Immune surveillance of the lung by migrating tissue monocytes
Monocytes are phagocytic effector cells in the blood and precursors of resident and inflammatory tissue macrophages. The aim of the current study was to analyse and compare their contribution to innate immune surveillance of the lung in the steady state with macrophage and dendritic cells (DC). ECFP...
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| Language: | English |
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eLife Sciences Publications Ltd
2015-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/07847 |
| _version_ | 1811236805237800960 |
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| author | Mathieu P Rodero Lucie Poupel Pierre-Louis Loyher Pauline Hamon Fabrice Licata Charlotte Pessel David A Hume Christophe Combadière Alexandre Boissonnas |
| author_facet | Mathieu P Rodero Lucie Poupel Pierre-Louis Loyher Pauline Hamon Fabrice Licata Charlotte Pessel David A Hume Christophe Combadière Alexandre Boissonnas |
| author_sort | Mathieu P Rodero |
| collection | DOAJ |
| description | Monocytes are phagocytic effector cells in the blood and precursors of resident and inflammatory tissue macrophages. The aim of the current study was to analyse and compare their contribution to innate immune surveillance of the lung in the steady state with macrophage and dendritic cells (DC). ECFP and EGFP transgenic reporters based upon Csf1r and Cx3cr1 distinguish monocytes from resident mononuclear phagocytes. We used these transgenes to study the migratory properties of monocytes and macrophages by functional imaging on explanted lungs. Migratory monocytes were found to be either patrolling within large vessels of the lung or locating at the interface between lung capillaries and alveoli. This spatial organisation gives to monocytes the property to capture fluorescent particles derived from both vascular and airway routes. We conclude that monocytes participate in steady-state surveillance of the lung, in a way that is complementary to resident macrophages and DC, without differentiating into macrophages. |
| first_indexed | 2024-04-12T12:14:24Z |
| format | Article |
| id | doaj.art-4e188ddf11d44502abdd99e3bfd2288c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:14:24Z |
| publishDate | 2015-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4e188ddf11d44502abdd99e3bfd2288c2022-12-22T03:33:28ZengeLife Sciences Publications LtdeLife2050-084X2015-07-01410.7554/eLife.07847Immune surveillance of the lung by migrating tissue monocytesMathieu P Rodero0Lucie Poupel1Pierre-Louis Loyher2Pauline Hamon3Fabrice Licata4Charlotte Pessel5David A Hume6Christophe Combadière7Alexandre Boissonnas8Centre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceRoyal (Dick) School of Veterinary Studies, The Roslin Institute, Midlothian, United KingdomCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceCentre d'Immunologie et des Maladies Infectieuses, University Pierre et Marie Curie, Sorbonne Universities, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Institut national de la santé et de la recherche médicale, U1135, Paris, France; Centre d'Immunologie et des Maladies Infectieuses, Centre national de la recherche scientifique, ERL 8255, Paris, FranceMonocytes are phagocytic effector cells in the blood and precursors of resident and inflammatory tissue macrophages. The aim of the current study was to analyse and compare their contribution to innate immune surveillance of the lung in the steady state with macrophage and dendritic cells (DC). ECFP and EGFP transgenic reporters based upon Csf1r and Cx3cr1 distinguish monocytes from resident mononuclear phagocytes. We used these transgenes to study the migratory properties of monocytes and macrophages by functional imaging on explanted lungs. Migratory monocytes were found to be either patrolling within large vessels of the lung or locating at the interface between lung capillaries and alveoli. This spatial organisation gives to monocytes the property to capture fluorescent particles derived from both vascular and airway routes. We conclude that monocytes participate in steady-state surveillance of the lung, in a way that is complementary to resident macrophages and DC, without differentiating into macrophages.https://elifesciences.org/articles/07847monocyteinnate immunitylung immunologylive imaging |
| spellingShingle | Mathieu P Rodero Lucie Poupel Pierre-Louis Loyher Pauline Hamon Fabrice Licata Charlotte Pessel David A Hume Christophe Combadière Alexandre Boissonnas Immune surveillance of the lung by migrating tissue monocytes eLife monocyte innate immunity lung immunology live imaging |
| title | Immune surveillance of the lung by migrating tissue monocytes |
| title_full | Immune surveillance of the lung by migrating tissue monocytes |
| title_fullStr | Immune surveillance of the lung by migrating tissue monocytes |
| title_full_unstemmed | Immune surveillance of the lung by migrating tissue monocytes |
| title_short | Immune surveillance of the lung by migrating tissue monocytes |
| title_sort | immune surveillance of the lung by migrating tissue monocytes |
| topic | monocyte innate immunity lung immunology live imaging |
| url | https://elifesciences.org/articles/07847 |
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