saremi Fatemeh Feizy Somayeh Vafaei Ali Lashkari Zohreh Saltanatpour Razieh Nazari Nazari |
| author_sort | Marzieh Saremi |
| collection | DOAJ |
| description | Introduction: An increase in the consumption of antibiotics has raised significant concerns over the treatment of Klebsiella pneumonia-infected patients. In this study, the resistance pattern of K. pneumoniae to antibiotics such as imipenem, meropenem, and ertapenem, as well as the frequency of Metallo-beta-lactamase (MBL) genes, namely VIM, IMP, and NDM-1 were investigated. Methods: Following the isolation of 200 K. pneumoniae isolates from 650 clinical samples, the antibiotic resistance pattern of these isolates against different antibiotics was evaluated. The isolates resistant to imipenem, meropenem, and ertapenem were identified, and the presence of VIM, IMP, and NDM-1 genes was examined by using PCR methods. Results: The K. pneumoniae isolates exhibited different resistance patterns in response to various antibiotics. The frequency of VIM, IMP, and NDM-1 genes showed that 48 strains are resistant to imipenem, meropenem, and ertapenem in which 15.6% was positive for IMP, 2.42% for VIM, and 1.92% positive for NDM-1 gene. The isolates showed the highest antibiotic resistance to ampicillin (97.5%) and the lowest to meropenem (5.5%). Conclusion: Considering carbapenem antibiotics such as imipenem, meropenem, and ertapenem which are known to be among the most frequently used antibiotics for the treatment of K. pneumoniae infections and the involvement of MBL genes in this scenario, we aimed to screen and identify MBL genes responsible for the resistance of K. pneumoniae to imipenem, meropenem, and ertapenem. |
| first_indexed | 2024-12-22T19:35:15Z |
| format | Article |
| id | doaj.art-4e09a8d9dcad43e5bd6fc7d2c348c070 |
| institution | Directory Open Access Journal |
| issn | 2345-5349 2345-5330 |
| language | English |
| last_indexed | 2024-12-22T19:35:15Z |
| publishDate | 2020-01-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj.art-4e09a8d9dcad43e5bd6fc7d2c348c0702022-12-21T18:14:59ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302020-01-01813439The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, IranMarzieh Saremi0Leila Saremi1Fatemeh Feizy2Somayeh Vafaei3Ali Lashkari4Zohreh Saltanatpour5Razieh Nazari Nazari6 Department of Microbiology, Qom branch, Islamic Azad University, Qom, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran institute of biochemistry and biophysics university of tehran Medical Genetics Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran 1Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Introduction: An increase in the consumption of antibiotics has raised significant concerns over the treatment of Klebsiella pneumonia-infected patients. In this study, the resistance pattern of K. pneumoniae to antibiotics such as imipenem, meropenem, and ertapenem, as well as the frequency of Metallo-beta-lactamase (MBL) genes, namely VIM, IMP, and NDM-1 were investigated. Methods: Following the isolation of 200 K. pneumoniae isolates from 650 clinical samples, the antibiotic resistance pattern of these isolates against different antibiotics was evaluated. The isolates resistant to imipenem, meropenem, and ertapenem were identified, and the presence of VIM, IMP, and NDM-1 genes was examined by using PCR methods. Results: The K. pneumoniae isolates exhibited different resistance patterns in response to various antibiotics. The frequency of VIM, IMP, and NDM-1 genes showed that 48 strains are resistant to imipenem, meropenem, and ertapenem in which 15.6% was positive for IMP, 2.42% for VIM, and 1.92% positive for NDM-1 gene. The isolates showed the highest antibiotic resistance to ampicillin (97.5%) and the lowest to meropenem (5.5%). Conclusion: Considering carbapenem antibiotics such as imipenem, meropenem, and ertapenem which are known to be among the most frequently used antibiotics for the treatment of K. pneumoniae infections and the involvement of MBL genes in this scenario, we aimed to screen and identify MBL genes responsible for the resistance of K. pneumoniae to imipenem, meropenem, and ertapenem.http://jommid.pasteur.ac.ir/article-1-211-en.htmlklebsiella pneumoniametallo-beta-lactamase genesantibiotic resistancepcr |
| spellingShingle | Marzieh Saremi Leila Saremi Fatemeh Feizy Somayeh Vafaei Ali Lashkari Zohreh Saltanatpour Razieh Nazari Nazari The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran Journal of Medical Microbiology and Infectious Diseases klebsiella pneumonia metallo-beta-lactamase genes antibiotic resistance pcr |
| title | The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran |
| title_full | The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran |
| title_fullStr | The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran |
| title_full_unstemmed | The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran |
| title_short | The Prevalence of VIM, IMP, and NDM-1 Metallo-beta-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Qom Province, Iran |
| title_sort | prevalence of vim imp and ndm 1 metallo beta lactamase genes in clinical isolates of klebsiella pneumoniae in qom province iran |
| topic | klebsiella pneumonia metallo-beta-lactamase genes antibiotic resistance pcr |
| url | http://jommid.pasteur.ac.ir/article-1-211-en.html |
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