Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774)
Abstract Background The investigation aimed to show the effect of two concentrations of (Protecto 9.4%) commercial formulation of Bacillus thuringiensis kurstaki (Bt) on some biochemical changes of the land snail Monacha cartusiana at three different experimental periods (24, 48 and 72 h). Also, som...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-04-01
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| Series: | Egyptian Journal of Biological Pest Control |
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| Online Access: | https://doi.org/10.1186/s41938-022-00534-6 |
| _version_ | 1818049566845435904 |
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| author | Omhashim Abdelazeim Gaber Abd Elmawgoud Abdalla Asran Fatma Kamel Khider Gamal El-Shahawy Heba Abdel-Tawab Hoda M. K. Elfayoumi |
| author_facet | Omhashim Abdelazeim Gaber Abd Elmawgoud Abdalla Asran Fatma Kamel Khider Gamal El-Shahawy Heba Abdel-Tawab Hoda M. K. Elfayoumi |
| author_sort | Omhashim Abdelazeim Gaber |
| collection | DOAJ |
| description | Abstract Background The investigation aimed to show the effect of two concentrations of (Protecto 9.4%) commercial formulation of Bacillus thuringiensis kurstaki (Bt) on some biochemical changes of the land snail Monacha cartusiana at three different experimental periods (24, 48 and 72 h). Also, some histobiological altrations of the snail at a lethal experimental period of 96 h were studied. Results Pathogenicity effect of two sub-lethal concentrations of biopesticide Protecto; LC20 (6.72 × 106 IU/mg) and LC40 (17.28 × 106 IU/mg) were studied on the land snail M. cartusiana at 3 different exposure periods (24, 48, and 72 h). Some biochemical parameters such as Alkaline phosphatase (ALP), Alanine amino transaminase (ALT), Aspartate amino transaminase (AST), and Total protein (TP) were investigated. These observations cleared that; a significant elevation of ALP, ALT, and AST increased with increasing the sub-lethal concentration of protecto (LC20 and LC40) against the exposed snail. Also, the activity of enzymes significantly increased with increasing the time of exposure (24, 48, and 72 h), respectively. Conversely, the level of TP in the snail was significantly decreased under pathogenic exposure for both (LC20 and LC40) concentrations of Protecto at the same three treated periods (24, 48 and 72 h). The histbiological examinations at LC20 and LC40 for the exposure period 96 h, showed that the digestive gland with vacuolated degenerated, ruptured digestive cells and hemocyte infiltration. Moreover, the foot was observed with necrotic changes, vacuolated connective tissue, as well as, deformation in muscle fiber, and rupture the outer layer. Conclusions Final results showed that protecto B. thuringiensis had a pathogenic effect on land snail enzymatic activities and histbiological structures of land snail. |
| first_indexed | 2024-12-10T10:39:37Z |
| format | Article |
| id | doaj.art-4e13c0b3d487479aa77b03e66019f1da |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-10T10:39:37Z |
| publishDate | 2022-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-4e13c0b3d487479aa77b03e66019f1da2022-12-22T01:52:21ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422022-04-013211810.1186/s41938-022-00534-6Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774)Omhashim Abdelazeim Gaber0Abd Elmawgoud Abdalla Asran1Fatma Kamel Khider2Gamal El-Shahawy3Heba Abdel-Tawab4Hoda M. K. Elfayoumi5Faculty of Science, Department of Zoology, Beni-Suef UniversityPlant Protection Research Institute, Agricultiral Research CenterPlant Protection Research Institute, Agricultiral Research CenterFaculty of Science, Department of Zoology, Beni-Suef UniversityFaculty of Science, Department of Zoology, Beni-Suef UniversityFaculty of Science, Department of Zoology, Beni-Suef UniversityAbstract Background The investigation aimed to show the effect of two concentrations of (Protecto 9.4%) commercial formulation of Bacillus thuringiensis kurstaki (Bt) on some biochemical changes of the land snail Monacha cartusiana at three different experimental periods (24, 48 and 72 h). Also, some histobiological altrations of the snail at a lethal experimental period of 96 h were studied. Results Pathogenicity effect of two sub-lethal concentrations of biopesticide Protecto; LC20 (6.72 × 106 IU/mg) and LC40 (17.28 × 106 IU/mg) were studied on the land snail M. cartusiana at 3 different exposure periods (24, 48, and 72 h). Some biochemical parameters such as Alkaline phosphatase (ALP), Alanine amino transaminase (ALT), Aspartate amino transaminase (AST), and Total protein (TP) were investigated. These observations cleared that; a significant elevation of ALP, ALT, and AST increased with increasing the sub-lethal concentration of protecto (LC20 and LC40) against the exposed snail. Also, the activity of enzymes significantly increased with increasing the time of exposure (24, 48, and 72 h), respectively. Conversely, the level of TP in the snail was significantly decreased under pathogenic exposure for both (LC20 and LC40) concentrations of Protecto at the same three treated periods (24, 48 and 72 h). The histbiological examinations at LC20 and LC40 for the exposure period 96 h, showed that the digestive gland with vacuolated degenerated, ruptured digestive cells and hemocyte infiltration. Moreover, the foot was observed with necrotic changes, vacuolated connective tissue, as well as, deformation in muscle fiber, and rupture the outer layer. Conclusions Final results showed that protecto B. thuringiensis had a pathogenic effect on land snail enzymatic activities and histbiological structures of land snail.https://doi.org/10.1186/s41938-022-00534-6Monacha cartusiana—Protecto—Bacillus thuringiensis (Bt)—Land snailsBiochemical parametersHistobiological alterations |
| spellingShingle | Omhashim Abdelazeim Gaber Abd Elmawgoud Abdalla Asran Fatma Kamel Khider Gamal El-Shahawy Heba Abdel-Tawab Hoda M. K. Elfayoumi Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) Egyptian Journal of Biological Pest Control Monacha cartusiana—Protecto—Bacillus thuringiensis (Bt)—Land snails Biochemical parameters Histobiological alterations |
| title | Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) |
| title_full | Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) |
| title_fullStr | Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) |
| title_full_unstemmed | Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) |
| title_short | Efficacy of biopesticide Protecto (Bacillus thuringiensis) (BT) on certain biochemical activities and histological structures of land snail Monacha cartusiana (Muller, 1774) |
| title_sort | efficacy of biopesticide protecto bacillus thuringiensis bt on certain biochemical activities and histological structures of land snail monacha cartusiana muller 1774 |
| topic | Monacha cartusiana—Protecto—Bacillus thuringiensis (Bt)—Land snails Biochemical parameters Histobiological alterations |
| url | https://doi.org/10.1186/s41938-022-00534-6 |
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