Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia
Neuropathic pain is characterized by mechanical allodynia and thermal hyperalgesia to heat, and it affects some 20% of European population. Patients suffering from several neurologic diseases experience neuropathic pain, often finding no relief in therapy. Transgenic mice expressing the gene encodin...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/11/9/985 |
| _version_ | 1797518438297501696 |
|---|---|
| author | Damiana Scuteri Laura Rombolà Silvia Natoli Antonio Pisani Paola Bonsi Kengo Hamamura Giacinto Bagetta Paolo Tonin Maria Tiziana Corasaniti |
| author_facet | Damiana Scuteri Laura Rombolà Silvia Natoli Antonio Pisani Paola Bonsi Kengo Hamamura Giacinto Bagetta Paolo Tonin Maria Tiziana Corasaniti |
| author_sort | Damiana Scuteri |
| collection | DOAJ |
| description | Neuropathic pain is characterized by mechanical allodynia and thermal hyperalgesia to heat, and it affects some 20% of European population. Patients suffering from several neurologic diseases experience neuropathic pain, often finding no relief in therapy. Transgenic mice expressing the gene encoding the human mutant (hMT) or the human wild-type (hWT) torsin A represent a preclinical model of DYT1 dystonia which is the most common form of early-onset inherited dystonia. Baseline thermal sensitivity and hyperalgesia to heat have never been studied in models of dystonia. Therefore, the aim of this research has been to characterize thermal sensitivity in baseline conditions and hyperalgesia to heat after the induction of neuropathic pain through the spinal nerve ligation (SNL) model in mice overexpressing human wild-type and mutated torsin A in comparison to non-transgenic C57BL/6 mice. According to our results, the paw withdrawal latency time to heat in the Hargreaves’ test is significantly lower in the hMT mice (Kruskal–Wallis test = 6.933; <i>p</i> = 0.0312*; hMT vs. hWT <i>p</i> = 0.0317*). On the other hand, no significant differences in SNL-induced thermal hyperalgesia was found among the three strains (Friedman test = 4.933; <i>p</i> = 0.1019). Future studies are needed to better understand the role of torsin A in sensory processing of heat stimuli. |
| first_indexed | 2024-03-10T07:29:52Z |
| format | Article |
| id | doaj.art-4e18d867922140f084bd710c73b8deb9 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T07:29:52Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-4e18d867922140f084bd710c73b8deb92023-11-22T13:56:53ZengMDPI AGLife2075-17292021-09-0111998510.3390/life11090985Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of DystoniaDamiana Scuteri0Laura Rombolà1Silvia Natoli2Antonio Pisani3Paola Bonsi4Kengo Hamamura5Giacinto Bagetta6Paolo Tonin7Maria Tiziana Corasaniti8Preclinical and Translational Pharmacology, Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyPreclinical and Translational Pharmacology, Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyDepartment of Clinical Science and Translational Medicine, University of Rome Tor Vergata, 00133 Rome, ItalyDepartment of Brain and Behavioral Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Brain and Behavioral Sciences, University of Pavia, 27100 Pavia, ItalyLaboratory of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Daiichi University of Pharmacy, Fukuoka 815-8511, JapanPreclinical and Translational Pharmacology, Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, ItalyRegional Center for Serious Brain Injuries, S. Anna Institute, 88900 Crotone, ItalyDepartment of Health Sciences, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, ItalyNeuropathic pain is characterized by mechanical allodynia and thermal hyperalgesia to heat, and it affects some 20% of European population. Patients suffering from several neurologic diseases experience neuropathic pain, often finding no relief in therapy. Transgenic mice expressing the gene encoding the human mutant (hMT) or the human wild-type (hWT) torsin A represent a preclinical model of DYT1 dystonia which is the most common form of early-onset inherited dystonia. Baseline thermal sensitivity and hyperalgesia to heat have never been studied in models of dystonia. Therefore, the aim of this research has been to characterize thermal sensitivity in baseline conditions and hyperalgesia to heat after the induction of neuropathic pain through the spinal nerve ligation (SNL) model in mice overexpressing human wild-type and mutated torsin A in comparison to non-transgenic C57BL/6 mice. According to our results, the paw withdrawal latency time to heat in the Hargreaves’ test is significantly lower in the hMT mice (Kruskal–Wallis test = 6.933; <i>p</i> = 0.0312*; hMT vs. hWT <i>p</i> = 0.0317*). On the other hand, no significant differences in SNL-induced thermal hyperalgesia was found among the three strains (Friedman test = 4.933; <i>p</i> = 0.1019). Future studies are needed to better understand the role of torsin A in sensory processing of heat stimuli.https://www.mdpi.com/2075-1729/11/9/985DYT1torsin Atransgenic miceneuropathic painSNLheat sensitivity |
| spellingShingle | Damiana Scuteri Laura Rombolà Silvia Natoli Antonio Pisani Paola Bonsi Kengo Hamamura Giacinto Bagetta Paolo Tonin Maria Tiziana Corasaniti Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia Life DYT1 torsin A transgenic mice neuropathic pain SNL heat sensitivity |
| title | Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia |
| title_full | Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia |
| title_fullStr | Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia |
| title_full_unstemmed | Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia |
| title_short | Exploitation of Thermal Sensitivity and Hyperalgesia in a Mouse Model of Dystonia |
| title_sort | exploitation of thermal sensitivity and hyperalgesia in a mouse model of dystonia |
| topic | DYT1 torsin A transgenic mice neuropathic pain SNL heat sensitivity |
| url | https://www.mdpi.com/2075-1729/11/9/985 |
| work_keys_str_mv | AT damianascuteri exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT laurarombola exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT silvianatoli exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT antoniopisani exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT paolabonsi exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT kengohamamura exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT giacintobagetta exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT paolotonin exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia AT mariatizianacorasaniti exploitationofthermalsensitivityandhyperalgesiainamousemodelofdystonia |