BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
Abstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistoche...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85129-1 |
| _version_ | 1818845372873703424 |
|---|---|
| author | Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic |
| author_facet | Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic |
| author_sort | Louis N. Manganas |
| collection | DOAJ |
| description | Abstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells. |
| first_indexed | 2024-12-19T05:28:37Z |
| format | Article |
| id | doaj.art-4e13a8dbe31b446bb00d9ddc980acb5a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:28:37Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4e13a8dbe31b446bb00d9ddc980acb5a2022-12-21T20:34:18ZengNature PortfolioScientific Reports2045-23222021-03-0111111810.1038/s41598-021-85129-1BASP1 labels neural stem cells in the neurogenic niches of mammalian brainLouis N. Manganas0Irene Durá1Sivan Osenberg2Faith Semerci3Mehmet Tosun4Rachana Mishra5Luke Parkitny6Juan M. Encinas7Mirjana Maletic-Savatic8Department of Neurology, Stony Brook University Medical CenterAchucarro Basque Center for NeuroscienceDepartments of Pediatrics, Neurology and Neuroscience, Baylor College of MedicineDepartments of Pediatrics, Neurology and Neuroscience, Baylor College of MedicineDepartments of Pediatrics, Neurology and Neuroscience, Baylor College of MedicineDepartments of Pediatrics, Neurology and Neuroscience, Baylor College of MedicineDepartments of Pediatrics, Neurology and Neuroscience, Baylor College of MedicineAchucarro Basque Center for NeuroscienceDepartment of Neurology, Stony Brook University Medical CenterAbstract The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells.https://doi.org/10.1038/s41598-021-85129-1 |
| spellingShingle | Louis N. Manganas Irene Durá Sivan Osenberg Faith Semerci Mehmet Tosun Rachana Mishra Luke Parkitny Juan M. Encinas Mirjana Maletic-Savatic BASP1 labels neural stem cells in the neurogenic niches of mammalian brain Scientific Reports |
| title | BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
| title_full | BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
| title_fullStr | BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
| title_full_unstemmed | BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
| title_short | BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
| title_sort | basp1 labels neural stem cells in the neurogenic niches of mammalian brain |
| url | https://doi.org/10.1038/s41598-021-85129-1 |
| work_keys_str_mv | AT louisnmanganas basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT irenedura basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT sivanosenberg basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT faithsemerci basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT mehmettosun basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT rachanamishra basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT lukeparkitny basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT juanmencinas basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain AT mirjanamaleticsavatic basp1labelsneuralstemcellsintheneurogenicnichesofmammalianbrain |