The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid
Salicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced diseas...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12197 |
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| author | Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu |
| author_facet | Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu |
| author_sort | Nengxu Sun |
| collection | DOAJ |
| description | Salicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced disease susceptibility 8</i> (<i>EDS8</i>) mutant was compromised in plant defenses to hemibiotrophic pathogen <i>Pseudomonas syringae pv. maculicola</i> ES4326 and necrotrophic pathogen <i>Botrytis cinerea</i>, and was deficient in plant responses to both SA and JA. The <i>EDS8</i> was identified to be <i>THO1</i>, which encodes a subunit of the THO/TREX complex, by using mapping-by-sequencing. To check whether the EDS8 itself or the THO/TREX complex mediates SA and JA signaling, the mutant of another subunit of the THO/TREX complex, THO3, was tested. <i>THO3</i> mutation reduced both SA and JA induced defenses, indicating that the THO/TREX complex is critical for plant responses to these two hormones. We further proved that the THO/TREX interacting protein SERRATE, a factor regulating alternative splicing (AS), was involved in plant responses to SA and JA. Thus, the AS events in the <i>eds</i>8 mutant after SA or JA treatment were determined, and we found that the SA and JA induced different alternative splicing events were majorly modulated by EDS8. In summary, our study proves that the THO/TREX complex active in AS is involved in both SA and JA induced plant defenses. |
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| spelling | doaj.art-4e1f8113d74f4ab6abd31c06b760b5312023-11-22T23:38:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221219710.3390/ijms222212197The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic AcidNengxu Sun0Xiangjiu Kong1Yueyan Liu2Tingting Gong3Xiaoyong Gu4Lijing Liu5The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, ChinaSalicylic acid (SA) and jasmonic acid (JA) are essential plant immune hormones, which could induce plant resistance to multiple pathogens. However, whether common components are employed by both SA and JA to induce defense is largely unknown. In this study, we found that the <i>enhanced disease susceptibility 8</i> (<i>EDS8</i>) mutant was compromised in plant defenses to hemibiotrophic pathogen <i>Pseudomonas syringae pv. maculicola</i> ES4326 and necrotrophic pathogen <i>Botrytis cinerea</i>, and was deficient in plant responses to both SA and JA. The <i>EDS8</i> was identified to be <i>THO1</i>, which encodes a subunit of the THO/TREX complex, by using mapping-by-sequencing. To check whether the EDS8 itself or the THO/TREX complex mediates SA and JA signaling, the mutant of another subunit of the THO/TREX complex, THO3, was tested. <i>THO3</i> mutation reduced both SA and JA induced defenses, indicating that the THO/TREX complex is critical for plant responses to these two hormones. We further proved that the THO/TREX interacting protein SERRATE, a factor regulating alternative splicing (AS), was involved in plant responses to SA and JA. Thus, the AS events in the <i>eds</i>8 mutant after SA or JA treatment were determined, and we found that the SA and JA induced different alternative splicing events were majorly modulated by EDS8. In summary, our study proves that the THO/TREX complex active in AS is involved in both SA and JA induced plant defenses.https://www.mdpi.com/1422-0067/22/22/12197salicylic acidjasmonic acidTHO/TREX complexalternative splicinginduced defense |
| spellingShingle | Nengxu Sun Xiangjiu Kong Yueyan Liu Tingting Gong Xiaoyong Gu Lijing Liu The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid International Journal of Molecular Sciences salicylic acid jasmonic acid THO/TREX complex alternative splicing induced defense |
| title | The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid |
| title_full | The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid |
| title_fullStr | The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid |
| title_full_unstemmed | The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid |
| title_short | The THO/TREX Complex Active in Alternative Splicing Mediates Plant Responses to Salicylic Acid and Jasmonic Acid |
| title_sort | tho trex complex active in alternative splicing mediates plant responses to salicylic acid and jasmonic acid |
| topic | salicylic acid jasmonic acid THO/TREX complex alternative splicing induced defense |
| url | https://www.mdpi.com/1422-0067/22/22/12197 |
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