Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India
The resistance to MLSB antibiotics, i.e. Macrolide-Lincosamide-Streptogramin B (MLSB), is an increasing problem among Methicillin-resistant Staphylococci. The resistance to macrolides can be by efflux mechanism or via inducible or constitutive resistance. Unfortunately, routine clindamycin susceptib...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2021-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/detection-of-inducible-resistance-to-clindamycin-among-methicillin-resistant-and-sensitive-strains-of-staphylococcus-aureus-from-india/ |
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| author | Rosy Bala Narinder Kaur Nitin Gupta Jyoti Chauhan Ritu Garg Harit Kumar Adesh K Saini |
| author_facet | Rosy Bala Narinder Kaur Nitin Gupta Jyoti Chauhan Ritu Garg Harit Kumar Adesh K Saini |
| author_sort | Rosy Bala |
| collection | DOAJ |
| description | The resistance to MLSB antibiotics, i.e. Macrolide-Lincosamide-Streptogramin B (MLSB), is an increasing problem among Methicillin-resistant Staphylococci. The resistance to macrolides can be by efflux mechanism or via inducible or constitutive resistance. Unfortunately, routine clindamycin susceptibility testing fails to detect the inducible resistance, which commonly results in treatment failure and necessitates incorporating a simple D-test to detect such resistance. A retrospective observational study was performed on S. aureus isolates from patients. The strains were subjected to antibiotic susceptibility testing followed by detection of mecA gene by a polymerase chain reaction and, the ‘D-test’ was performed to know the inducible resistance to clindamycin. A total of 235 isolates were identified as S. aureus. Antibiotic susceptibility test indicated 190 MRSA and 45 are sensitive to MLSB (MS). Inducible clindamycin resistance was found among 48 (20.4%) isolates and constitutive resistance in 104 (44.2%). MRSA strains had higher inducible and constitutive resistance than MSSA strains (22.1%, 51.6% and 13.3%, 13.3%, respectively). Clindamycin is a commonly used antibiotic in patients with MRSA infections to spare higher-end anti-MRSA antibiotics like linezolid and vancomycin. To detect inducible clindamycin to avoid treatment failures; the study showed the importance of incorporating the D-test in routine testing. |
| first_indexed | 2024-12-15T00:01:41Z |
| format | Article |
| id | doaj.art-4e105bce4f6b4dd5a929073ee750a462 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-15T00:01:41Z |
| publishDate | 2021-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-4e105bce4f6b4dd5a929073ee750a4622022-12-21T22:42:53ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2021-12-011541957196210.22207/JPAM.15.4.17Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from IndiaRosy Balahttps://orcid.org/0000-0003-1564-9710Narinder Kaurhttps://orcid.org/0000-0003-4721-8800Nitin Guptahttps://orcid.org/0000-0001-5569-3770Jyoti ChauhanRitu Garghttps://orcid.org/0000-0001-9661-4151Harit Kumarhttps://orcid.org/0000-0002-3684-5043Adesh K Sainihttps://orcid.org/0000-0002-5445-5786The resistance to MLSB antibiotics, i.e. Macrolide-Lincosamide-Streptogramin B (MLSB), is an increasing problem among Methicillin-resistant Staphylococci. The resistance to macrolides can be by efflux mechanism or via inducible or constitutive resistance. Unfortunately, routine clindamycin susceptibility testing fails to detect the inducible resistance, which commonly results in treatment failure and necessitates incorporating a simple D-test to detect such resistance. A retrospective observational study was performed on S. aureus isolates from patients. The strains were subjected to antibiotic susceptibility testing followed by detection of mecA gene by a polymerase chain reaction and, the ‘D-test’ was performed to know the inducible resistance to clindamycin. A total of 235 isolates were identified as S. aureus. Antibiotic susceptibility test indicated 190 MRSA and 45 are sensitive to MLSB (MS). Inducible clindamycin resistance was found among 48 (20.4%) isolates and constitutive resistance in 104 (44.2%). MRSA strains had higher inducible and constitutive resistance than MSSA strains (22.1%, 51.6% and 13.3%, 13.3%, respectively). Clindamycin is a commonly used antibiotic in patients with MRSA infections to spare higher-end anti-MRSA antibiotics like linezolid and vancomycin. To detect inducible clindamycin to avoid treatment failures; the study showed the importance of incorporating the D-test in routine testing.https://microbiologyjournal.org/detection-of-inducible-resistance-to-clindamycin-among-methicillin-resistant-and-sensitive-strains-of-staphylococcus-aureus-from-india/antibioticclindamycinmrsamacrolidemethicillin-resistantstaphylococcusrrna methylase |
| spellingShingle | Rosy Bala Narinder Kaur Nitin Gupta Jyoti Chauhan Ritu Garg Harit Kumar Adesh K Saini Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India Journal of Pure and Applied Microbiology antibiotic clindamycin mrsa macrolide methicillin-resistant staphylococcus rrna methylase |
| title | Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India |
| title_full | Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India |
| title_fullStr | Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India |
| title_full_unstemmed | Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India |
| title_short | Detection of Inducible Resistance to Clindamycin among Methicillin Resistant and Sensitive strains of Staphylococcus aureus from India |
| title_sort | detection of inducible resistance to clindamycin among methicillin resistant and sensitive strains of staphylococcus aureus from india |
| topic | antibiotic clindamycin mrsa macrolide methicillin-resistant staphylococcus rrna methylase |
| url | https://microbiologyjournal.org/detection-of-inducible-resistance-to-clindamycin-among-methicillin-resistant-and-sensitive-strains-of-staphylococcus-aureus-from-india/ |
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