Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study
With the widespread use of antibacterial drugs and increasing number of immunocompromised patients, pulmonary fungal infections are becoming more common. However, the incidence of pulmonary fungal and bacterial co-infection is rarely reported. In this study, 119 patients definitively diagnosed with...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.749905/full |
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| author | Zhan Zhao Junxiu Song Changqing Yang Lei Yang Jie Chen Xinhui Li Yubao Wang Jing Feng |
| author_facet | Zhan Zhao Junxiu Song Changqing Yang Lei Yang Jie Chen Xinhui Li Yubao Wang Jing Feng |
| author_sort | Zhan Zhao |
| collection | DOAJ |
| description | With the widespread use of antibacterial drugs and increasing number of immunocompromised patients, pulmonary fungal infections are becoming more common. However, the incidence of pulmonary fungal and bacterial co-infection is rarely reported. In this study, 119 patients definitively diagnosed with pulmonary fungal infections between July 2018 and March 2020 were assessed using metagenomic next-generation sequencing (mNGS) as well as traditional pathogen detection to gauge the incidence of fungal and bacterial co-infection and evaluate the associated risk factors. We found that of the 119 patients with fungal infections, 48 (40.3%) had pulmonary fungal and bacterial co-infection. We identified immunocompromised status and the presence of one or more pulmonary cavities as risk factors associated with fungal and bacterial co-infection. The most commonly isolated fungi species were Aspergillus, Pneumocystis, and Rhizopus. The most commonly isolated bacterial species were Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia. Seventy-nine (66.4%) patients had received empirical antibiotic treatment before their pathogenic test results became available, and 41.7% (fungal infection group) and 38.7% (fungal and bacterial co-infection group) of the patients had their antibacterial drug dosage changed accordingly. This mNGS-based study showed that the incidence of fungal and bacterial co-infection is significant. Our research outcomes can, thus, guide the use of antibacterial drugs in the treatment of clinical fungal infections. |
| first_indexed | 2024-12-20T23:32:51Z |
| format | Article |
| id | doaj.art-4e0be129f3bf4b0d9f97b2794758e2cc |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-20T23:32:51Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4e0be129f3bf4b0d9f97b2794758e2cc2022-12-21T19:23:16ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-11-011110.3389/fcimb.2021.749905749905Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based StudyZhan Zhao0Junxiu Song1Changqing Yang2Lei Yang3Jie Chen4Xinhui Li5Yubao Wang6Jing Feng7Respiratory Department, Tianjin Medical University General Hospital, Tianjin, ChinaRespiratory Department, Tianjin Medical University General Hospital, Tianjin, ChinaRespiratory Department, Tianjin Medical University General Hospital, Tianjin, ChinaRespiratory Department, Tianjin Medical University General Hospital, Tianjin, ChinaGuangzhou Sagene Biotechnology Company, Limited, Guangzhou, ChinaGuangzhou Sagene Biotechnology Company, Limited, Guangzhou, ChinaInstitute of Infectious Diseases, The Second Hospital of Tianjin Medical University, Tianjin, ChinaRespiratory Department, Tianjin Medical University General Hospital, Tianjin, ChinaWith the widespread use of antibacterial drugs and increasing number of immunocompromised patients, pulmonary fungal infections are becoming more common. However, the incidence of pulmonary fungal and bacterial co-infection is rarely reported. In this study, 119 patients definitively diagnosed with pulmonary fungal infections between July 2018 and March 2020 were assessed using metagenomic next-generation sequencing (mNGS) as well as traditional pathogen detection to gauge the incidence of fungal and bacterial co-infection and evaluate the associated risk factors. We found that of the 119 patients with fungal infections, 48 (40.3%) had pulmonary fungal and bacterial co-infection. We identified immunocompromised status and the presence of one or more pulmonary cavities as risk factors associated with fungal and bacterial co-infection. The most commonly isolated fungi species were Aspergillus, Pneumocystis, and Rhizopus. The most commonly isolated bacterial species were Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia. Seventy-nine (66.4%) patients had received empirical antibiotic treatment before their pathogenic test results became available, and 41.7% (fungal infection group) and 38.7% (fungal and bacterial co-infection group) of the patients had their antibacterial drug dosage changed accordingly. This mNGS-based study showed that the incidence of fungal and bacterial co-infection is significant. Our research outcomes can, thus, guide the use of antibacterial drugs in the treatment of clinical fungal infections.https://www.frontiersin.org/articles/10.3389/fcimb.2021.749905/fullfungal infectionfungal and bacterial co-infectionrisk factormNGSantibacterial treatment |
| spellingShingle | Zhan Zhao Junxiu Song Changqing Yang Lei Yang Jie Chen Xinhui Li Yubao Wang Jing Feng Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study Frontiers in Cellular and Infection Microbiology fungal infection fungal and bacterial co-infection risk factor mNGS antibacterial treatment |
| title | Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study |
| title_full | Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study |
| title_fullStr | Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study |
| title_full_unstemmed | Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study |
| title_short | Prevalence of Fungal and Bacterial Co-Infection in Pulmonary Fungal Infections: A Metagenomic Next Generation Sequencing-Based Study |
| title_sort | prevalence of fungal and bacterial co infection in pulmonary fungal infections a metagenomic next generation sequencing based study |
| topic | fungal infection fungal and bacterial co-infection risk factor mNGS antibacterial treatment |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.749905/full |
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