Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225)
Sequencing surveys of microbial communities in marine subsurface sediments have focused on organic-rich, continental margins; the database for organic-lean deep-sea sediments from mid-ocean regions is underdeveloped. The archaeal community in subsurface sediments of ODP Site 1225 in the eastern equa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Microorganisms |
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| Online Access: | http://www.mdpi.com/2076-2607/4/3/32 |
| _version_ | 1819012858982170624 |
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| author | Antje Lauer Ketil Bernt Sørensen Andreas Teske |
| author_facet | Antje Lauer Ketil Bernt Sørensen Andreas Teske |
| author_sort | Antje Lauer |
| collection | DOAJ |
| description | Sequencing surveys of microbial communities in marine subsurface sediments have focused on organic-rich, continental margins; the database for organic-lean deep-sea sediments from mid-ocean regions is underdeveloped. The archaeal community in subsurface sediments of ODP Site 1225 in the eastern equatorial Pacific (3760 m water depth; 1.1 and 7.8 m sediment depth) was analyzed by PCR, cloning and sequencing, and by denaturant gradient gel electrophoresis (DGGE) of 16S rRNA genes. Three uncultured archaeal lineages with different depth distributions were found: Marine Group I (MG-I) within the Thaumarchaeota, its sister lineage Marine Benthic Group A (MBG-A), the phylum-level archaeal lineage Marine Benthic Group B (also known as Deep-Sea Archaeal Group or Lokiarchaeota), and the Deep-Sea Euryarchaeotal Group 3. The MG-I phylotypes included representatives of sediment clusters that are distinct from the pelagic members of this phylum. On the scale from fully oxidized, extremely organic carbon-depleted sediments (for example, those the South Pacific Gyre) to fully reduced, organic carbon-rich marine subsurface sediments (such as those of the Peru Margin), Ocean Drilling Program (ODP) Site 1225 falls into the non-extreme organic carbon-lean category, and harbors archaeal communities from both ends of the spectrum. |
| first_indexed | 2024-12-21T01:50:44Z |
| format | Article |
| id | doaj.art-4e1fe008d04347b3a4b5645122b777f5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-21T01:50:44Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-4e1fe008d04347b3a4b5645122b777f52022-12-21T19:19:54ZengMDPI AGMicroorganisms2076-26072016-09-01433210.3390/microorganisms4030032microorganisms4030032Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225)Antje Lauer0Ketil Bernt Sørensen1Andreas Teske2Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USADepartment of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USADepartment of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USASequencing surveys of microbial communities in marine subsurface sediments have focused on organic-rich, continental margins; the database for organic-lean deep-sea sediments from mid-ocean regions is underdeveloped. The archaeal community in subsurface sediments of ODP Site 1225 in the eastern equatorial Pacific (3760 m water depth; 1.1 and 7.8 m sediment depth) was analyzed by PCR, cloning and sequencing, and by denaturant gradient gel electrophoresis (DGGE) of 16S rRNA genes. Three uncultured archaeal lineages with different depth distributions were found: Marine Group I (MG-I) within the Thaumarchaeota, its sister lineage Marine Benthic Group A (MBG-A), the phylum-level archaeal lineage Marine Benthic Group B (also known as Deep-Sea Archaeal Group or Lokiarchaeota), and the Deep-Sea Euryarchaeotal Group 3. The MG-I phylotypes included representatives of sediment clusters that are distinct from the pelagic members of this phylum. On the scale from fully oxidized, extremely organic carbon-depleted sediments (for example, those the South Pacific Gyre) to fully reduced, organic carbon-rich marine subsurface sediments (such as those of the Peru Margin), Ocean Drilling Program (ODP) Site 1225 falls into the non-extreme organic carbon-lean category, and harbors archaeal communities from both ends of the spectrum.http://www.mdpi.com/2076-2607/4/3/32Marine archaeaMarine Group Isubsurfacemarine sedimentOcean Drilling Program |
| spellingShingle | Antje Lauer Ketil Bernt Sørensen Andreas Teske Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) Microorganisms Marine archaea Marine Group I subsurface marine sediment Ocean Drilling Program |
| title | Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) |
| title_full | Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) |
| title_fullStr | Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) |
| title_full_unstemmed | Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) |
| title_short | Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225) |
| title_sort | phylogenetic characterization of marine benthic archaea in organic poor sediments of the eastern equatorial pacific ocean odp site 1225 |
| topic | Marine archaea Marine Group I subsurface marine sediment Ocean Drilling Program |
| url | http://www.mdpi.com/2076-2607/4/3/32 |
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