Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility
The incorporation of functional groups endows graphene oxide (GO) with different surface charges, which plays important roles in biological interactions with cells. However, the effect of surface charge of GO derivatives on ocular biocompatibility has not been fully elucidated. Previously, we found...
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MDPI AG
2022-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/5/735 |
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| author | Liyuan Rong Yan Fu Qiyou Li Xinji Yang Yueyue Li Liang Yan Liqiang Wang Wei Wu |
| author_facet | Liyuan Rong Yan Fu Qiyou Li Xinji Yang Yueyue Li Liang Yan Liqiang Wang Wei Wu |
| author_sort | Liyuan Rong |
| collection | DOAJ |
| description | The incorporation of functional groups endows graphene oxide (GO) with different surface charges, which plays important roles in biological interactions with cells. However, the effect of surface charge of GO derivatives on ocular biocompatibility has not been fully elucidated. Previously, we found that positively, negatively and neutrally charged PEGylated GO (PEG-GO) nanosheets exerted similar effect on the viability of ocular cells. In this work, we performed in vitro and in vivo studies to comprehensively study the effect of surface charge of PEG-GO on ocular compatibility. The in vitro results showed that the cellular uptake efficacy of negatively charged PEG-GO nanosheets was significantly decreased compared with positively charged and neutrally charged analogs. However, three kinds of PEG-GO nanosheets produced similar amounts of intracellular reactive oxygen species and showed similar influence on mitochondrial membrane potential. By analysis of global gene expression profiles, we found that the correlation coefficients between three kinds of PEG-GO-treated cells were more than 0.98. Furthermore, in vivo results showed that all these PEG-GO nanosheets had no significant toxicity to ocular structure and function. Taken together, our work suggested that surface charge of PEG-GO exerted negligible effect on its ocular compatibility, except for the cellular uptake. Our work is conducive to understanding the relationship between surface charge and biocompatibility of GO derivatives. |
| first_indexed | 2024-03-09T20:28:06Z |
| format | Article |
| id | doaj.art-4e0b3415a3204dccbb69fdba51d430ba |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T20:28:06Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | Nanomaterials |
| spelling | doaj.art-4e0b3415a3204dccbb69fdba51d430ba2023-11-23T23:29:21ZengMDPI AGNanomaterials2079-49912022-02-0112573510.3390/nano12050735Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular CompatibilityLiyuan Rong0Yan Fu1Qiyou Li2Xinji Yang3Yueyue Li4Liang Yan5Liqiang Wang6Wei Wu7Senior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing 100143, ChinaThe General Hospital of Western Theater Command, Chengdu 610000, ChinaDepartment of Ophthalmology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaSenior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing 100143, ChinaSenior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing 100143, ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing 100049, ChinaSenior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing 100143, ChinaSenior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing 100143, ChinaThe incorporation of functional groups endows graphene oxide (GO) with different surface charges, which plays important roles in biological interactions with cells. However, the effect of surface charge of GO derivatives on ocular biocompatibility has not been fully elucidated. Previously, we found that positively, negatively and neutrally charged PEGylated GO (PEG-GO) nanosheets exerted similar effect on the viability of ocular cells. In this work, we performed in vitro and in vivo studies to comprehensively study the effect of surface charge of PEG-GO on ocular compatibility. The in vitro results showed that the cellular uptake efficacy of negatively charged PEG-GO nanosheets was significantly decreased compared with positively charged and neutrally charged analogs. However, three kinds of PEG-GO nanosheets produced similar amounts of intracellular reactive oxygen species and showed similar influence on mitochondrial membrane potential. By analysis of global gene expression profiles, we found that the correlation coefficients between three kinds of PEG-GO-treated cells were more than 0.98. Furthermore, in vivo results showed that all these PEG-GO nanosheets had no significant toxicity to ocular structure and function. Taken together, our work suggested that surface charge of PEG-GO exerted negligible effect on its ocular compatibility, except for the cellular uptake. Our work is conducive to understanding the relationship between surface charge and biocompatibility of GO derivatives.https://www.mdpi.com/2079-4991/12/5/735surface chargegraphene oxidenanotoxicologybiocompatibilitynano-bio interactions |
| spellingShingle | Liyuan Rong Yan Fu Qiyou Li Xinji Yang Yueyue Li Liang Yan Liqiang Wang Wei Wu Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility Nanomaterials surface charge graphene oxide nanotoxicology biocompatibility nano-bio interactions |
| title | Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility |
| title_full | Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility |
| title_fullStr | Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility |
| title_full_unstemmed | Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility |
| title_short | Effects of the Surface Charge of Graphene Oxide Derivatives on Ocular Compatibility |
| title_sort | effects of the surface charge of graphene oxide derivatives on ocular compatibility |
| topic | surface charge graphene oxide nanotoxicology biocompatibility nano-bio interactions |
| url | https://www.mdpi.com/2079-4991/12/5/735 |
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