Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury
Purpose: Erectile dysfunction (ED) is a common postoperative complication of pelvic surgery for which there is currently no effective treatment. This study investigated the therapeutic effects and potential mechanisms of adipose derived mesenchymal stem cells-derived mitochondria (ADSCs-mito) tran...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Sexual Medicine and Andrology
2024-01-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1797382985038692352 |
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| author | Jiancheng Zhai Zehong Chen Peng Chen Wende Yang Hongbo Wei |
| author_facet | Jiancheng Zhai Zehong Chen Peng Chen Wende Yang Hongbo Wei |
| author_sort | Jiancheng Zhai |
| collection | DOAJ |
| description | Purpose: Erectile dysfunction (ED) is a common postoperative complication of pelvic surgery for which there is currently no
effective treatment. This study investigated the therapeutic effects and potential mechanisms of adipose derived mesenchymal
stem cells-derived mitochondria (ADSCs-mito) transplantation in a rat model of bilateral cavernous nerve injury (CNI) ED.
Materials and Methods: We isolated mitochondria from ADSCs and tested their quality. In vivo, twenty male Sprague Dawley
rats were randomly divided into four groups: sham operation group and CNI groups that received intracavernous injection of
either phosphate buffer solution, ADSCs-mito or ADSCs. Two weeks after therapy, the erectile function of the rats was evaluated
and the penile tissues were harvested for histologic analysis and western blotting. In vitro, the apoptosis rate, reactive
oxygen species (ROS), mitochondria derived active oxygen (mtROS) and adenosine triphosphate (ATP) levels were detected
in corpus cavernosum smooth muscle cells (CCSMCs) after the incubation with ADSCs-mito. In addition, intercellular mitochondrial
transfer was visualized by co-culture of ADSCs and CCSMCs.
Results: The ADSCs, ADSCs-mito and CCSMCs were isolated and identified successfully. ADSCs-mito transplantation notably
restored the erectile function and smooth muscle content of CNI ED rats. Moreover, the levels of ROS, mtROS and cleavedcaspase
3 were reduced and the levels of superoxide dismutase and ATP were increased after ADSCs-mito transplantation. In
CNI ED rats, the mitochondrial structure of cells in penile tissues was destroyed. ADSCs could transfer its own mitochondria
to CCSMCs. Pre-treatment with ADSCs-mito could significantly decrease apoptosis rate, ROS levels and mtROS levels as well
as restore the ATP level in CCSMCs.
Conclusions: ADSCs-mito transplantation significantly ameliorated ED induced by CNI, with similar potency to ADSCs treatment.
The ADSCs-mito might exert their effects via anti-oxidative stress, anti-apoptosis and modulating energy metabolism of
CCSMCs. Mitochondrial transplantation should be a promising therapeutic method for treating CNI ED in the future. |
| first_indexed | 2024-03-08T21:14:13Z |
| format | Article |
| id | doaj.art-4e1daa0505d14908886cbfafa63b4248 |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-03-08T21:14:13Z |
| publishDate | 2024-01-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-4e1daa0505d14908886cbfafa63b42482023-12-22T02:46:53ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902024-01-0142118820110.5534/wjmh.220233Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve InjuryJiancheng Zhai0https://orcid.org/0000-0002-9518-234XZehong Chen1https://orcid.org/0000-0002-4754-7965Peng Chen2https://orcid.org/0000-0002-3151-8262Wende Yang3https://orcid.org/0000-0001-9163-2078Hongbo Wei4https://orcid.org/0000-0002-1809-6544Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaPurpose: Erectile dysfunction (ED) is a common postoperative complication of pelvic surgery for which there is currently no effective treatment. This study investigated the therapeutic effects and potential mechanisms of adipose derived mesenchymal stem cells-derived mitochondria (ADSCs-mito) transplantation in a rat model of bilateral cavernous nerve injury (CNI) ED. Materials and Methods: We isolated mitochondria from ADSCs and tested their quality. In vivo, twenty male Sprague Dawley rats were randomly divided into four groups: sham operation group and CNI groups that received intracavernous injection of either phosphate buffer solution, ADSCs-mito or ADSCs. Two weeks after therapy, the erectile function of the rats was evaluated and the penile tissues were harvested for histologic analysis and western blotting. In vitro, the apoptosis rate, reactive oxygen species (ROS), mitochondria derived active oxygen (mtROS) and adenosine triphosphate (ATP) levels were detected in corpus cavernosum smooth muscle cells (CCSMCs) after the incubation with ADSCs-mito. In addition, intercellular mitochondrial transfer was visualized by co-culture of ADSCs and CCSMCs. Results: The ADSCs, ADSCs-mito and CCSMCs were isolated and identified successfully. ADSCs-mito transplantation notably restored the erectile function and smooth muscle content of CNI ED rats. Moreover, the levels of ROS, mtROS and cleavedcaspase 3 were reduced and the levels of superoxide dismutase and ATP were increased after ADSCs-mito transplantation. In CNI ED rats, the mitochondrial structure of cells in penile tissues was destroyed. ADSCs could transfer its own mitochondria to CCSMCs. Pre-treatment with ADSCs-mito could significantly decrease apoptosis rate, ROS levels and mtROS levels as well as restore the ATP level in CCSMCs. Conclusions: ADSCs-mito transplantation significantly ameliorated ED induced by CNI, with similar potency to ADSCs treatment. The ADSCs-mito might exert their effects via anti-oxidative stress, anti-apoptosis and modulating energy metabolism of CCSMCs. Mitochondrial transplantation should be a promising therapeutic method for treating CNI ED in the future.apoptosiserectile dysfunctionmesenchymal stem cellsmitochondriaoxidative stress |
| spellingShingle | Jiancheng Zhai Zehong Chen Peng Chen Wende Yang Hongbo Wei Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury The World Journal of Men's Health apoptosis erectile dysfunction mesenchymal stem cells mitochondria oxidative stress |
| title | Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury |
| title_full | Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury |
| title_fullStr | Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury |
| title_full_unstemmed | Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury |
| title_short | Adipose Derived Mesenchymal Stem Cells-Derived Mitochondria Transplantation Ameliorated Erectile Dysfunction Induced by Cavernous Nerve Injury |
| title_sort | adipose derived mesenchymal stem cells derived mitochondria transplantation ameliorated erectile dysfunction induced by cavernous nerve injury |
| topic | apoptosis erectile dysfunction mesenchymal stem cells mitochondria oxidative stress |
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